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Research Article
MicroRNAs Expression Profiles in Platelet-Derived Exosomes From Coronary In-Stent Restenosis and the Potential Markers
Chengchun Tang
Department of Cardiology, Zhongda Hospital affiliated to Southeast University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: We conducted this study was to identify any difference in platelet-derived exosome micro-RNA (microRNA) profile between patients with in-stent restenosis and patients without restenosis using gene sequencing.
Method: Platelet-derived exosomes(PDEs) were isolated through ultracentrifugation and validated by Western blotting, transmission electron microscope and NanoSight analysis. The platelet-derived exosome microRNA expression profile was identified by Illumina Hiseq2000/2500 sequencing, and validated by qRT-PCR. Finally, the potential functions of microRNAs were predicted using miRNA-gene-GO network and miRNA-gene-KEGG network.
Result: We identified several differentially expressed platelet-derived exosome microRNAs. In particular, we found that the microRNA PC-3p-75179_24 was up-regulated, while hsa-miR-133a-3p_R+1 and hsa-miR-589-5p_R-1 were down-regulated in patients with in-stent restenosis which were further validated by qRT-PCR results. Our results also showed their potential miRNA-gene-GO and miRNA-gene-KEGG interaction.
Conclusion: In summary, our study reveals for the first time microRNA expression is altered, in platelet-derived exosomes in patients with in-stent restenosis, with specific up-regulation of microRNA PC-3p-75179_24 and down-regulation of hsa-miR-133a-3p_R+1 and hsa-miR-589-5p_R-1. Our findings supports the need to explore the potential of these microRNAs as biomarkers and the pathological effects on in-stent restenosis.
Keywords
In-stent restenosis (ISR), Platelet-derived Exosomes(PDEs), MicroRNA, qRT-PCR
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On 10 Jan, 2021
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On 10 Jan, 2021
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This preprint is under consideration at BMC Cardiovascular Disorders. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
MicroRNAs Expression Profiles in Platelet-Derived Exosomes From Coronary In-Stent Restenosis and the Potential Markers
Chengchun Tang
Department of Cardiology, Zhongda Hospital affiliated to Southeast University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 Jan, 2021
No community comments so far
Review #1 received
Received 11 Jan, 2021
Reviewer #2 agreed
On 10 Jan, 2021
Reviewer #1 agreed
On 10 Jan, 2021
Reviewer #3 agreed
On 10 Jan, 2021
Reviewer #4 agreed
On 10 Jan, 2021
Reviewers invited
Invitations sent on 10 Jan, 2021
Editor assigned
On 08 Jan, 2021
Editor invited
On 08 Jan, 2021
Submission checks complete
On 08 Jan, 2021
First submitted
On 16 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: We conducted this study was to identify any difference in platelet-derived exosome micro-RNA (microRNA) profile between patients with in-stent restenosis and patients without restenosis using gene sequencing.
Method: Platelet-derived exosomes(PDEs) were isolated through ultracentrifugation and validated by Western blotting, transmission electron microscope and NanoSight analysis. The platelet-derived exosome microRNA expression profile was identified by Illumina Hiseq2000/2500 sequencing, and validated by qRT-PCR. Finally, the potential functions of microRNAs were predicted using miRNA-gene-GO network and miRNA-gene-KEGG network.
Result: We identified several differentially expressed platelet-derived exosome microRNAs. In particular, we found that the microRNA PC-3p-75179_24 was up-regulated, while hsa-miR-133a-3p_R+1 and hsa-miR-589-5p_R-1 were down-regulated in patients with in-stent restenosis which were further validated by qRT-PCR results. Our results also showed their potential miRNA-gene-GO and miRNA-gene-KEGG interaction.
Conclusion: In summary, our study reveals for the first time microRNA expression is altered, in platelet-derived exosomes in patients with in-stent restenosis, with specific up-regulation of microRNA PC-3p-75179_24 and down-regulation of hsa-miR-133a-3p_R+1 and hsa-miR-589-5p_R-1. Our findings supports the need to explore the potential of these microRNAs as biomarkers and the pathological effects on in-stent restenosis.
Figure 1
Figure 2
Figure 3
Figure 4