Clinical diagnosis of human chronic myeloid leukemia (CML)
In the present study, we included the peripheral blood of T-ALL patients. The morphology of blast crisis cells from peripheral blood smears, aspirates from the smears of bone marrow, and bone marrow trephine biopsy specimens, along with immunophenotypic findings and flow cytometry, are shown in Fig. 1 (a, b), which shows that blast cells were stained with Leishman dye. Furthermore, flow cytometry results helped identify phenotypes with different biomarkers, such as CD34, CD33, CD14, CD20, CD10, CD19, HLA-DR, TdT, CD2, CD3, CD5, CD7, CD13, CD19, CD20, CD23, CD45, CD64, CD79a, CD117, and CD 200. Blast cells were gated on CD45 vs side scatter. The expression of myeloid markers (CD13, CD33, CD117, CD14, CD64, cMPO), B-lymphoid markers (CD19, CD20, CD79a, CD10), T-lymphoid markers (CD3, cytoplasmic CD3, CD2, CD5, CD7) and immaturity markers (CD34, TdT, HLA-DR) was studied. We performed flow cytometry analysis of different patients, as shown in Fig. 1 (c-e). The results are summarized in Table I, and the extension is shown in Table S3. Ninety percent of blast cells gated on dim CD45 and extended to the monocytic region on CD45 versus the side scatter plot, as shown in Fig. 1 (g). A reliable number of CD45 events (20,000) were measured, and we found that 95% of patients had approximately 15,000 blast cells in 20000 events. Flow cytometry data confirmed the presence of blast cells in CML patients.
The expression levels of serum IL-8 were elevated in all patients (19 ± 2.3, p < 0.0001) with T-ALL compared to healthy individuals (4.8 ± 2.2). IL-8 levels at the genomic and proteomic levels in T-ALL (RQ 7.8 ± 1.2 p < 0.0001) patients were higher in different histopathological subgroups than in healthy individuals (1.542 ± 03342). Analysis of the ROC curve (Fig. 2) indicated that both IL-8 showed good discriminative efficiency among the healthy volunteers and the total patients with leukemia/T-ALL (IL-8: AUC-0.93553, p = 5.0815e-9). Moreover, the ROC curves suggested that IL-8 possesses good sensitivity and specificity to differentiate healthy volunteers from T-ALL patients.