Identification of molecular markers associated with the progression and prognosis of lung adenocarcinoma:a bioinformatic study
Objective: For the identification of genes of prognostic significance related to tumor microenvironment (TME) in lung adenocarcinoma.
Methods and Materials: Transcriptome data and clinical data of lung adenocarcinoma originated from the Cancer Genome Atlas (TCGA) database. Immune scores and stromal scores were calculated by “Estimation of Stromal and Immune cells in Malignant Tumors using Expression data” algorithm. Based on these calculated scores, the samples were classified as high and low score groups. The average score of respective gene in different groups was calculated. A heat map was created to screen out genes exhibiting differential expressions. The interaction of up-regulated differentially expressed genes (DEGs) and down-regulated DEGs was harvested from a Venn diagram and then covered in the overlapping genes. The core genes influencing prognosis of lung adenocarcinoma were screened out by function enrichment analysis, protein-protein interaction network analysis and Kaplan-Meier (K-M) method on the overlapping genes.
Results: A total of 515 samples of lung adenocarcinoma were harvested from TCGA database. As revealed from the results, a high immune score was related to a high survival rate, while the matrix did not show significant relationships to survival rate. A total of 775 DEGs and 367 overlapping genes were harvested. The functions of these overlapping genes were tightly correlated with DEGs and immune response and were noticeably improved in cytokine-cytokine receptor interaction and chemokine signaling pathway. Eight genes, namely, CCR5, CCR2, CCL14, GNRH2, PKHD1L1, MS4A1, FCER2 and FDCSP, were correlated with prognosis of lung adenocarcinoma.
Conclusion: The genes and pathways affecting prognosis of lung adenocarcinoma were screened out, which offer ideas for subsequent study on lung adenocarcinoma.
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Posted 21 Dec, 2020
On 17 Jan, 2021
Invitations sent on 22 Dec, 2020
On 16 Dec, 2020
On 16 Dec, 2020
On 16 Dec, 2020
On 14 Dec, 2020
Identification of molecular markers associated with the progression and prognosis of lung adenocarcinoma:a bioinformatic study
Posted 21 Dec, 2020
On 17 Jan, 2021
Invitations sent on 22 Dec, 2020
On 16 Dec, 2020
On 16 Dec, 2020
On 16 Dec, 2020
On 14 Dec, 2020
Objective: For the identification of genes of prognostic significance related to tumor microenvironment (TME) in lung adenocarcinoma.
Methods and Materials: Transcriptome data and clinical data of lung adenocarcinoma originated from the Cancer Genome Atlas (TCGA) database. Immune scores and stromal scores were calculated by “Estimation of Stromal and Immune cells in Malignant Tumors using Expression data” algorithm. Based on these calculated scores, the samples were classified as high and low score groups. The average score of respective gene in different groups was calculated. A heat map was created to screen out genes exhibiting differential expressions. The interaction of up-regulated differentially expressed genes (DEGs) and down-regulated DEGs was harvested from a Venn diagram and then covered in the overlapping genes. The core genes influencing prognosis of lung adenocarcinoma were screened out by function enrichment analysis, protein-protein interaction network analysis and Kaplan-Meier (K-M) method on the overlapping genes.
Results: A total of 515 samples of lung adenocarcinoma were harvested from TCGA database. As revealed from the results, a high immune score was related to a high survival rate, while the matrix did not show significant relationships to survival rate. A total of 775 DEGs and 367 overlapping genes were harvested. The functions of these overlapping genes were tightly correlated with DEGs and immune response and were noticeably improved in cytokine-cytokine receptor interaction and chemokine signaling pathway. Eight genes, namely, CCR5, CCR2, CCL14, GNRH2, PKHD1L1, MS4A1, FCER2 and FDCSP, were correlated with prognosis of lung adenocarcinoma.
Conclusion: The genes and pathways affecting prognosis of lung adenocarcinoma were screened out, which offer ideas for subsequent study on lung adenocarcinoma.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8