This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
In animals, the offspring sex ratio is modulated by kin conflict and cooperation, and determining the ratio is a main concern in evolutionary biology. Male competition for access to local mates is predictive of a female-biased sex ratio in the offspring (local mate competition; LMC). Conversely, female competition for access to local resources is predictive of a male-biased sex ratio in the offspring (local resource competition; LRC). However, several factors other than competition should synergistically operate in real-world populations. In the Australian paper wasp Ropalidia plebeiana, LRC and local resource enhancement (LRE) may operate simultaneously. To determine whether this is the case, we evaluated colony sex ratios and examined whether competition and/or enhancement operates at the population level in this species.
Results
In spring, many foundress queens started their colonies by comb-cutting, in which nest combs from the previous season were divided into several combs to be reused. Genetic relatedness among foundresses did not differ before and after comb-cutting. Relatedness among foundresses was 0.339, whereas relatedness among new foundresses was 0.589, revealing nearly functional monogyny. The global FST value calculated with mtDNA markers was higher than that calculated with microsatellite markers, even after we corrected for differences in effective population sizes between sexes. This finding indicates female philopatry, which was also confirmed by mark–release–recapture before and after the hibernation of new foundresses. The colony sex ratio of reproductives became slightly biased toward males in larger colonies. In addition, both the number of foundresses and number of workers were positively associated with the number of reproductives, which indicates that LRE was also operating.
Conclusions
Our results suggest that although the population structure seems to meet the requirements of LRC, the sex ratio is not modulated solely by LRC. Instead, the availability of female helpers at the founding stage likely mitigates the sex ratio predicted by LRC through LRE. Thus, LRC at the founding stage and LRE at the reproductive stage synergistically modulate the colony sex ratio in R. plebeiana.
Keywords
Sex ratio, enhancement, eusocial wasp, female philopatry
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- FigS1Barplot.eps
Fig. S1. Population assignments from STRUCTURE based on microsatellite data. The figure was drawn at K = 2 for 148 individuals.
- TableS1populations.docx
- TableS2primers.docx
- TableS3GLM.docx
- TableS4.docx
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 05 Jan, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Animal Science
Learn more about our company.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 05 Jan, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Animal Science
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
In animals, the offspring sex ratio is modulated by kin conflict and cooperation, and determining the ratio is a main concern in evolutionary biology. Male competition for access to local mates is predictive of a female-biased sex ratio in the offspring (local mate competition; LMC). Conversely, female competition for access to local resources is predictive of a male-biased sex ratio in the offspring (local resource competition; LRC). However, several factors other than competition should synergistically operate in real-world populations. In the Australian paper wasp Ropalidia plebeiana, LRC and local resource enhancement (LRE) may operate simultaneously. To determine whether this is the case, we evaluated colony sex ratios and examined whether competition and/or enhancement operates at the population level in this species.
Results
In spring, many foundress queens started their colonies by comb-cutting, in which nest combs from the previous season were divided into several combs to be reused. Genetic relatedness among foundresses did not differ before and after comb-cutting. Relatedness among foundresses was 0.339, whereas relatedness among new foundresses was 0.589, revealing nearly functional monogyny. The global FST value calculated with mtDNA markers was higher than that calculated with microsatellite markers, even after we corrected for differences in effective population sizes between sexes. This finding indicates female philopatry, which was also confirmed by mark–release–recapture before and after the hibernation of new foundresses. The colony sex ratio of reproductives became slightly biased toward males in larger colonies. In addition, both the number of foundresses and number of workers were positively associated with the number of reproductives, which indicates that LRE was also operating.
Conclusions
Our results suggest that although the population structure seems to meet the requirements of LRC, the sex ratio is not modulated solely by LRC. Instead, the availability of female helpers at the founding stage likely mitigates the sex ratio predicted by LRC through LRE. Thus, LRC at the founding stage and LRE at the reproductive stage synergistically modulate the colony sex ratio in R. plebeiana.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- FigS1Barplot.eps
Fig. S1. Population assignments from STRUCTURE based on microsatellite data. The figure was drawn at K = 2 for 148 individuals.
- TableS1populations.docx
- TableS2primers.docx
- TableS3GLM.docx
- TableS4.docx
Loading...