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Research Article
Detection of SARS-COV-2 Antibodies in Pediatric Kidney Transplant Patients
Alanoud Alshami
King Fahad Specialist Hospital
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The seroprevalance of SARS-COV-2 infection have been studied in immunocompetent children. However, data in pediatric Kidney Transplant population (PKT) is lacking.
Methods
We screened 72 PKT patients who came for routine blood work in the outpatient clinic using two commercial immunoassays that measures IgG antibodies against SARS-COV-2 spike protein and IgG against the Nucleocapsid protein. Majority of patients with positive serology had their serology test repeated at least once during subsequent clinic follow up. Patients were confirmed to have SARS-COV-2 infection if they only have both tests positive.
Results
Eight patients out of the 72 screened (11.1%) had positive SARS-COV-2 IgG antibodies in both serological tests. Of those tested positive, 4 had positive SARS-COV-2 PCR before screening. All patients were asymptomatic or had history of mild symptoms. All tested patient had persistently positive antibodies at median follow-up time of 75 days (IQR, 44.5, 86.5). One patient remained to have positive PCR at 75 days and positive serology test at 120 days post infection.
conclusion
The Serooprevalence of SARS-COV-2 was relatively high (11.1%) in our population. SARS-COV-2 PCR seems to remain positive in PKT population for longer duration than general population. Although, all patients were asymptomatic or mildly symptomatic, they mounted a strong humoral immune response that persisted for few months despite being on triple immunosuppressants. These findings will have positive implications on vaccination efficacy in this group.
Keywords
SARS-COV-2 infection, PKT, SARS-COV-2 PCR, CVOID-19
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CURRENT STATUS: Under Review
Version 1
Posted 12 Jan, 2021
No community comments so far
Review #1 received
Received 17 Jan, 2021
Review #2 received
Received 17 Jan, 2021
Reviewer #1 agreed
On 13 Jan, 2021
Reviewer #4 agreed
On 13 Jan, 2021
Reviewer #3 agreed
On 13 Jan, 2021
Reviewer #5 agreed
On 13 Jan, 2021
Reviewer #2 agreed
On 13 Jan, 2021
Reviewer #6 agreed
On 13 Jan, 2021
Reviewers invited
Invitations sent on 13 Jan, 2021
Editor assigned
On 13 Jan, 2021
Editor invited
On 07 Jan, 2021
Submission checks complete
On 07 Jan, 2021
First submitted
On 05 Jan, 2021
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Subject Areas
Urology & Nephrology
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This preprint is under consideration at BMC Nephrology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Detection of SARS-COV-2 Antibodies in Pediatric Kidney Transplant Patients
Alanoud Alshami
King Fahad Specialist Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 Jan, 2021
No community comments so far
Review #1 received
Received 17 Jan, 2021
Review #2 received
Received 17 Jan, 2021
Reviewer #1 agreed
On 13 Jan, 2021
Reviewer #4 agreed
On 13 Jan, 2021
Reviewer #3 agreed
On 13 Jan, 2021
Reviewer #5 agreed
On 13 Jan, 2021
Reviewer #2 agreed
On 13 Jan, 2021
Reviewer #6 agreed
On 13 Jan, 2021
Reviewers invited
Invitations sent on 13 Jan, 2021
Editor assigned
On 13 Jan, 2021
Editor invited
On 07 Jan, 2021
Submission checks complete
On 07 Jan, 2021
First submitted
On 05 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Urology & Nephrology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The seroprevalance of SARS-COV-2 infection have been studied in immunocompetent children. However, data in pediatric Kidney Transplant population (PKT) is lacking.
Methods
We screened 72 PKT patients who came for routine blood work in the outpatient clinic using two commercial immunoassays that measures IgG antibodies against SARS-COV-2 spike protein and IgG against the Nucleocapsid protein. Majority of patients with positive serology had their serology test repeated at least once during subsequent clinic follow up. Patients were confirmed to have SARS-COV-2 infection if they only have both tests positive.
Results
Eight patients out of the 72 screened (11.1%) had positive SARS-COV-2 IgG antibodies in both serological tests. Of those tested positive, 4 had positive SARS-COV-2 PCR before screening. All patients were asymptomatic or had history of mild symptoms. All tested patient had persistently positive antibodies at median follow-up time of 75 days (IQR, 44.5, 86.5). One patient remained to have positive PCR at 75 days and positive serology test at 120 days post infection.
conclusion
The Serooprevalence of SARS-COV-2 was relatively high (11.1%) in our population. SARS-COV-2 PCR seems to remain positive in PKT population for longer duration than general population. Although, all patients were asymptomatic or mildly symptomatic, they mounted a strong humoral immune response that persisted for few months despite being on triple immunosuppressants. These findings will have positive implications on vaccination efficacy in this group.