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Research Article
Isolation and Characterization of a Halophilic Modicisalibacter Sp. Strain Wilcox from Produced Water
Babu Fathepure
Oklahoma State University
Corresponding Author
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We report the isolation a halophilic bacterium that degrades both aromatic and aliphatic hydrocarbons as the sole sources of carbon at high salinity from produced water. Phylogenetic analysis of 16S rRNA-gene sequences shows the isolate is a close relative of Modicisalibacter tunisiensis isolated from an oil-field water in Tunisia. We designate our isolate as Modicisalibacter sp. strain Wilcox. Genome analysis of strain Wilcox revealed the presence of a repertoire of genes involved in the metabolism of both aliphatic and aromatic hydrocarbons. Laboratory studies corroborated the predicted hydrocarbon degradation potential. The strain degraded benzene, toluene, ethylbenzene, and xylenes at salinities ranging from 0.016 to 4.0 M NaCl, with optimal degradation at 1 M NaCl. Also, the strain degraded phenol, benzoate, biphenyl and phenylacetate as the sole sources of carbon at 2.5 M NaCl. Among aliphatic compounds, the strain degraded n-decane and n-hexadecane as the sole sources of carbon at 2.5 M NaCl. Genome analysis also predicted the presence of many heavy metal resistance genes including genes for metal efflux pumps, transport proteins, and enzymatic detoxification. Overall, due to its ability to degrade many hydrocarbons and withstand high salt and heavy metals, strain Wilcox may prove useful for remediation of produced waters.
Keywords
halophilic, aromatic, homologs
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Isolation and Characterization of a Halophilic Modicisalibacter Sp. Strain Wilcox from Produced Water
Babu Fathepure
Oklahoma State University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 31 Dec, 2020
No community comments so far
Review #7 received
Received 06 Jan, 2021
Review #12 received
Received 06 Jan, 2021
Review #6 received
Received 06 Jan, 2021
Review #13 received
Received 06 Jan, 2021
Review #1 received
Received 06 Jan, 2021
Review #3 received
Received 06 Jan, 2021
Review #4 received
Received 06 Jan, 2021
Reviewer #13 agreed
On 02 Jan, 2021
Reviewer #18 agreed
On 02 Jan, 2021
Reviewer #6 agreed
On 02 Jan, 2021
Reviewer #12 agreed
On 02 Jan, 2021
Reviewer #2 agreed
On 02 Jan, 2021
Reviewer #5 agreed
On 02 Jan, 2021
Reviewer #19 agreed
On 02 Jan, 2021
Reviewer #7 agreed
On 02 Jan, 2021
Reviewer #9 agreed
On 02 Jan, 2021
Reviewer #3 agreed
On 02 Jan, 2021
Reviewer #10 agreed
On 02 Jan, 2021
Reviewer #1 agreed
On 02 Jan, 2021
Reviewers invited
Invitations sent on 02 Jan, 2021
Editor assigned
On 02 Jan, 2021
Editor invited
On 29 Dec, 2020
Submission checks complete
On 29 Dec, 2020
First submitted
On 18 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We report the isolation a halophilic bacterium that degrades both aromatic and aliphatic hydrocarbons as the sole sources of carbon at high salinity from produced water. Phylogenetic analysis of 16S rRNA-gene sequences shows the isolate is a close relative of Modicisalibacter tunisiensis isolated from an oil-field water in Tunisia. We designate our isolate as Modicisalibacter sp. strain Wilcox. Genome analysis of strain Wilcox revealed the presence of a repertoire of genes involved in the metabolism of both aliphatic and aromatic hydrocarbons. Laboratory studies corroborated the predicted hydrocarbon degradation potential. The strain degraded benzene, toluene, ethylbenzene, and xylenes at salinities ranging from 0.016 to 4.0 M NaCl, with optimal degradation at 1 M NaCl. Also, the strain degraded phenol, benzoate, biphenyl and phenylacetate as the sole sources of carbon at 2.5 M NaCl. Among aliphatic compounds, the strain degraded n-decane and n-hexadecane as the sole sources of carbon at 2.5 M NaCl. Genome analysis also predicted the presence of many heavy metal resistance genes including genes for metal efflux pumps, transport proteins, and enzymatic detoxification. Overall, due to its ability to degrade many hydrocarbons and withstand high salt and heavy metals, strain Wilcox may prove useful for remediation of produced waters.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.