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Research Article
Expression Profiling of the Dof Gene Family Under Abiotic Stresses in Spinach
Ray Ming
Department of Plant Biology, University of Illinois at Urbana-Champaign
Corresponding Author
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DNA-binding with one finger (Dof) transcription factors are plant-specific transcription factors involved in numerous pathways of plant development, especially in respond to abiotic stresses. Although genome-wide analysis of this gene family has been performed in many species, Dof genes in spinach have not been thoroughly analyzed. We performed a genome-wide analysis and characterization of Dof gene family in spinach. Twenty two Dof genes were identified and classified into four groups with nine subgroups, which was further corroborated by gene structure and motif analyses. Ka/Ks analysis revealed that SoDofs were subjected to purify selection. Using Cis-acting elements analysis, SoDofs were involved in plant growth and development, plant hormones and stress responses. Expression profiling demonstrated that SoDofs expressed in leaf and inflorescence, and responded to cold, heat, and drought stresses. SoDof22 expressed the highest level in male flowers and under cold stress. These results provided a genome-wide analysis of SoDof genes, their gender- and tissue-specific expression, and response to abiotic stresses. The knowledge and resources gained from these analyses will benefit spinach improvement.
Keywords
Dof transcription factors, gene expression analysis, Spinach, tissue-specific expression
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This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryData.xlsx
Data.S1 The quantity of cis-elements for each SoDofs. The color scheme used to present number is sky-blue/red: light-yellow boxes indicate fewer cis-elements, sky-blue indicate none, and red indicate a fold number in relation to mean value.
- Supplementarytable.docx
Table S1. Ka/Ks analysis and estimated divergence time for SoDofs pairs Table S2. qRT-PCR primers used in this study.
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Expression Profiling of the Dof Gene Family Under Abiotic Stresses in Spinach
Ray Ming
Department of Plant Biology, University of Illinois at Urbana-Champaign
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 08 Jan, 2021
No community comments so far
Review #1 received
Received 17 Jan, 2021
Review #2 received
Received 17 Jan, 2021
Reviewer #2 agreed
On 13 Jan, 2021
Reviewer #6 agreed
On 13 Jan, 2021
Reviewer #9 agreed
On 13 Jan, 2021
Reviewer #10 agreed
On 13 Jan, 2021
Reviewer #3 agreed
On 13 Jan, 2021
Reviewer #8 agreed
On 13 Jan, 2021
Reviewer #5 agreed
On 13 Jan, 2021
Reviewer #1 agreed
On 13 Jan, 2021
Reviewer #7 agreed
On 13 Jan, 2021
Reviewer #4 agreed
On 13 Jan, 2021
Reviewers invited
Invitations sent on 13 Jan, 2021
Editor assigned
On 13 Jan, 2021
Editor invited
On 07 Jan, 2021
Submission checks complete
On 06 Jan, 2021
First submitted
On 04 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
DNA-binding with one finger (Dof) transcription factors are plant-specific transcription factors involved in numerous pathways of plant development, especially in respond to abiotic stresses. Although genome-wide analysis of this gene family has been performed in many species, Dof genes in spinach have not been thoroughly analyzed. We performed a genome-wide analysis and characterization of Dof gene family in spinach. Twenty two Dof genes were identified and classified into four groups with nine subgroups, which was further corroborated by gene structure and motif analyses. Ka/Ks analysis revealed that SoDofs were subjected to purify selection. Using Cis-acting elements analysis, SoDofs were involved in plant growth and development, plant hormones and stress responses. Expression profiling demonstrated that SoDofs expressed in leaf and inflorescence, and responded to cold, heat, and drought stresses. SoDof22 expressed the highest level in male flowers and under cold stress. These results provided a genome-wide analysis of SoDof genes, their gender- and tissue-specific expression, and response to abiotic stresses. The knowledge and resources gained from these analyses will benefit spinach improvement.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8