This preprint is under consideration at BMC Research Notes. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research note
Yasuhiko Matsumoto
Meiji Pharmaceutical University: Meiji Yakka Daigaku
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0796-2304
License:
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Objective: Cutibacterium acnes is a causative agent of inflammatory skin diseases and systemic infections. Systemic infections caused by C. acnes are difficult to treat, and the development of a systemic infection model for C. acnes would be useful for elucidating the mechanisms of infection and searching for therapeutic agents. In this study, we established a silkworm infection model as a new experimental system to evaluate the interaction between C. acnes and the host, and the efficacy of antibacterial drugs.
Results: Silkworms infected with C. acnes died when reared at 37˚C. The dose of injected bacterial cells required to kill half of the silkworms (LD50) was determined under rearing conditions at 37˚C. Silkworms injected with autoclaved C. acnes cells did not die during the study period. The survival time of silkworms injected with C. acnes was prolonged by the injection of antibacterial drugs such as tetracycline and clindamycin. These findings suggest that the silkworm C. acnes infection model can be used to evaluate host toxicity caused by C. acnes and the in vivo efficacy of antimicrobial drugs.
Keywords
anti-bacterial drug, Cutibacterium acnes, silkworm, infection
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Dec, 2020
No community comments so far
Review #2 received
Received 20 Apr, 2021
Editorial decision: Major revision
On 20 Apr, 2021
Review #1 received
Received 15 Apr, 2021
Reviewer #2 agreed
On 06 Apr, 2021
Reviewers invited
Invitations sent on 30 Mar, 2021
Reviewer #1 agreed
On 30 Mar, 2021
Submission checks complete
On 18 Dec, 2020
Editor invited
On 14 Dec, 2020
Editor assigned
On 18 Nov, 2020
First submitted
On 17 Nov, 2020
Metrics
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PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
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This preprint is under consideration at BMC Research Notes. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Yasuhiko Matsumoto
Meiji Pharmaceutical University: Meiji Yakka Daigaku
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0796-2304
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Dec, 2020
No community comments so far
Review #2 received
Received 20 Apr, 2021
Editorial decision: Major revision
On 20 Apr, 2021
Review #1 received
Received 15 Apr, 2021
Reviewer #2 agreed
On 06 Apr, 2021
Reviewers invited
Invitations sent on 30 Mar, 2021
Reviewer #1 agreed
On 30 Mar, 2021
Submission checks complete
On 18 Dec, 2020
Editor invited
On 14 Dec, 2020
Editor assigned
On 18 Nov, 2020
First submitted
On 17 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Cutibacterium acnes is a causative agent of inflammatory skin diseases and systemic infections. Systemic infections caused by C. acnes are difficult to treat, and the development of a systemic infection model for C. acnes would be useful for elucidating the mechanisms of infection and searching for therapeutic agents. In this study, we established a silkworm infection model as a new experimental system to evaluate the interaction between C. acnes and the host, and the efficacy of antibacterial drugs.
Results: Silkworms infected with C. acnes died when reared at 37˚C. The dose of injected bacterial cells required to kill half of the silkworms (LD50) was determined under rearing conditions at 37˚C. Silkworms injected with autoclaved C. acnes cells did not die during the study period. The survival time of silkworms injected with C. acnes was prolonged by the injection of antibacterial drugs such as tetracycline and clindamycin. These findings suggest that the silkworm C. acnes infection model can be used to evaluate host toxicity caused by C. acnes and the in vivo efficacy of antimicrobial drugs.
Figure 1
Figure 2
Figure 3
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