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Research
Ayesha Shaikh
Dr Babasaheb Ambedkar Marathwada University
Corresponding Author
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Background: Ficus religiosa is widely present in India especially in northern region and has been traditionally used for various indications. We investigated the anticancer potential of leaf extract against human breast cancer cell line MDA-MB-231. The anticancer potential was further supported by identifying potential phytoconstituents of the extract by HR-LCMS analysis.
Methods: Amongst the collected fractions, subfraction of chloroform was selected for anticancer screening in parallel for identification of phytochemicals by using HR-LCMS. Extract showed positive results in a battery of two tests employed in the current study by using MDA-MB231 breast cancer cell lines. In trypan blue exclusion dye test, 50% of cell killing was observed at 110 µg/ML. In vitro anticancer activity by using SRB assay showed dose dependent growth inhibition at 62.5, 125, 250 and 500 µg/mL concentrations. The parameters LC50, GI and TGI50 found from the assay were 100, 200, 300 µg/mL respectively. Assay parameter In case of HR-LCMS analysis several active phytochemicals were identified in the extract.
Results: F. religiosa leaf extract showed promising anticancer activity against human breast cancer cell MDA-MB-231.
Conclusion: F. religiosa leaf extract has promising anticancer potential against human breast cancer cells MDA-MB-231 and numerous phytochemicals further warrants evaluating it for multi-therapeutic potential.
Keywords
F. religiosa, SRB, HR-LCMS, MDA-MB-231, Anticancer, Secondary Metabolites
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This is a preprint. It has not completed peer review.
Research
Ayesha Shaikh
Dr Babasaheb Ambedkar Marathwada University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 22 Dec, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Internal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Ficus religiosa is widely present in India especially in northern region and has been traditionally used for various indications. We investigated the anticancer potential of leaf extract against human breast cancer cell line MDA-MB-231. The anticancer potential was further supported by identifying potential phytoconstituents of the extract by HR-LCMS analysis.
Methods: Amongst the collected fractions, subfraction of chloroform was selected for anticancer screening in parallel for identification of phytochemicals by using HR-LCMS. Extract showed positive results in a battery of two tests employed in the current study by using MDA-MB231 breast cancer cell lines. In trypan blue exclusion dye test, 50% of cell killing was observed at 110 µg/ML. In vitro anticancer activity by using SRB assay showed dose dependent growth inhibition at 62.5, 125, 250 and 500 µg/mL concentrations. The parameters LC50, GI and TGI50 found from the assay were 100, 200, 300 µg/mL respectively. Assay parameter In case of HR-LCMS analysis several active phytochemicals were identified in the extract.
Results: F. religiosa leaf extract showed promising anticancer activity against human breast cancer cell MDA-MB-231.
Conclusion: F. religiosa leaf extract has promising anticancer potential against human breast cancer cells MDA-MB-231 and numerous phytochemicals further warrants evaluating it for multi-therapeutic potential.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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