Among families of heterocyclic compounds, imidazoles play an important role because of its bioactivity and sensor properties. Imidazole and its derivatives have attracted increasing interest in the field of chemical research because it acts as a precursor in synthetic reactions towards, primarily for the preparation of functionalized materials. Some of the well-known bio-components of human organisms such as Vitamin B12, amino acid histidine, DNA base structure components, histamine, purines, and biotin contain the imidazole nucleus as the main structure. Several synthetic drug molecules structure that contain the imidazole ring as the main component include cimetidine, azomycin, and metronidazole and also have significant application in the various fields [1, 2].
Metal oxide nanoparticles have gained promising research attention due to its exclusive size-dependent optical and electronic properties. They are also applied in various biotechnological fields such as drug delivery, luminescence tagging, and immunoassay. As a result of recent advances in the field, the interaction between organic molecules and the surfaces of the semiconductor materials is an interestingly booming area of research [3 − 6]. An organic molecule on surfaces of the semiconductor leads to the enhanced interaction of the semiconductor with an incident electromagnetic wave [7, 8]. TiO2 have been applied in the development of various technologies due to their excellent stability, low cost, non-toxicity, and physicochemical property. TiO2 are generally used to improve their performance in many end-use applications. Reportedly, ethanol suspension of Au/TiO2, maintaining charge equilibrium by transferring photoexcited electrons from TiO2 to Au nanoparticles . Because of the usage of ethanol as a solvent, possible recombination of electrons in Au and holes in TiO2 has not been studied [11–15]. Various studies have been developed for the quenching of the photoluminescence because of the charge from semiconductor nanoparticles [16–20]. The highest occupied molecular orbital and lowest unoccupied molecular orbital potentials for the designed sensor must match with the conduction and valence band edges of the semiconductor TiO2 nanoparticles. In this contribution, we report fluorescence enhancement by TiO2 nanoparticles by 5-amino-2-mercaptobenzimidazole (fluorochrome). The observed fluorescence enhancement is unique to study the interaction between virgin and Au loaded TiO2 nanoparticles with fluorochrome.