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Research Article
Comprehensive Profiling of Urinary Peptides in Cow Reveals Physiology Specific Signatures and Several Bioactive Properties
Sudarshan Kumar
National Dairy Research Institute
Corresponding Author
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Peptidomics allows the identification of thousands of peptides that are derived from proteins. Urinary peptidomics has revolutionized the field of diagnostics as the sample represents complete systemic changes of the body and is collected non-invasively. We profiled the peptides in urine collected from different physiological states of Sahiwal cows namely heifer, pregnancy, and lactation. Endogenous peptides were extracted from 30 individual cows belonging to three groups, each group comprising of ten animals (biological replicates n = 10). Nano Liquid chromatography Mass spectrometry (nLC-MS/MS) experiments revealed 5239, 4774, and 5466 peptides in the heifer, pregnant, and lactating animals, respectively. The diversity in endogenous peptides in urine sets baseline information substantiating various bioactivities (anti-inflammatory, antimicrobial, antihypertensive, and anticancerous) associated with cow urine. Several proteases have been traced back in the body which was found responsible for the physiology specific peptide signature of urine. The in silico study also highlights the enrichment of progenitor proteins on the specific chromosome and their relative expression in context to specific physiology. The urinary peptides, precursor proteins, and proteases identified in the study thus set a solid foundation for future research in biomarker discovery and a better understanding of the pathophysiology of the body.
Keywords
biological, urine, bioactive
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Comprehensive Profiling of Urinary Peptides in Cow Reveals Physiology Specific Signatures and Several Bioactive Properties
Sudarshan Kumar
National Dairy Research Institute
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 04 Jan, 2021
No community comments so far
Reviewer #1 agreed
On 21 Jan, 2021
Reviewers invited
Invitations sent on 20 Jan, 2021
Editor assigned
On 12 Jan, 2021
Editor invited
On 30 Dec, 2020
Submission checks complete
On 30 Dec, 2020
First submitted
On 18 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Peptidomics allows the identification of thousands of peptides that are derived from proteins. Urinary peptidomics has revolutionized the field of diagnostics as the sample represents complete systemic changes of the body and is collected non-invasively. We profiled the peptides in urine collected from different physiological states of Sahiwal cows namely heifer, pregnancy, and lactation. Endogenous peptides were extracted from 30 individual cows belonging to three groups, each group comprising of ten animals (biological replicates n = 10). Nano Liquid chromatography Mass spectrometry (nLC-MS/MS) experiments revealed 5239, 4774, and 5466 peptides in the heifer, pregnant, and lactating animals, respectively. The diversity in endogenous peptides in urine sets baseline information substantiating various bioactivities (anti-inflammatory, antimicrobial, antihypertensive, and anticancerous) associated with cow urine. Several proteases have been traced back in the body which was found responsible for the physiology specific peptide signature of urine. The in silico study also highlights the enrichment of progenitor proteins on the specific chromosome and their relative expression in context to specific physiology. The urinary peptides, precursor proteins, and proteases identified in the study thus set a solid foundation for future research in biomarker discovery and a better understanding of the pathophysiology of the body.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.