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Alveolar Epithelial Cells Involved in Pulmonary Vascular Remodeling and Constriction of Hypoxic Pulmonary Hypertension
Zhichao Li
Fourth Military Medical University
Corresponding Author
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Background: Hypoxic pulmonary hypertension (HPH) is a common type of pulmonary hypertension. Alveolar epithelial cells (AECs) are the first to perceive hypoxia of alveolar, however, the role of AECs in HPH remain unclear. HPH is characterized by pulmonary vascular remodeling and constriction. The present study was to whether AECs was involved in pulmonary vascular remodeling and constriction.
Methods: Rat HPH models were built and pulmonary artery smooth cells (PASMCs) and AECs were treatment with hypoxia. Hemodynamic and morphological indicators were measured in samples from rat HPH models. Superoxide dismutase 2 (SOD2), catalase (CAT) and reactive oxygen species (ROS) were detected in AECs or AECs culture medium. To find out the effect of AECs on pulmonary vascular remodeling and constriction, AECs and PASMCs were co-cultured under hypoxia, PASMCs and isolated pulmonary artery (PA) were treatment with AECs hypoxic culture medium. To explore the mechanism of AECs on pulmonary vascular remodeling and constriction, ROS inhibitor N-acetylcysteine (NAC) was used.
Results: In vivo, hypoxia resulted in elevation in pulmonary vascular remodeling and pressure, but had no effect on non-pulmonary vascular. In vitro, hypoxia caused an imbalance of superoxide dismutase 2 (SOD2) and catalase (CAT) and an increase of reactive oxygen species (ROS) in AECs, as well as an increase of hydrogen peroxide (H2O2) in AECs culture medium. Also, AECs led to pulmonary artery smooth cells (PASMCs) proliferation under hypoxia by co-culture or substituting culture medium. AECs hypoxic culture medium enhanced the constriction of isolated pulmonary artery (PA). Further, these responses were abrogated by ROS inhibitor N-acetylcysteine (NAC).
Conclusion: The findings of present study demonstrated that AECs involved in pulmonary vascular remodeling and constriction under hypoxia by secreting H2O2 to the pulmonary microenvironment.
Keywords
alveolar epithelial cells, hypoxic pulmonary hypertension, pulmonary vascular remodeling and constriction, reactive oxygen species
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CURRENT STATUS: Under Revision
Version 1
Posted 18 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 09 Jan, 2021
Review #3 received
Received 06 Jan, 2021
Review #1 received
Received 27 Dec, 2020
Review #2 received
Received 26 Dec, 2020
Reviewer #3 agreed
On 22 Dec, 2020
Reviewer #2 agreed
On 20 Dec, 2020
Reviewers invited
Invitations sent on 20 Dec, 2020
Reviewer #1 agreed
On 20 Dec, 2020
Editor assigned
On 14 Dec, 2020
Submission checks complete
On 14 Dec, 2020
Editor invited
On 14 Dec, 2020
First submitted
On 10 Dec, 2020
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Subject Areas
Pulmonology
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This preprint is under consideration at Respiratory Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Alveolar Epithelial Cells Involved in Pulmonary Vascular Remodeling and Constriction of Hypoxic Pulmonary Hypertension
Zhichao Li
Fourth Military Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 18 Dec, 2020
No community comments so far
Editorial decision: Major revision
On 09 Jan, 2021
Review #3 received
Received 06 Jan, 2021
Review #1 received
Received 27 Dec, 2020
Review #2 received
Received 26 Dec, 2020
Reviewer #3 agreed
On 22 Dec, 2020
Reviewer #2 agreed
On 20 Dec, 2020
Reviewers invited
Invitations sent on 20 Dec, 2020
Reviewer #1 agreed
On 20 Dec, 2020
Editor assigned
On 14 Dec, 2020
Submission checks complete
On 14 Dec, 2020
Editor invited
On 14 Dec, 2020
First submitted
On 10 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Pulmonology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Hypoxic pulmonary hypertension (HPH) is a common type of pulmonary hypertension. Alveolar epithelial cells (AECs) are the first to perceive hypoxia of alveolar, however, the role of AECs in HPH remain unclear. HPH is characterized by pulmonary vascular remodeling and constriction. The present study was to whether AECs was involved in pulmonary vascular remodeling and constriction.
Methods: Rat HPH models were built and pulmonary artery smooth cells (PASMCs) and AECs were treatment with hypoxia. Hemodynamic and morphological indicators were measured in samples from rat HPH models. Superoxide dismutase 2 (SOD2), catalase (CAT) and reactive oxygen species (ROS) were detected in AECs or AECs culture medium. To find out the effect of AECs on pulmonary vascular remodeling and constriction, AECs and PASMCs were co-cultured under hypoxia, PASMCs and isolated pulmonary artery (PA) were treatment with AECs hypoxic culture medium. To explore the mechanism of AECs on pulmonary vascular remodeling and constriction, ROS inhibitor N-acetylcysteine (NAC) was used.
Results: In vivo, hypoxia resulted in elevation in pulmonary vascular remodeling and pressure, but had no effect on non-pulmonary vascular. In vitro, hypoxia caused an imbalance of superoxide dismutase 2 (SOD2) and catalase (CAT) and an increase of reactive oxygen species (ROS) in AECs, as well as an increase of hydrogen peroxide (H2O2) in AECs culture medium. Also, AECs led to pulmonary artery smooth cells (PASMCs) proliferation under hypoxia by co-culture or substituting culture medium. AECs hypoxic culture medium enhanced the constriction of isolated pulmonary artery (PA). Further, these responses were abrogated by ROS inhibitor N-acetylcysteine (NAC).
Conclusion: The findings of present study demonstrated that AECs involved in pulmonary vascular remodeling and constriction under hypoxia by secreting H2O2 to the pulmonary microenvironment.
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