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Enhancement of Osteogenic Induction by LL37 Modified With Collagen Binding Domain in Vitro and in Vivo
Sui Mai
Sun Yat-Sen University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2278-637X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Bone defect diseases, particularly induced by inflammation, render a challenge for designing ideal drug-loading scaffold that could facilitate bone repairing and eliminate inflammatory pathogens. LL37 is considered as promising alternative loading drug due to its broad-spectrum antimicrobial effect and various bio-functions including osteogenic induction. In this study, we synthesized modified LL37 by adding collagen binding domain (CBD), which aim to provide a specific binding onto collagen and slow-releasing pattern. The modified peptide was proved to exhibit similar biological activities to nature LL37 on rat BMSCs including promoting migration activity, anti-inflammatory activity and osteogenic induction in vitro. Ectopic bone formation experiment further confirmed the angiogenesis and osteoinduction activities in vivo. Collectively, LL37-CBD may be a potential loaded drug for preventative and curative applications in Inflammation-induced bone diseases, exerting dual strategies including anti-inflammatory and osteogenic effects.
Keywords
LL37, collagen binding domain, osteoinduction
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Dec, 2020
No community comments so far
Review #1 received
Received 12 Jan, 2021
Reviewer #2 agreed
On 11 Jan, 2021
Reviewer #1 agreed
On 05 Jan, 2021
Reviewers invited
Invitations sent on 03 Jan, 2021
Editor invited
On 13 Dec, 2020
Editor assigned
On 13 Dec, 2020
Submission checks complete
On 13 Dec, 2020
First submitted
On 10 Dec, 2020
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This preprint is under consideration at Journal of Biological Engineering. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Enhancement of Osteogenic Induction by LL37 Modified With Collagen Binding Domain in Vitro and in Vivo
Sui Mai
Sun Yat-Sen University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2278-637X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Dec, 2020
No community comments so far
Review #1 received
Received 12 Jan, 2021
Reviewer #2 agreed
On 11 Jan, 2021
Reviewer #1 agreed
On 05 Jan, 2021
Reviewers invited
Invitations sent on 03 Jan, 2021
Editor invited
On 13 Dec, 2020
Editor assigned
On 13 Dec, 2020
Submission checks complete
On 13 Dec, 2020
First submitted
On 10 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Bone defect diseases, particularly induced by inflammation, render a challenge for designing ideal drug-loading scaffold that could facilitate bone repairing and eliminate inflammatory pathogens. LL37 is considered as promising alternative loading drug due to its broad-spectrum antimicrobial effect and various bio-functions including osteogenic induction. In this study, we synthesized modified LL37 by adding collagen binding domain (CBD), which aim to provide a specific binding onto collagen and slow-releasing pattern. The modified peptide was proved to exhibit similar biological activities to nature LL37 on rat BMSCs including promoting migration activity, anti-inflammatory activity and osteogenic induction in vitro. Ectopic bone formation experiment further confirmed the angiogenesis and osteoinduction activities in vivo. Collectively, LL37-CBD may be a potential loaded drug for preventative and curative applications in Inflammation-induced bone diseases, exerting dual strategies including anti-inflammatory and osteogenic effects.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.