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Research Article
Yuzhong Zheng
Hanshan Normal University
Corresponding Author
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Background: Saussureae Involucratae Herba, known as “snow lotus” in Uyghur and/or Chinese medicines, is the dried aerial part of Saussurea involucrata (Kar. et Kir.) Sch.-Bip. (Asteraceae). One of the known pharmacological applications of this herb is to suppress chronic inflammation. Nepetin is considered as a bioactive flavonoid of Saussureae Involucratae Herba. Here, we are probing the efficacy of nepetin in modulating lipopolysaccharide (LPS)-stimulated inflammatory responses in cultured human keratinocytes.
Results: In cultured keratinocytes, applied nepetin prevented the LPS-induced cell death. In parallel, the productions of inflammatory mediators, i.e. iNOS, COX-2，PGES2 and NO, were declined after nepetin challenge in LPS-treated keratinocytes. Besides, the treatment of nepetin in LPS-induced cultures suppressed the expressions of cytokines, i.e. IL-1β, IL-6 and TNF-α, in a dose-dependent manner. The productions of inflammatory modulators and/or cytokines could be accounted by nepetin-mediated NF-kB translocation from cytosol to nucleus within the inflammatory activated keratinocytes. In accord to this notion, the formation of ROS, as induced by LPS, could be reduced by nepetin challenge.
Conclusions: The aforementioned results suggested that nepetin could account the anti-inflammatory properties of Saussureae Involucratae Herba, at least during the skin inflammation, e.g. atopic dermatitis.
Keywords
Nepetin, Atopic Dermatitis, Lipopolysaccharide, Inflammatory, Keratinocytes
Figure 1
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This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryFig.pdf
Supplementary Figure 1. Chromatography of nepetin and cell viability [A]: Typical HPLC fingerprint of methanol extract of Saussureae Involucratae Herba (1 mg/mL) and nepetin marker (70 μg/mL) were shown here. Chemical structure of nepetin was inserted. The injection volume was at 10 μL. [B]: Cultured cells were exposed to a series concentration of nepetin for 48 hours. The cell viability was determined by MTT assay. Cell viability is expressed as percentage of control (cells without treatment), Mean ± SEM. *** p < 0.001 as compared to control.
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CURRENT STATUS: Under Revision
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Posted 16 Jan, 2021
No community comments so far
Editorial decision: Major revision
On 26 Mar, 2021
Reviews received
Received 23 Mar, 2021
Reviews received
Received 09 Feb, 2021
Reviewers agreed
On 14 Jan, 2021
Reviewers agreed
On 13 Jan, 2021
Reviewers invited
Invitations sent on 13 Jan, 2021
Editor assigned
On 13 Jan, 2021
Editor invited
On 12 Jan, 2021
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On 12 Jan, 2021
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Integrative & Complementary Medicine
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A new preprint design is coming!
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This preprint is under consideration at BMC Complementary Medicine and Therapies. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Yuzhong Zheng
Hanshan Normal University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 16 Jan, 2021
No community comments so far
Editorial decision: Major revision
On 26 Mar, 2021
Reviews received
Received 23 Mar, 2021
Reviews received
Received 09 Feb, 2021
Reviewers agreed
On 14 Jan, 2021
Reviewers agreed
On 13 Jan, 2021
Reviewers invited
Invitations sent on 13 Jan, 2021
Editor assigned
On 13 Jan, 2021
Editor invited
On 12 Jan, 2021
Submission checks complete
On 12 Jan, 2021
First submitted
On 02 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Integrative & Complementary Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Saussureae Involucratae Herba, known as “snow lotus” in Uyghur and/or Chinese medicines, is the dried aerial part of Saussurea involucrata (Kar. et Kir.) Sch.-Bip. (Asteraceae). One of the known pharmacological applications of this herb is to suppress chronic inflammation. Nepetin is considered as a bioactive flavonoid of Saussureae Involucratae Herba. Here, we are probing the efficacy of nepetin in modulating lipopolysaccharide (LPS)-stimulated inflammatory responses in cultured human keratinocytes.
Results: In cultured keratinocytes, applied nepetin prevented the LPS-induced cell death. In parallel, the productions of inflammatory mediators, i.e. iNOS, COX-2，PGES2 and NO, were declined after nepetin challenge in LPS-treated keratinocytes. Besides, the treatment of nepetin in LPS-induced cultures suppressed the expressions of cytokines, i.e. IL-1β, IL-6 and TNF-α, in a dose-dependent manner. The productions of inflammatory modulators and/or cytokines could be accounted by nepetin-mediated NF-kB translocation from cytosol to nucleus within the inflammatory activated keratinocytes. In accord to this notion, the formation of ROS, as induced by LPS, could be reduced by nepetin challenge.
Conclusions: The aforementioned results suggested that nepetin could account the anti-inflammatory properties of Saussureae Involucratae Herba, at least during the skin inflammation, e.g. atopic dermatitis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryFig.pdf
Supplementary Figure 1. Chromatography of nepetin and cell viability [A]: Typical HPLC fingerprint of methanol extract of Saussureae Involucratae Herba (1 mg/mL) and nepetin marker (70 μg/mL) were shown here. Chemical structure of nepetin was inserted. The injection volume was at 10 μL. [B]: Cultured cells were exposed to a series concentration of nepetin for 48 hours. The cell viability was determined by MTT assay. Cell viability is expressed as percentage of control (cells without treatment), Mean ± SEM. *** p < 0.001 as compared to control.
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