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Research Article
Effectiveness of Short-Range UV-LED Irradiation in Postmenopausal Osteoporosis Using Ovariectomized Mice
Yoshihiro Nishida
Nagoya University Hospital
Corresponding Author
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Postmenopausal osteoporosis is crucial condition that reduces the QOL of affected patients just like aged type osteoporosis. The aim of this study was to evaluate the effectiveness of short-range UV-LED irradiation in postmenopausal osteoporosis using ovariectomized mice. Preliminary experiments identified the time of onset of osteoporosis after ovariectomy (8 weeks) in our model. We have set up a total of 4 groups (n=8/group); vitamin D-repletion with UV irradiation (Vit.D+UV+), vitamin D-repletion without UV irradiation (Vit.D+UV-), vitamin D-deficiency with UV irradiation (Vit.D-UV+), vitamin D-deficiency without UV irradiation (Vit.D-UV-), and. From 8 weeks after ovariectomy, UV was irradiated for 24 weeks. At the time of 16 and 24 weeks’ irradiation, serum Vit.D levels, various markers of bone metabolism, bone mineral density, and bone strength were evaluated, and histological analyses were performed. In addition, muscle strength was analyzed. Serum 25 (OH) D levels at 40 and 48 weeks of age were increased in the Vit.D-UV + group compared to the Vit.D-UV-group. Cortical thickness evaluated with micro-CT and strength of bone were significantly higher in Vit.D-UV+ group than those in Vit.D-UV- group. There was no difference in muscle strength between Vit.D-UV+ group and Vit.D-UV- group. No obvious adverse effects were observed in UV-irradiated mice including skin findings. Short-range UV irradiation may ameliorate postmenopausal osteoporosis associated with a state of vitamin D deficiency.
Keywords
Vitamin D, Light emitting diode, postmenopausal osteoporosis, Bone morphology, Musculoskeletal system
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Barb Sprangers
commented on 14 January, 2021
Will the LED-UV unit be available for trials soon for those like me that have been diagnosed with osteoporosis? I would love to find out about it when it's available in USA.
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CURRENT STATUS: Under Review
Version 1
Posted 28 Dec, 2020
Community comments: 1
Reviewer #1 agreed
On 25 Jan, 2021
Reviewer #2 agreed
On 25 Jan, 2021
Reviewer #3 agreed
On 25 Jan, 2021
Reviewers invited
Invitations sent on 13 Jan, 2021
Editor assigned
On 12 Jan, 2021
Editor invited
On 21 Dec, 2020
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On 21 Dec, 2020
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Subject Areas
Orthopedic Surgery
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Effectiveness of Short-Range UV-LED Irradiation in Postmenopausal Osteoporosis Using Ovariectomized Mice
Yoshihiro Nishida
Nagoya University Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 28 Dec, 2020
Community comments: 1
Reviewer #1 agreed
On 25 Jan, 2021
Reviewer #2 agreed
On 25 Jan, 2021
Reviewer #3 agreed
On 25 Jan, 2021
Reviewers invited
Invitations sent on 13 Jan, 2021
Editor assigned
On 12 Jan, 2021
Editor invited
On 21 Dec, 2020
Submission checks complete
On 21 Dec, 2020
First submitted
On 19 Dec, 2020
Metrics
Comments: 1
PDF Downloads: ...
HTML Views: ...
Subject Areas
Orthopedic Surgery
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Postmenopausal osteoporosis is crucial condition that reduces the QOL of affected patients just like aged type osteoporosis. The aim of this study was to evaluate the effectiveness of short-range UV-LED irradiation in postmenopausal osteoporosis using ovariectomized mice. Preliminary experiments identified the time of onset of osteoporosis after ovariectomy (8 weeks) in our model. We have set up a total of 4 groups (n=8/group); vitamin D-repletion with UV irradiation (Vit.D+UV+), vitamin D-repletion without UV irradiation (Vit.D+UV-), vitamin D-deficiency with UV irradiation (Vit.D-UV+), vitamin D-deficiency without UV irradiation (Vit.D-UV-), and. From 8 weeks after ovariectomy, UV was irradiated for 24 weeks. At the time of 16 and 24 weeks’ irradiation, serum Vit.D levels, various markers of bone metabolism, bone mineral density, and bone strength were evaluated, and histological analyses were performed. In addition, muscle strength was analyzed. Serum 25 (OH) D levels at 40 and 48 weeks of age were increased in the Vit.D-UV + group compared to the Vit.D-UV-group. Cortical thickness evaluated with micro-CT and strength of bone were significantly higher in Vit.D-UV+ group than those in Vit.D-UV- group. There was no difference in muscle strength between Vit.D-UV+ group and Vit.D-UV- group. No obvious adverse effects were observed in UV-irradiated mice including skin findings. Short-range UV irradiation may ameliorate postmenopausal osteoporosis associated with a state of vitamin D deficiency.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Barb Sprangers
commented on 14 January, 2021
Will the LED-UV unit be available for trials soon for those like me that have been diagnosed with osteoporosis? I would love to find out about it when it's available in USA.