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Research article
Reduction of Lateral Shoots by RNA Interference and by Chemical Mutation of Genes Involved in Axillary Meristem Regulation and Field Trials of Mutant Lines in Nicotiana Tabacum L.
Kaori Hamano
Tobacco Institute Inc
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9381-6620
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Lateral branches vigorously proliferate in tobacco after topping of inflorescence portions of stems for maturation of leaves to be harvested. Therefore, tobacco varieties with reduced lateral shoots are highly desired by farmers.
Results
Genetic reduction of lateral shoots was attempted in tobacco. Two groups of genes were examined by RNA interference: homologues of the genes reported as involved in the formation of lateral shoots in other plants, and genes highly expressed in primordial stages of axillary buds. Although "primary" lateral shoots that grew after the plants were topped off when flower buds emerged were not much affected, "secondary" lateral shoots, which appeared from the abaxial sides of the bases of the primary lateral shoots, were suppressed significantly by knock-down of five genes, NtLs, NtBl1, NtREV, VE7, and VE12. Chemical mutation of three of them, NtLs, NtBl1, and NtREV, similarly reduced secondary and "tertiary" lateral shoots but not primary ones. The mutation of NtLs and NtBl1 was backcrossed into an elite variety. The backcross lines were examined for agronomic characteristics in field trials conducted in commercial tobacco production areas. The lines were satisfactory for leaf tobacco production overall and showed good potential as new tobacco varieties.
Conclusion
Suppression of the five genes reduced only secondary and tertiary lateral shoots in tobacco although similar approaches reduced all branches in other plant species. Nevertheless, the mutant lines might greatly relieve farmers because secondary and tertiary lateral shoots are especially cumbersome because they emerge when farmers are burdened by the labor-intensive leaf harvest.
Keywords
chemical mutation, lateral shoot reduction, RNAi, RNAseq, sucker, tobacco
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementalFig.S1.pptx
Supplemental Fig. S1. Seed yield of mutant lines.
- SupplemetalFig.S2.pptx
Supplemental Fig. S2. Number of flowers of Coker319-bl1-1.
- SupplementalTables.docx
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CURRENT STATUS: Under Review
Version 1
Posted 06 Jan, 2021
No community comments so far
Reviewer #2 agreed
On 03 Jan, 2021
Editor assigned
On 03 Jan, 2021
Reviewers invited
Invitations sent on 03 Jan, 2021
Reviewer #1 agreed
On 03 Jan, 2021
Submission checks complete
On 31 Dec, 2020
Editor invited
On 29 Dec, 2020
First submitted
On 20 Dec, 2020
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Reduction of Lateral Shoots by RNA Interference and by Chemical Mutation of Genes Involved in Axillary Meristem Regulation and Field Trials of Mutant Lines in Nicotiana Tabacum L.
Kaori Hamano
Tobacco Institute Inc
Corresponding Author
ORCiD: https://orcid.org/0000-0001-9381-6620
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 06 Jan, 2021
No community comments so far
Reviewer #2 agreed
On 03 Jan, 2021
Editor assigned
On 03 Jan, 2021
Reviewers invited
Invitations sent on 03 Jan, 2021
Reviewer #1 agreed
On 03 Jan, 2021
Submission checks complete
On 31 Dec, 2020
Editor invited
On 29 Dec, 2020
First submitted
On 20 Dec, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Lateral branches vigorously proliferate in tobacco after topping of inflorescence portions of stems for maturation of leaves to be harvested. Therefore, tobacco varieties with reduced lateral shoots are highly desired by farmers.
Results
Genetic reduction of lateral shoots was attempted in tobacco. Two groups of genes were examined by RNA interference: homologues of the genes reported as involved in the formation of lateral shoots in other plants, and genes highly expressed in primordial stages of axillary buds. Although "primary" lateral shoots that grew after the plants were topped off when flower buds emerged were not much affected, "secondary" lateral shoots, which appeared from the abaxial sides of the bases of the primary lateral shoots, were suppressed significantly by knock-down of five genes, NtLs, NtBl1, NtREV, VE7, and VE12. Chemical mutation of three of them, NtLs, NtBl1, and NtREV, similarly reduced secondary and "tertiary" lateral shoots but not primary ones. The mutation of NtLs and NtBl1 was backcrossed into an elite variety. The backcross lines were examined for agronomic characteristics in field trials conducted in commercial tobacco production areas. The lines were satisfactory for leaf tobacco production overall and showed good potential as new tobacco varieties.
Conclusion
Suppression of the five genes reduced only secondary and tertiary lateral shoots in tobacco although similar approaches reduced all branches in other plant species. Nevertheless, the mutant lines might greatly relieve farmers because secondary and tertiary lateral shoots are especially cumbersome because they emerge when farmers are burdened by the labor-intensive leaf harvest.
Figure 1
Figure 2
Figure 3