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Research Article
10-hydroxy-2E-decenoic Acid (10HDA) is not a Caste-differentiation Factor in Melipona Scutellaris Stingless Bees
Ana Bonetti
Institute of Biotechnology, Federal University of Uberlândia
Corresponding Author
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In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved caste differentiation regulation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we analysed the expression of the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A in Melipona scutellaris. We also investigated the metabolic profile of larvae and larval food to search for putative queen-fate inducing compounds. Finally, we assessed the effect of the histone deacetylase inhibitor 10-hydroxy-2E-decenoic acid (10HDA) - the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation - on Melipona caste differentiation. The hdac1, hdac4 and kat2a transcripts were expressed at all stages, with fluctuations in developmental stages and castes, which may be related to endocrine regulation. We did not identify the putative caste-differentiation factors, geraniol and 10HDA. Also, 10HDA was unable to promote differentiation in queens. Our results suggest that epigenetic and hormonal regulations act synergistically for drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.
Keywords
Melipona, epigenetic, hormonal, Scutellaris Stingless
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
10-hydroxy-2E-decenoic Acid (10HDA) is not a Caste-differentiation Factor in Melipona Scutellaris Stingless Bees
Ana Bonetti
Institute of Biotechnology, Federal University of Uberlândia
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 29 Dec, 2020
No community comments so far
Review #4 received
Received 17 Jan, 2021
Review #1 received
Received 17 Jan, 2021
Review #3 received
Received 17 Jan, 2021
Reviewer #3 agreed
On 09 Jan, 2021
Reviewer #2 agreed
On 09 Jan, 2021
Reviewer #7 agreed
On 09 Jan, 2021
Reviewer #4 agreed
On 09 Jan, 2021
Reviewer #6 agreed
On 09 Jan, 2021
Reviewer #1 agreed
On 09 Jan, 2021
Reviewers invited
Invitations sent on 08 Jan, 2021
Editor assigned
On 08 Jan, 2021
Editor invited
On 24 Dec, 2020
Submission checks complete
On 24 Dec, 2020
First submitted
On 18 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved caste differentiation regulation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we analysed the expression of the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A in Melipona scutellaris. We also investigated the metabolic profile of larvae and larval food to search for putative queen-fate inducing compounds. Finally, we assessed the effect of the histone deacetylase inhibitor 10-hydroxy-2E-decenoic acid (10HDA) - the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation - on Melipona caste differentiation. The hdac1, hdac4 and kat2a transcripts were expressed at all stages, with fluctuations in developmental stages and castes, which may be related to endocrine regulation. We did not identify the putative caste-differentiation factors, geraniol and 10HDA. Also, 10HDA was unable to promote differentiation in queens. Our results suggest that epigenetic and hormonal regulations act synergistically for drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.
Figure 1
Figure 2
Figure 3
Figure 4
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.