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Evaluation of Anti-Bacterial Drugs Using Silkworms Infected by Cutibacterium Acnes
Yasuhiko Matsumoto
Meiji Pharmaceutical University: Meiji Yakka Daigaku
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0796-2304
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Objective: Cutibacterium acnes is a causative agent of inflammatory skin diseases and systemic infections. Systemic infections caused by C. acnes are difficult to treat, and the development of a systemic infection model for C. acnes would be useful for elucidating the mechanisms of infection and searching for therapeutic agents. In this study, we established a silkworm infection model as a new experimental system to evaluate the interaction between C. acnes and the host, and the efficacy of antibacterial drugs.
Results: Silkworms infected with C. acnes died when reared at 37˚C. The dose of injected bacterial cells required to kill half of the silkworms (LD50) was determined under rearing conditions at 37˚C. Silkworms injected with autoclaved C. acnes cells did not die during the study period. The survival time of silkworms injected with C. acnes was prolonged by the injection of antibacterial drugs such as tetracycline and clindamycin. These findings suggest that the silkworm C. acnes infection model can be used to evaluate host toxicity caused by C. acnes and the in vivo efficacy of antimicrobial drugs.
Keywords
anti-bacterial drug, Cutibacterium acnes, silkworm, infection
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This preprint is under consideration at BMC Research Notes. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Evaluation of Anti-Bacterial Drugs Using Silkworms Infected by Cutibacterium Acnes
Yasuhiko Matsumoto
Meiji Pharmaceutical University: Meiji Yakka Daigaku
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0796-2304
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 22 Dec, 2020
No community comments so far
Submission checks complete
On 18 Dec, 2020
Editor invited
On 14 Dec, 2020
First submitted
On 17 Nov, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Cutibacterium acnes is a causative agent of inflammatory skin diseases and systemic infections. Systemic infections caused by C. acnes are difficult to treat, and the development of a systemic infection model for C. acnes would be useful for elucidating the mechanisms of infection and searching for therapeutic agents. In this study, we established a silkworm infection model as a new experimental system to evaluate the interaction between C. acnes and the host, and the efficacy of antibacterial drugs.
Results: Silkworms infected with C. acnes died when reared at 37˚C. The dose of injected bacterial cells required to kill half of the silkworms (LD50) was determined under rearing conditions at 37˚C. Silkworms injected with autoclaved C. acnes cells did not die during the study period. The survival time of silkworms injected with C. acnes was prolonged by the injection of antibacterial drugs such as tetracycline and clindamycin. These findings suggest that the silkworm C. acnes infection model can be used to evaluate host toxicity caused by C. acnes and the in vivo efficacy of antimicrobial drugs.
Figure 1
Figure 2
Figure 3