Background: Protein tyrosine kinase 7 (PTK 7) is a membrane receptor, which
can be found in various kinds of cancers . In view of this, detection of PTK 7 in the
peripheral circulation would be an effective way for the early diagnosis of cancer.
Results: In this work, a multi carbon dots and aptamer based signal amplification
ratiometric fluorescence probe was developed . The fluorescence of the aptamer
modified y CDs and b CDs were respectively chosen as the detection signal and interior
label The fluorescence of y CDs was quenched by Fe 3 O 4 and cDNA (complement to
aptamer) compound without PTK 7 but recovered by the addition of PTK 7. Then, t he
free aptamer was cut by DNase Ⅰ, which amplified the detection signal. The ratiometric
fluorescence sensor for PTK 7 was established with the LOD of 0.016 ng mL-1
Conclusions: Summary , a multi carbon dots and aptamer based signal amplification
ratiometric fluorescence probe was developed for the detection of protein tyrosine
kinase 7. The developed probe was applied to PTK 7 detection in MCF 7 cells and
human serum with satisf ying results, thus indicat ing that this probe ha s huge potential
in clinical practice.