SDS-PAGE Profile of Total Soluble Proteins
The protein pattern of T. propinqua seedlings in one-dimensional polyacrylamide gel electrophoresis (SDS-PAGE) showed no remarkable differences (Fig. 1). The relative expression of 18 protein bands (with molecular weights of about 102.3, 93.8, 71.4, 66, 55, 53.2, 47.1, 41.3, 40.2, 38.4, 35.1, 32, 26.8, 25, 10.9, 10.5, 10, 9.4 KD) appeared under the control and heat treatments. The relative intensity of these bands were often downregulated after 4 h and 48 h at high temperature, while upregulated after 24 h of high temperature. The expression of protein band (with molecular weights of about 86.5 and 30.2 KD) newly appeared at 24 h of high temperature. On the other hand, protein band of 31.4 KD appeared at 48 h under normal conditions then disappeared under high temperature of 48 h.
Heat Stress-Responsive Proteome in T. propinqua
In total, 931 protein species and 81 gene at germinating stage were identified. After 4 hours of germination, 110 protein were identified in C4 (control plants) and 173 protein scored in T4 (treated plants). However, after 24 h, 181 protein were identified in C24 (control) decreased to 151 protein in T24 (treated). After 48 h, 136 protein were identified in C48 (control) increased to 180 protein in T48 (treated). Eighty-one genes were identified in control and treated T. propinqua seedlings depended on appearance and disappearance of proteins they code for (Table 1).
Table 1
List of expressed genes by heat stress in T. propinqua seedlings depending on appearance and disappearance under heat stress treatments using MALDI-Triple-TOF.
No.
|
Gene
|
Description
|
C4
|
T4
|
C24
|
T24
|
C48
|
T48
|
1.
|
rpoA
|
DNA-directed RNA polymerase subunit alpha
|
1
|
1
|
1
|
1
|
1
|
1
|
2.
|
rpoC1
|
DNA-directed RNA polymerase subunit beta'
|
1
|
1
|
1
|
1
|
1
|
1
|
3.
|
rpoC2
|
DNA-directed RNA polymerase subunit beta''
|
1
|
1
|
1
|
1
|
1
|
1
|
4.
|
rpoB
|
DNA-directed RNA polymerase subunit beta
|
1
|
1
|
1
|
1
|
1
|
1
|
5.
|
rpb2
|
DNA-directed RNA polymerase subunit B
|
1
|
0
|
0
|
0
|
1
|
1
|
6.
|
WRKY21
|
WRKY transcription factor 21
|
1
|
1
|
1
|
1
|
1
|
1
|
7.
|
matK
|
Maturase K
|
1
|
1
|
1
|
1
|
1
|
1
|
8.
|
matR
|
Maturase
|
1
|
1
|
1
|
1
|
1
|
1
|
9.
|
EMB2765
|
Embryo defective 2765
|
1
|
1
|
1
|
1
|
0
|
1
|
10.
|
PHYC
|
Phytochrome C
|
1
|
1
|
1
|
0
|
0
|
1
|
11.
|
rpl14
|
50S ribosomal protein L14, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
12.
|
rpl23
|
50S ribosomal protein L23, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
13.
|
rpl22
|
50S ribosomal protein L22, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
14.
|
rpl20
|
50S ribosomal protein L20, chloroplastic
|
1
|
0
|
0
|
0
|
0
|
0
|
15.
|
rpl2
|
50S ribosomal protein L2, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
0
|
16.
|
rpl16
|
50S ribosomal protein L16, chloroplastic
|
0
|
1
|
1
|
0
|
0
|
1
|
17.
|
rpl36
|
50S ribosomal protein L36, chloroplastic
|
0
|
0
|
1
|
0
|
0
|
0
|
18.
|
rpl32
|
50S ribosomal protein L32, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
19.
|
rpl33
|
50S ribosomal protein L33, chloroplastic
|
0
|
1
|
0
|
0
|
0
|
1
|
20.
|
rps18
|
30S ribosomal protein S18, chloroplastic
|
0
|
1
|
1
|
0
|
1
|
1
|
21.
|
rps8
|
30S ribosomal protein S8, chloroplastic
|
1
|
1
|
1
|
0
|
1
|
1
|
22.
|
rps4
|
30S ribosomal protein S4, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
23.
|
rps19
|
30S ribosomal protein S19, chloroplastic
|
1
|
0
|
0
|
1
|
1
|
0
|
24.
|
rps7
|
30S ribosomal protein S7, chloroplastic
|
1
|
0
|
1
|
1
|
0
|
0
|
25.
|
rps11
|
30S ribosomal protein S11, chloroplastic
|
0
|
1
|
1
|
1
|
1
|
1
|
26.
|
rps14
|
30S ribosomal protein S14, chloroplastic
|
0
|
0
|
1
|
0
|
0
|
0
|
27.
|
rps2
|
30S ribosomal protein S2, chloroplastic
|
1
|
1
|
1
|
1
|
0
|
1
|
28.
|
rps15
|
30S ribosomal protein S15, chloroplastic
|
1
|
0
|
1
|
1
|
1
|
1
|
29.
|
rps3
|
Ribosomal protein S3
|
1
|
1
|
1
|
1
|
1
|
1
|
30.
|
petA
|
Cytochrome f
|
1
|
1
|
1
|
1
|
1
|
1
|
31.
|
petD
|
Cytochrome b6-f complex subunit 4
|
1
|
1
|
1
|
1
|
1
|
1
|
32.
|
psbE
|
Cytochrome b559 subunit alpha
|
1
|
0
|
1
|
0
|
1
|
1
|
33.
|
ycf3
|
Photosystem I assembly protein Ycf3
|
0
|
0
|
0
|
0
|
1
|
0
|
34.
|
ycf4
|
Photosystem I assembly protein Ycf4
|
0
|
1
|
1
|
0
|
0
|
1
|
35.
|
psbL
|
Photosystem II reaction center protein L
|
1
|
1
|
1
|
1
|
1
|
1
|
36.
|
psbI
|
Photosystem II reaction center protein I
|
1
|
1
|
1
|
1
|
1
|
1
|
37.
|
psbK
|
Photosystem II reaction center protein K
|
1
|
1
|
0
|
0
|
1
|
0
|
38.
|
psaA
|
Photosystem I P700 chlorophyll a apoprotein A1
|
0
|
0
|
0
|
0
|
0
|
1
|
39.
|
psaB
|
Photosystem I P700 chlorophyll a apoprotein A2
|
1
|
1
|
1
|
1
|
1
|
1
|
40.
|
psaC
|
Photosystem I iron-sulfur center
|
1
|
0
|
1
|
0
|
1
|
1
|
41.
|
psbB
|
Photosystem II CP47 reaction center protein
|
0
|
0
|
0
|
0
|
1
|
0
|
42.
|
psbH
|
Photosystem II reaction center protein H
|
1
|
0
|
0
|
1
|
1
|
0
|
43.
|
psbD
|
Photosystem II D2 protein
|
0
|
0
|
1
|
0
|
0
|
0
|
44.
|
RCA1
|
Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
45.
|
RCA2
|
Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic
|
1
|
1
|
0
|
1
|
1
|
1
|
46.
|
rbcL
|
Ribulose bisphosphate carboxylase large chain
|
1
|
1
|
1
|
1
|
1
|
1
|
47.
|
ycf2
|
Hypothetical chloroplast RF21
|
1
|
1
|
1
|
1
|
1
|
1
|
48.
|
atpE
|
ATP synthase epsilon chain, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
49.
|
atp1
|
ATP synthase subunit alpha
|
1
|
1
|
1
|
1
|
1
|
1
|
50.
|
atpB
|
ATP synthase subunit beta, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
51.
|
atpA
|
ATP synthase subunit alpha, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
52.
|
atpI
|
ATP synthase subunit a, chloroplastic
|
1
|
1
|
1
|
1
|
0
|
0
|
53.
|
atpF
|
ATP synthase subunit b, chloroplastic
|
1
|
1
|
1
|
0
|
1
|
1
|
54.
|
ndhE
|
NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
55.
|
ndhD
|
NAD(P)H-quinone oxidoreductase chain 4, chloroplastic
|
0
|
0
|
0
|
1
|
0
|
1
|
56.
|
ndhB
|
NADH-quinone oxidoreductase subunit N
|
0
|
1
|
1
|
0
|
1
|
1
|
57.
|
ndhF
|
NAD(P)H-quinone oxidoreductase subunit 5, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
58.
|
ndhA
|
NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
59.
|
ndhJ
|
NAD(P)H-quinone oxidoreductase subunit J, chloroplastic
|
1
|
1
|
1
|
0
|
1
|
1
|
60.
|
ndhH
|
NAD(P)H-quinone oxidoreductase subunit H, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
61.
|
ndhI
|
NAD(P)H-quinone oxidoreductase subunit I, chloroplastic
|
0
|
0
|
0
|
1
|
0
|
1
|
62.
|
ndhK
|
NAD(P)H-quinone oxidoreductase subunit K, chloroplastic
|
1
|
1
|
0
|
0
|
1
|
1
|
63.
|
ycf1
|
Protein TIC 214
|
1
|
1
|
1
|
1
|
1
|
1
|
64.
|
cemA
|
envelope membrane protein, chloroplastic
|
0
|
1
|
1
|
1
|
0
|
1
|
65.
|
AKT1
|
Potassium channel
|
1
|
1
|
1
|
1
|
0
|
1
|
66.
|
NHX
|
Vacuolar Na+/H + antiporter
|
1
|
1
|
1
|
1
|
1
|
1
|
67.
|
DGAT1a
|
O-acyltransferase
|
0
|
0
|
1
|
0
|
0
|
0
|
68.
|
DGAT1b
|
O-acyltransferase
|
0
|
1
|
0
|
0
|
0
|
0
|
69.
|
CAAT1
|
Cinnamyl alcohol acyltransferase 1
|
0
|
0
|
0
|
0
|
0
|
1
|
70.
|
CAAT2
|
Cinnamyl alcohol acyltransferase 2
|
1
|
0
|
1
|
1
|
0
|
1
|
71.
|
accD
|
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic
|
1
|
1
|
1
|
1
|
1
|
1
|
72.
|
ccsA
|
Cytochrome c biogenesis protein CcsA
|
1
|
1
|
1
|
0
|
1
|
1
|
73.
|
ccmB
|
Cytochrome c biogenesis
|
0
|
0
|
1
|
1
|
0
|
1
|
74.
|
VP1-1
|
Vacuolar H+-pyrophosphatase
|
0
|
0
|
1
|
0
|
0
|
1
|
75.
|
VP1-2
|
Vacuolar H+-pyrophosphatase
|
0
|
0
|
1
|
0
|
0
|
0
|
76.
|
clpP
|
ATP-dependent Clp protease proteolytic subunit
|
1
|
1
|
1
|
1
|
0
|
1
|
77.
|
Xdh
|
Xanthine dehydrogenase
|
1
|
1
|
1
|
1
|
1
|
1
|
78.
|
APS1
|
Allylphenol synthase 1
|
0
|
0
|
0
|
1
|
0
|
1
|
79.
|
APS2
|
Allylphenol synthase 2
|
0
|
0
|
1
|
1
|
1
|
1
|
80.
|
PPS1
|
Propenylphenol synthase 1
|
1
|
1
|
0
|
1
|
1
|
1
|
81.
|
psbA
|
PsbA
|
0
|
0
|
1
|
1
|
1
|
0
|
The genes of proteins were classified by gene ontology (GO) annotation, and then classified into three functional groups: molecular function, biological process, and cellular component. The results of the GO analyses for the various treatments are shown in (Table 1, Fig. 2, 3). Ten genes are involved in transcription, encoding rpoA, rpoC1, rpoC2, rpoB, rpb2, WRKY21, matK, matR, MB2765 and PHYC, among them rpb2 and PHYC disappeared after 4 and 24 h of heat treatment, but EMB2765 and PHYC appeared after 48 h under 40oC heat stress. 19 genes for rpl14, rpl23, rpl22, rpl20, rpl2, rpl16, rpl36, rpl32, rpl33, rps18, rps8, rps4, rps19, rps7, rps11, rps14, rps2, rps15 and rps3 are involved in translation (Figs. 2). Among them, some genes for rpl and rps disappeared such as rpl20, rpl2, rpl36, rps8, rps7, rps14, rps15, however, other genes were observed like rpl33, rpl16, rps11, rps2 under different exposure time of heat stress treatments. However, some genes observed after 4 h and 48 h and disappeared after 24 h for example the rpl16 and rps18, but rps19 disappeared after 4 h and 48 h and appeared after 24 h under 40oC of heat stress in T. propinqua seedlings (Table 1).
In T. propinqua seedlings, 15 genes are involved in the transport process (Table l, Fig. 2), among them, ycf1 is involved in protein transport, but cemA is involved in hydrogen ion transport, AKT1 involved in regulation of ion transmembrane transport, and NHX involved in regulation of pH. DGAT1a and DGAT1b involved triglyceride biosynthetic process. CAAT1 and CAAT2 involved in acyltransferase. accD involved in fatty acid biosynthetic process and malonyl-CoA biosynthetic process. ccsA and ccmB involved in cytochrome complex assembly. VP1-1, VP1-2 and clpP involved in hydrolase. Xdh involved in oxidation-reduction process. After 4 h of heat stress, cemA, DGAT1b observed, but CAAT2 disappeared. At 24 h of heat stress, DGAT1a, ccsA, VP1-2, VP1-1 disappeared. At 48 h of heat stress, cemA, AKT1, CAAT1, ccmB, VP1-1, clpP and CAAT2 appeared under 40oC with compared control. APS1, APS2, PPS1 and psbA genes were unknown function. Among them, APS1 appeared at 24 and 48 h, and PPS1 observed at 24 h, however, psbA disappeared after 48 h under 40oC of heat stress compared control. The cellular components of proteins were in chloroplast, nucleus, mitochondrion, membrane, endoplasmic reticulum, ribosome and unknown components (Fig. 3).
On the other hand, 17 genes for petA, petD, psbE, ycf3, ycf4, psbL, psbI, psbK, psaA, psaB, psaC, psbB, psbH, psbD, RCA1, RCA2 and rbcL are involved in photosynthesis and photorespiration (Table 1; Fig. 2). The proteins encoded by some genes disappeared after 4 h under 40oC of heat stress like psbE, psaC, psbH, but the expression of other genes appeared such as ycf4. After 24 h of heat stress, the expression of the psbE, ycf4, psaC and psbD genes disappeared, but psbH and RCA2 observed compared to control. At 48 h of heat stress, ycf3, psbK, psaB and psbH genes disappeared, but the protein encoded by ycf4 and psaA appeared in T. propinqua seedlings. In T. propinqua seedlings, the ycf2, atpE, atp1, atpB, atpA, atpI, atpF, ndhE, ndhD, ndhB, ndhF, ndhA, ndhJ, ndhH, ndhI and ndhK are involved in metabolic process such as ATP synthesis, coupled proton transport, photosynthesis and light reaction (Fig. 2). Among them, the proteins encoded by atpF and ndhJ genes disappeared after 24 h of heat stress; however, the proteins encoded by ndhD and ndhI genes appeared after 24 h, 48 h under 40oC of heat stress compared to the control. On the other hand, the protein encoded by the ndhB gene was observed at 4 h then disappeared after 24 h 40oC of heat stress compared to control (Table 1, Fig. 2). A PCA-biplot of the 81 gene differentially expressed in T. propinqua seedlings under heat stress is shown in Fig. 4.
Top 59-proteins of T. propinqua seedlings differentially expressed in control and heat exposed seedlings based on abundance (NSAF; protein expression) (Table 2, Fig. 5). Four proteins of DNA-directed RNA polymerase such as A0A4Y5PRE0, A0A4Y5PPR0, A0A0M3TGK1 and D3WDG9 decreased under 4 h and 48 h of heat stress; however, A0A3G4R1Z0 increased after 24 h of heat stress compared with control. The protein expression of T1Q0S2 (Maturase K) decreased after 4 h and 24 h but increased after 48 h of heat stress in T. propinqua seedlings. Three of putative LOV domain-containing proteins were A0A126 × 286, A0A140F7J3 and A0A140F7J1 decreased under 4 h of heat stress compared with control. The protein expression decreased after 24 h of heat stress for 20 of ribulose bisphosphate carboxylase proteins i.e. A0A0F6RA63, O20282, Q0ZQ55, O20271, B3FD36, A0A0F6TN85, A0A3G1ZJ92, B3FD32, A0A2U8T527, A9XUT7, B3FD26, A0A0F6RA65, O20257, A0A4Y5PPU8, A0A4Y5PQN6, Q9SBY1, Q9SC93, Q9SC53, Q9SBY0 and Q9SBW6. However, 16 protein increased after 24 h of heat stress; these are Q9BAD9, B3FD25, Q9TI23, Q9TI22, A0A0F6TMP4, A0A3G3NI82, A0A172C083, A0A142LSY1, O20289, O20239, A0A1U9YH05, A0A3G4R1V6, A0A0M3TGJ0, Q9SC24, A0A1S6WN95 and A0A411GWE8. After 48 h of heat stress, the protein expression increased for 27 proteins were A0A0F6RA63, A0A2Z4N431, O20271, B3FD25, O20400, A0A291L1Z1, A0A3G1ZJ92, B3FD32, A0A0F6TMP4, B3FD26, A0A0F6RA65, Q9TI21, O20289, A0A3G3NI75, Q9THY9, B3FD28, Q9BAD8, A0A3G3NI71, A0A4Y5PPU8, A0A3G4R1V6, Q9SBY1, Q9SC24, Q9SC93, Q9SBW8, Q9SBY0, Q9SBX9 and Q9SBW7. However, it decreased in photosystem I assembly protein Ycf3 (A0A4Y5PPR9) after 4 h of heat stress compared to control. One actin protein (A7LHF2) increased after 4 h of heat stress in T. propinqua seedlings.
Table 2
List of top 59 differentially expressed proteins by heat stress in T. propinqua seedlings based on abundance (NSAF), using MALDI-Triple-TOF analysis.
No.
|
Accession
(Uni Prot KB)
|
Description
|
Gene
|
NSAF (Abundance)
|
C4
|
T4
|
C24
|
T24
|
C48
|
T48
|
1
|
A0A4Y5PRE0
|
DNA-directed RNA polymerase subunit beta' (A0A4Y5PRE0_LARTR)
|
rpoC1
|
0.00065
|
0
|
0
|
0
|
0.00027
|
0
|
2
|
A0A3G4R1Z0
|
~
|
rpoC2
|
0
|
0
|
0
|
0.00166
|
0
|
0
|
3
|
A0A4Y5PPR0
|
~
|
rpoC1
|
0.00065
|
0
|
0
|
0
|
0.00027
|
0
|
4
|
A0A0M3TGK1
|
~
|
rpoC1
|
0.00065
|
0
|
0
|
0
|
0.00027
|
0
|
5
|
D3WDG9
|
~
|
rpoC1
|
0.00065
|
0
|
0
|
0
|
0.00027
|
0
|
6
|
T1Q0S2
|
Maturase K (Fragment) (T1Q0S2_9ROSI)
|
matK
|
0.00784
|
0
|
0.00784
|
0.00392
|
0
|
0.00784
|
7
|
A0A126 × 286
|
Putative LOV domain-containing protein (A0A126 × 286_LARTR)
|
N/A
|
0.00036
|
0
|
0
|
0
|
0
|
0
|
8
|
A0A140F7J3
|
~
|
N/A
|
0.00032
|
0
|
0
|
0
|
0
|
0
|
9
|
A0A140F7J1
|
~
|
N/A
|
0.00041
|
0
|
0
|
0
|
0
|
0
|
10
|
A0A0F6RA63
|
Ribulose bisphosphate carboxylase large chain (Fragment) (A0A0F6RA63_9ROSI)
|
rbcL
|
0
|
0
|
0.00015
|
0
|
0
|
0.00011
|
11
|
O20282
|
~
|
~
|
0
|
0
|
0.00016
|
0.00011
|
0
|
0
|
12
|
Q0ZQ55
|
~
|
~
|
0
|
0
|
0.00016
|
0.00011
|
0
|
0
|
13
|
Q9BAD9
|
~
|
~
|
0
|
0
|
0
|
0.00012
|
0
|
0
|
14
|
A0A2Z4N431
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00014
|
15
|
O20271
|
~
|
~
|
0
|
0
|
0.00016
|
0.00011
|
0
|
0.00012
|
16
|
B3FD25
|
~
|
~
|
0
|
0
|
0
|
0.00011
|
0
|
0.00012
|
17
|
B3FD36
|
~
|
~
|
0
|
0
|
0.00016
|
0.00011
|
0
|
0
|
18
|
O20400
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00012
|
19
|
Q9TI23
|
~
|
~
|
0
|
0
|
0
|
0.00011
|
0
|
0
|
20
|
A0A0F6TN85
|
~
|
~
|
0
|
0
|
0.00015
|
0
|
0
|
0
|
21
|
A0A291L1Z1
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00013
|
22
|
Q9TI22
|
~
|
~
|
0
|
0
|
0
|
0.00012
|
0
|
0
|
23
|
A0A3G1ZJ92
|
~
|
~
|
0
|
0
|
0.00016
|
0
|
0
|
0.00012
|
24
|
B3FD32
|
~
|
~
|
0
|
0
|
0.00016
|
0.00010
|
0
|
0.00012
|
25
|
A0A2U8T527
|
~
|
N/A
|
0
|
0
|
0.00015
|
0.00012
|
0
|
0
|
26
|
A9XUT7
|
~
|
rbcL
|
0
|
0
|
0.00015
|
0
|
0
|
0
|
27
|
A0A0F6TMP4
|
~
|
~
|
0
|
0
|
0
|
0.00011
|
0
|
0.00012
|
28
|
B3FD26
|
~
|
~
|
0
|
0
|
0.00016
|
0.00011
|
0
|
0.00012
|
29
|
A0A3G3NI82
|
~
|
~
|
0
|
0
|
0
|
0.00011
|
0
|
0
|
30
|
A0A0F6RA65
|
~
|
~
|
0
|
0
|
0.00015
|
0.00011
|
0
|
0.00011
|
31
|
Q9TI21
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00012
|
32
|
O20257
|
~
|
~
|
0
|
0
|
0.00016
|
0
|
0
|
0
|
33
|
A0A172C083
|
~
|
~
|
0
|
0
|
0
|
0.00034
|
0
|
0
|
34
|
A0A142LSY1
|
~
|
~
|
0
|
0
|
0
|
0.00036
|
0
|
0
|
35
|
O20289
|
~
|
~
|
0
|
0
|
0
|
0.00012
|
0
|
0.00012
|
36
|
O20239
|
~
|
~
|
0
|
0
|
0
|
0.00012
|
0
|
0
|
37
|
A0A1U9YH05
|
~
|
~
|
0
|
0
|
0
|
0.00039
|
0
|
0
|
38
|
A0A3G3NI75
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00012
|
39
|
Q9THY9
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00011
|
40
|
B3FD28
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00012
|
41
|
Q9BAD8
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00013
|
42
|
A0A3G3NI71
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00012
|
43
|
A0A4Y5PPU8
|
~
|
~
|
0
|
0
|
0.00015
|
0.00010
|
0
|
0.00011
|
44
|
A0A3G4R1V6
|
~
|
~
|
0
|
0
|
0
|
0.00010
|
0
|
0.00012
|
45
|
A0A4Y5PQN6
|
~
|
~
|
0
|
0
|
0.00015
|
0
|
0
|
0
|
46
|
A0A0M3TGJ0
|
~
|
~
|
0
|
0
|
0
|
0.00010
|
0
|
0
|
47
|
Q9SBY1
|
Ribulose-bisphosphate carboxylase large subunit (Fragment) (Q9SBY1_9ROSI)
|
rbcL
|
0
|
0
|
0.00016
|
0.00011
|
0
|
0.00012
|
48
|
Q9SC24
|
~
|
~
|
0
|
0
|
0
|
0.00011
|
0
|
0.00012
|
49
|
Q9SC93
|
~
|
~
|
0
|
0
|
0.00016
|
0.00011
|
0
|
0.00012
|
50
|
Q9SBW8
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00012
|
51
|
Q9SC53
|
~
|
~
|
0
|
0
|
0.00016
|
0.00011
|
0
|
0
|
52
|
Q9SBY0
|
~
|
~
|
0
|
0
|
0.00016
|
0
|
0
|
0.000115
|
53
|
Q9SBW6
|
~
|
~
|
0
|
0
|
0.00016
|
0
|
0
|
0
|
54
|
Q9SBX9
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00012
|
55
|
Q9SBW7
|
~
|
~
|
0
|
0
|
0
|
0
|
0
|
0.00012
|
56
|
A0A1S6WN95
|
Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (Fragment) (A0A1S6WN95_9ROSI)
|
N/A
|
0
|
0
|
0
|
0.00054
|
0
|
0
|
57
|
A0A411GWE8
|
~
|
rbcL
|
0
|
0
|
0
|
0.00077
|
0
|
0
|
58
|
A0A4Y5PPR9
|
Photosystem I assembly protein Ycf3 (A0A4Y5PPR9_9ROSI)
|
ycf3
|
0.00595
|
0
|
0
|
0
|
0
|
0
|
59
|
A7LHF2
|
Actin (Fragment) (A7LHF2_9ROSI)
|
N/A
|
0
|
0.00503
|
0
|
0
|
0
|
0
|