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Research article
Qinan Wu
Corresponding Author
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Background: Aquatic Euryale ferox Salisb. is an economically important crop in China and India. Unfortunately, low yield limitations seriously hinder market growth. Unveiling the control of seed size is of remarkable importance in improvement of crops. Here, we generated a new hybrid line (HL) with larger seeds by crossing South Gordon Euryale and North Gordon Euryale (WT) which hasn’t been reported before. However, the functional genes and molecular mechanisms controlling the seed size in Euryale ferox Salisb. remain unclear. In this study, we focused on the differentially expressed genes in the auxin signal transduction pathway during fruit development between HL and WT to explore candidate regulatory genes participated in regulating seed size.
Results: Both concentration and localization of indole-3-acetic acid (IAA) at two growth stages of fruits of WT and HL were detected by LC-MS and immunofluorescence. Although IAA content between the two lines did not differ, IAA distribution was significantly different. To elucidate the mechanism and to seek the key genes underlying this difference, RNA-seq was performed on young fruits at the two selected growth stages, and differentially expressed genes related to the auxin transduction pathway were selected for further analysis.
Conclusion: Hybrid Euryale ferox Salisb. expressed significant heterosis, resulting in non-prickly, thin-coated, large seeds, which accounted for the significantly larger yield of HL than that of WT. Our study indicated that Small Auxin Up RNAs (SAURs) -mediated localization of IAA regulates seed size in Euryale ferox Salisb. We found that some SAURs may act as a positive mediator of the auxin transduction pathway, thereby contributing to the observed heterosis.
Keywords
Euryale ferox Salisb., hybrid, RNA-seq, IAA, SAUR
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CURRENT STATUS: Published
View the published article at BMC Plant Biology.
Version 4
Posted 05 Jun, 2020
No community comments so far
Editorial decision: Minor revision
On 11 Jun, 2020
Reviewer #1 agreed
On 03 Jun, 2020
Review #1 received
Received 03 Jun, 2020
Reviewers invited
Invitations sent on 01 Jun, 2020
Editor assigned
On 31 May, 2020
Submission checks complete
On 30 May, 2020
Editor invited
On 30 May, 2020
No comments provided
Editorial decision: Major revision
On 19 May, 2020
Reviewer #1 agreed
On 16 May, 2020
Review #1 received
Received 16 May, 2020
Reviewers invited
Invitations sent on 14 May, 2020
Editor assigned
On 13 May, 2020
Submission checks complete
On 12 May, 2020
Editor invited
On 12 May, 2020
No comments provided
Editorial decision: Major revision
On 23 Apr, 2020
Review #1 received
Received 20 Apr, 2020
Reviewer #2 agreed
On 03 Apr, 2020
Review #2 received
Received 03 Apr, 2020
Reviewers invited
Invitations sent on 01 Apr, 2020
Reviewer #1 agreed
On 01 Apr, 2020
Editor assigned
On 31 Mar, 2020
Submission checks complete
On 30 Mar, 2020
Editor invited
On 30 Mar, 2020
No comments provided
Editorial decision: Major revision
On 25 Mar, 2020
Review #3 received
Received 24 Mar, 2020
Reviewer #3 agreed
On 22 Mar, 2020
Reviewer #2 agreed
On 19 Mar, 2020
Review #2 received
Received 19 Mar, 2020
Review #1 received
Received 26 Feb, 2020
Reviewer #1 agreed
On 11 Feb, 2020
Editor assigned
On 21 Jan, 2020
Reviewers invited
Invitations sent on 21 Jan, 2020
Submission checks complete
On 17 Jan, 2020
Editor invited
On 16 Jan, 2020
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See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Qinan Wu
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
Version 4
Posted 05 Jun, 2020
No community comments so far
Editorial decision: Minor revision
On 11 Jun, 2020
Reviewer #1 agreed
On 03 Jun, 2020
Review #1 received
Received 03 Jun, 2020
Reviewers invited
Invitations sent on 01 Jun, 2020
Editor assigned
On 31 May, 2020
Submission checks complete
On 30 May, 2020
Editor invited
On 30 May, 2020
No comments provided
Editorial decision: Major revision
On 19 May, 2020
Reviewer #1 agreed
On 16 May, 2020
Review #1 received
Received 16 May, 2020
Reviewers invited
Invitations sent on 14 May, 2020
Editor assigned
On 13 May, 2020
Submission checks complete
On 12 May, 2020
Editor invited
On 12 May, 2020
No comments provided
Editorial decision: Major revision
On 23 Apr, 2020
Review #1 received
Received 20 Apr, 2020
Reviewer #2 agreed
On 03 Apr, 2020
Review #2 received
Received 03 Apr, 2020
Reviewers invited
Invitations sent on 01 Apr, 2020
Reviewer #1 agreed
On 01 Apr, 2020
Editor assigned
On 31 Mar, 2020
Submission checks complete
On 30 Mar, 2020
Editor invited
On 30 Mar, 2020
No comments provided
Editorial decision: Major revision
On 25 Mar, 2020
Review #3 received
Received 24 Mar, 2020
Reviewer #3 agreed
On 22 Mar, 2020
Reviewer #2 agreed
On 19 Mar, 2020
Review #2 received
Received 19 Mar, 2020
Review #1 received
Received 26 Feb, 2020
Reviewer #1 agreed
On 11 Feb, 2020
Editor assigned
On 21 Jan, 2020
Reviewers invited
Invitations sent on 21 Jan, 2020
Submission checks complete
On 17 Jan, 2020
Editor invited
On 16 Jan, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background: Aquatic Euryale ferox Salisb. is an economically important crop in China and India. Unfortunately, low yield limitations seriously hinder market growth. Unveiling the control of seed size is of remarkable importance in improvement of crops. Here, we generated a new hybrid line (HL) with larger seeds by crossing South Gordon Euryale and North Gordon Euryale (WT) which hasn’t been reported before. However, the functional genes and molecular mechanisms controlling the seed size in Euryale ferox Salisb. remain unclear. In this study, we focused on the differentially expressed genes in the auxin signal transduction pathway during fruit development between HL and WT to explore candidate regulatory genes participated in regulating seed size.
Results: Both concentration and localization of indole-3-acetic acid (IAA) at two growth stages of fruits of WT and HL were detected by LC-MS and immunofluorescence. Although IAA content between the two lines did not differ, IAA distribution was significantly different. To elucidate the mechanism and to seek the key genes underlying this difference, RNA-seq was performed on young fruits at the two selected growth stages, and differentially expressed genes related to the auxin transduction pathway were selected for further analysis.
Conclusion: Hybrid Euryale ferox Salisb. expressed significant heterosis, resulting in non-prickly, thin-coated, large seeds, which accounted for the significantly larger yield of HL than that of WT. Our study indicated that Small Auxin Up RNAs (SAURs) -mediated localization of IAA regulates seed size in Euryale ferox Salisb. We found that some SAURs may act as a positive mediator of the auxin transduction pathway, thereby contributing to the observed heterosis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
This is a list of supplementary files associated with this preprint. Click to download.
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