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Research Article
Microsecond Pulse Electrical Stimulation Inhibit the Proliferation of Cervical Cancer Cells through the Ras-Raf-MAPK Pathway
Jianliu Wang
Peking University People's Hospital
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Using microsecond pulsed electrical stimulation (μsPES) applied to treat pelvic floor dysfunctions(PFDs) to stimulate the cervical cancer cells. To explore the effects and mechanisms of different μsPES on the proliferation of cervical cancer cells.
Methods: Scheme #1 (1/4/1 Hz, 270/230/270 μs, 30min), scheme #2 (30 Hz, 500 μs, 20min), and scheme #3 (50 Hz, 250 μs, 20min) were selected from a pelvic floor rehabilitation therapy instrument. Three μsPES-based schemes each at 20, 40, 60, 80, and 100 mA current values were used to stimulate SiHa cells and HeLa cells. Cell counting kit-8 (CCK-8) assay was used to detect cell proliferation. Scheme #2 at 100 mA current was employed to stimulate SiHa cells and HeLa cells and then western blotting was applied to detect the expressions of the Ras-Raf-MAPK pathway.
Results: Current with higer frequency and current intensity tended to have a more proliferation inhibition on cervical cancer cells. μsPES had a stronger proliferation inhibition in SiHa cells than HeLa cells. The expressions of MAPK, MEK1/2 and p-MEK1/2, Raf and p-Raf, Ras protein were significantly decreased in SiHa cells and HeLa cells compared with the control. The p-MAPK was up-regulated in HeLa cells while down-regulated in SiHa cells.
Conclusions: μsPES could inhibit the proliferation of cervical cancer cells by suppressing the Ras-Raf-MAPK pathway. The μsPES applied for functional rehabilitation of pelvic floor nerves and muscles is safe for cervical cancer patients at the cellular level
Keywords
μsPES, SiHa cells, HeLa cells, proliferation, Ras-Raf-MAPK pathway
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CURRENT STATUS: Under Review
Version 1
Posted 22 Dec, 2020
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Review #1 received
Received 18 Jan, 2021
Reviewer #3 agreed
On 06 Jan, 2021
Reviewer #4 agreed
On 06 Jan, 2021
Reviewer #1 agreed
On 06 Jan, 2021
Reviewer #2 agreed
On 06 Jan, 2021
Reviewers invited
Invitations sent on 27 Dec, 2020
Editor assigned
On 20 Dec, 2020
Editor invited
On 20 Dec, 2020
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On 20 Dec, 2020
First submitted
On 16 Dec, 2020
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Subject Areas
Cancer Biology
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This preprint is under consideration at BMC Cancer. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Microsecond Pulse Electrical Stimulation Inhibit the Proliferation of Cervical Cancer Cells through the Ras-Raf-MAPK Pathway
Jianliu Wang
Peking University People's Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Dec, 2020
No community comments so far
Review #1 received
Received 18 Jan, 2021
Reviewer #3 agreed
On 06 Jan, 2021
Reviewer #4 agreed
On 06 Jan, 2021
Reviewer #1 agreed
On 06 Jan, 2021
Reviewer #2 agreed
On 06 Jan, 2021
Reviewers invited
Invitations sent on 27 Dec, 2020
Editor assigned
On 20 Dec, 2020
Editor invited
On 20 Dec, 2020
Submission checks complete
On 20 Dec, 2020
First submitted
On 16 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Using microsecond pulsed electrical stimulation (μsPES) applied to treat pelvic floor dysfunctions(PFDs) to stimulate the cervical cancer cells. To explore the effects and mechanisms of different μsPES on the proliferation of cervical cancer cells.
Methods: Scheme #1 (1/4/1 Hz, 270/230/270 μs, 30min), scheme #2 (30 Hz, 500 μs, 20min), and scheme #3 (50 Hz, 250 μs, 20min) were selected from a pelvic floor rehabilitation therapy instrument. Three μsPES-based schemes each at 20, 40, 60, 80, and 100 mA current values were used to stimulate SiHa cells and HeLa cells. Cell counting kit-8 (CCK-8) assay was used to detect cell proliferation. Scheme #2 at 100 mA current was employed to stimulate SiHa cells and HeLa cells and then western blotting was applied to detect the expressions of the Ras-Raf-MAPK pathway.
Results: Current with higer frequency and current intensity tended to have a more proliferation inhibition on cervical cancer cells. μsPES had a stronger proliferation inhibition in SiHa cells than HeLa cells. The expressions of MAPK, MEK1/2 and p-MEK1/2, Raf and p-Raf, Ras protein were significantly decreased in SiHa cells and HeLa cells compared with the control. The p-MAPK was up-regulated in HeLa cells while down-regulated in SiHa cells.
Conclusions: μsPES could inhibit the proliferation of cervical cancer cells by suppressing the Ras-Raf-MAPK pathway. The μsPES applied for functional rehabilitation of pelvic floor nerves and muscles is safe for cervical cancer patients at the cellular level
Figure 1
Figure 2
Figure 3
Figure 4