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Chronic Exposure to PM2.5 Aggravates SLE Manifestations in Lupus-Prone Mice
Victor Yuji Yariwake
School of Medicine, University of São Paulo
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0639-1228
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Air pollution causes negative impacts on health. Systemic lupus erythematosus (SLE) is an autoimmune disease with diverse clinical manifestations and multifactorial etiology. Recent studies suggest that air pollution can trigger SLE and induce disease activity. However, this association has not been deeply investigated. Thus, the aim of this study was to evaluate whether exposure to fine particulate matter (PM2.5) exacerbates SLE manifestations, focusing on renal complications, in a lupus-prone animal model. Female NZBWF1 mice were exposed daily to 600µg/m³ of inhaled concentrated ambient particles (CAP) or filtered air (FA). Survival rate, body weight, weight of organs (kidney, spleen, thymus, liver and heart), blood cell count, proteinuria, kidney stereology, renal histopathology, gene expression and oxidative stress were analyzed.
Results: Female NZBW mice exposed to CAP presented decreased survival, increased circulating neutrophils, early onset of proteinuria and increased kidney weight with renal cortex enlargement when compared to NZBW mice exposed to FA.
Conclusions: This work shows that air pollution aggravates some SLE manifestations in lupus-prone mice. These results reinforce the need of reducing air pollutant levels in order to promote a better quality of life for individuals diagnosed with SLE.
Keywords
Air pollution, Systemic lupus erythematosus, Particulate matter, Lupus nephritis, Autoimmunity, Environmental exposure
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CURRENT STATUS: Under Review
Version 1
Posted 31 Dec, 2020
No community comments so far
Reviewer #3 agreed
On 03 Jan, 2021
Reviewer #2 agreed
On 29 Dec, 2020
Reviewer #1 agreed
On 28 Dec, 2020
Reviewers invited
Invitations sent on 23 Dec, 2020
Editor assigned
On 22 Dec, 2020
Submission checks complete
On 22 Dec, 2020
Editor invited
On 22 Dec, 2020
First submitted
On 21 Dec, 2020
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Subject Areas
Toxicology
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This preprint is under consideration at Particle and Fibre Toxicology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Chronic Exposure to PM2.5 Aggravates SLE Manifestations in Lupus-Prone Mice
Victor Yuji Yariwake
School of Medicine, University of São Paulo
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0639-1228
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 31 Dec, 2020
No community comments so far
Reviewer #3 agreed
On 03 Jan, 2021
Reviewer #2 agreed
On 29 Dec, 2020
Reviewer #1 agreed
On 28 Dec, 2020
Reviewers invited
Invitations sent on 23 Dec, 2020
Editor assigned
On 22 Dec, 2020
Submission checks complete
On 22 Dec, 2020
Editor invited
On 22 Dec, 2020
First submitted
On 21 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Toxicology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Air pollution causes negative impacts on health. Systemic lupus erythematosus (SLE) is an autoimmune disease with diverse clinical manifestations and multifactorial etiology. Recent studies suggest that air pollution can trigger SLE and induce disease activity. However, this association has not been deeply investigated. Thus, the aim of this study was to evaluate whether exposure to fine particulate matter (PM2.5) exacerbates SLE manifestations, focusing on renal complications, in a lupus-prone animal model. Female NZBWF1 mice were exposed daily to 600µg/m³ of inhaled concentrated ambient particles (CAP) or filtered air (FA). Survival rate, body weight, weight of organs (kidney, spleen, thymus, liver and heart), blood cell count, proteinuria, kidney stereology, renal histopathology, gene expression and oxidative stress were analyzed.
Results: Female NZBW mice exposed to CAP presented decreased survival, increased circulating neutrophils, early onset of proteinuria and increased kidney weight with renal cortex enlargement when compared to NZBW mice exposed to FA.
Conclusions: This work shows that air pollution aggravates some SLE manifestations in lupus-prone mice. These results reinforce the need of reducing air pollutant levels in order to promote a better quality of life for individuals diagnosed with SLE.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10