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Calcium Sulfate Versus PMMA in One Stage Repair of Critical-sized Femoral Defects: A Vitro Study
Hanjun Qin
Southern Medical University Nanfang Hospital
Corresponding Author
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Polymethylmethacrylate (PMMA) cement has been used in Masquelet technique to repair bone defects, but it needs additional surgery like bone grafting. This study was to compare the efficacy between PMMA and calcium sulfate (CS) used as a spacer in one-stage reconstruction of critical-sized segmental bone defects. Sprague Dawley rats (n=30) were used to create models of critical-sized femoral defect (5mm) which were then randomized into CS, PMMA and control groups equally. the bone defects were filled with CS, PMMA or nothing. Gross observation, digital radiography, histological observation and micro-computed tomography were conducted at week 20 after surgery for comparison between the three groups. The expression of Osterix, Nestin and Osteocalcin at the bone defects was also detected by immunofluorescent staining. The results of X-Ray and μCT showed that new bone formation in CS group was superior to that in PMMA group. H&E, Safranin O & fast green and Masson staining showed obvious endochondral ossification and fibrocartilage tissues at the ends of bone defects in CS group. Much more new bone tissue formed near the ends of bone defects in CS group. In addition, osteogenic and angiogenic activities were significantly increased in CS and PMMA groups. Compared with PMMA and control groups, the expression of Osteoblast-associated protein at the bone defects in CS group was higher. In general, due to the degradability of CS and superior osteogenesis ability, CS may replace PMMA as a new filling material in clinical application.
Keywords
Induced membrane, calcium sulfate, PMMA, bone defect, Masquelet technique
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This preprint is under consideration at Journal of Translational Medicine. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Calcium Sulfate Versus PMMA in One Stage Repair of Critical-sized Femoral Defects: A Vitro Study
Hanjun Qin
Southern Medical University Nanfang Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 13 Jan, 2021
No community comments so far
Editor assigned
On 06 Jan, 2021
Submission checks complete
On 06 Jan, 2021
Editor invited
On 06 Jan, 2021
First submitted
On 05 Jan, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Translational Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Polymethylmethacrylate (PMMA) cement has been used in Masquelet technique to repair bone defects, but it needs additional surgery like bone grafting. This study was to compare the efficacy between PMMA and calcium sulfate (CS) used as a spacer in one-stage reconstruction of critical-sized segmental bone defects. Sprague Dawley rats (n=30) were used to create models of critical-sized femoral defect (5mm) which were then randomized into CS, PMMA and control groups equally. the bone defects were filled with CS, PMMA or nothing. Gross observation, digital radiography, histological observation and micro-computed tomography were conducted at week 20 after surgery for comparison between the three groups. The expression of Osterix, Nestin and Osteocalcin at the bone defects was also detected by immunofluorescent staining. The results of X-Ray and μCT showed that new bone formation in CS group was superior to that in PMMA group. H&E, Safranin O & fast green and Masson staining showed obvious endochondral ossification and fibrocartilage tissues at the ends of bone defects in CS group. Much more new bone tissue formed near the ends of bone defects in CS group. In addition, osteogenic and angiogenic activities were significantly increased in CS and PMMA groups. Compared with PMMA and control groups, the expression of Osteoblast-associated protein at the bone defects in CS group was higher. In general, due to the degradability of CS and superior osteogenesis ability, CS may replace PMMA as a new filling material in clinical application.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9