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Characterization of Chromosome Constitution in Three Wheat-Thinopyrum intermedium Amphiploids Revealed Frequent Rearrangement of Alien and Wheat Chromosomes
Xingfeng Li
Shandong Agricultural University
Corresponding Author
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Background: Thinopyrum intermedium (2n=6x=42) is an important wild perennial Triticeae species exhibiting many potentially favorable traits for wheat improvement. Wheat-Th. intermedium partial amphiploids serve as a bridge to transfer desirable genes from Th. intermedium into common wheat.
Results: Three octoploid Trititrigia accessions (TE261-1, TE266-1, and TE346-1) with good resistances to stripe rust, powdery mildew and aphids were selected from hybrid progenies between Th. intermedium and the common wheat variety ‘Yannong 15’ (YN15). Genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH) and multicolor GISH (McGISH) analyses demonstrated that the three octoploid Trititrigia possess 42 wheat chromosomes and 14 Th. intermedium chromosomes. The 14 alien (Th. intermedium) chromosomes belong to a mixed genome consisting of J-, JS- and St-genome chromosomes rather than a single J, JS or St genome. Different types of chromosomal structural variation were also detected in the 1A, 6A, 6B, 2D and 7D chromosomes via FISH, McGISH and molecular marker analysis. The identity of the alien chromosomes and the variationes in the wheat chromosomes in the three Trititrigia octoploids were also different.
Conclusions: The wheat-Th. intermedium partial amphiploids possess 14 alien chromosomes which belong to a mixed genome consisting of J-, JS- and St- chromosomes, and 42 wheat chromosomes with different structural variations. These accessions could be used as genetic resources in wheat breeding for the transfer of pest resistance genes from Th. intermedium to common wheat.
Keywords
Th. intermedium, amphiploid, in situ hybridization, molecular markers
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- OriginalgelimagesofsupplementaryFig.23and4.pptx
Supplemental Fig. 1. Chromosomal configuration and GISH results for PMC MI in TE261-1, TE266-1 and TE346-1. Supplemental Fig. 2. Patterns of PCR amplification with St-chromosome-specific molecular markers Supplemental Fig. 3. Patterns of PCR amplification with E-chromosome-specific molecular markers Supplemental Fig. 4. Results of the amplification of wheat chromosome-specific markers. Supplemental Table 1. Chromosomal configurations of TE261-1, TE266 and TE346-1 at PMC MI. Supplemental Table 2. St-chromosome-specific molecular markers used in the study (Hu et al. 2014). Supplemental Table 3. E-chromosome-specific molecular markers used in the study (Hu et al. 2012).
- Supplementary511.docx
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Characterization of Chromosome Constitution in Three Wheat-Thinopyrum intermedium Amphiploids Revealed Frequent Rearrangement of Alien and Wheat Chromosomes
Xingfeng Li
Shandong Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 23 Dec, 2020
No community comments so far
Review #1 received
Received 05 Jan, 2021
Editorial decision: Major revision
On 05 Jan, 2021
Review #2 received
Received 28 Dec, 2020
Reviewer #2 agreed
On 20 Dec, 2020
Reviewers invited
Invitations sent on 16 Dec, 2020
Reviewer #1 agreed
On 16 Dec, 2020
Editor assigned
On 14 Dec, 2020
Submission checks complete
On 14 Dec, 2020
Editor invited
On 14 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Thinopyrum intermedium (2n=6x=42) is an important wild perennial Triticeae species exhibiting many potentially favorable traits for wheat improvement. Wheat-Th. intermedium partial amphiploids serve as a bridge to transfer desirable genes from Th. intermedium into common wheat.
Results: Three octoploid Trititrigia accessions (TE261-1, TE266-1, and TE346-1) with good resistances to stripe rust, powdery mildew and aphids were selected from hybrid progenies between Th. intermedium and the common wheat variety ‘Yannong 15’ (YN15). Genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH) and multicolor GISH (McGISH) analyses demonstrated that the three octoploid Trititrigia possess 42 wheat chromosomes and 14 Th. intermedium chromosomes. The 14 alien (Th. intermedium) chromosomes belong to a mixed genome consisting of J-, JS- and St-genome chromosomes rather than a single J, JS or St genome. Different types of chromosomal structural variation were also detected in the 1A, 6A, 6B, 2D and 7D chromosomes via FISH, McGISH and molecular marker analysis. The identity of the alien chromosomes and the variationes in the wheat chromosomes in the three Trititrigia octoploids were also different.
Conclusions: The wheat-Th. intermedium partial amphiploids possess 14 alien chromosomes which belong to a mixed genome consisting of J-, JS- and St- chromosomes, and 42 wheat chromosomes with different structural variations. These accessions could be used as genetic resources in wheat breeding for the transfer of pest resistance genes from Th. intermedium to common wheat.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5