Background: Angelica sinensis (Oliv.) Diels (A. sinensis), the commonly used famous-region Chinese herbs, grew in different geographical locations which were crucial to active components accumulation in medicinal plants. Rhizosphere microbiome played a critical role in plant performance, including nutrient acquisition, growth and development and plant diseases resistance etc., there were still few studies on how microbiome effecting on accumulation of active components of A. sinensis. This study aim to illustrate whether the variations of rhizosphere microbial communities and metabolites profilings of A. sinensis were dependant on soil microenvironments. Soils from the two main A. sinensis producing areas, Gansu and Yunnan Province, were selected for a pot experiment, and then divided into two parts, one part was sterilized and the other did not, planting with Gansu danggui 90-01, allowed to grow for 180 days. After the trial radix A. sinensis were collected for analysis of growth targets and chemical compositions, rhizosphere soils for microbial analyses.
Results: The metabolic profiles and rhizosphere microbial communities of A. sinensis grown under different soil microenvironments showed similar variation. The GN (Gansu non-sterilized), YN (Yunnan non-sterilized), GS (Gansu sterilized), and YS (Yunnan sterilized) group were significantly separated. Specifically, compared with Yunnan soil, antagonistic bacteria such as Sphingomonas, Pseudomonas, Lysobacter, Pseudoxanthomonas, etc. were significantly (p <0.05) enriched in Gansu soil. The characteristic constituents of senkyunolide I and ligustilide dimers enriched in GS group has an extremely positive correlation with Pseudomonas parafulva; organic acids (including chlorogenic acid, dicaffeoylquinic acid and 5-feruloylquinic acid) and their ester coniferyl ferulate enriched in YS Group were significantly positively relevant with Gemmatimonadetes bacterium WY71 and Mucilaginibater sp., respectively.
Conclusions: Given that soil microenvironments may contribute to growth and active components accumulation of A. sinensis. Further study aimes at exploring the functional characteristics of quality-related bacterias, and finding the main response genes and the interactions between genes and the environments, to elucidate the main mechanism of genuine A. sinensis quality formation.