See the published version of this article at BMC Veterinary Research.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Methodology article
The VetMAXTM M. tuberculosis Complex PCR Kit detects MTBC DNA in antemortem and postmortem samples from White rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer).
Wynand Johan Goosen
Stellenbosch University Faculty of Medicine and Health Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6614-9084
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background: Bovine tuberculosis and tuberculosis are chronic infectious diseases caused by the Mycobacterium tuberculosis complex members, Mycobacterium bovis and Mycobacterium tuberculosis , respectively. Infection with M. bovis and M. tuberculosis have significant implications for wildlife species management, public health, veterinary disease control, and conservation endeavours.
Results : Here we describe the first use of the VetMAX TM Mycobacterium tuberculosis complex (MTBC) DNA quantitative real-time polymerase chain reaction (qPCR) detection kit for African wildlife samples. DNA was extracted from tissues harvested from 48 African buffaloes and MTBC DNA was detected (test-positive) in all 26 M. bovis culture-confirmed animals with an additional 12 PCR-positive results in culture-negative buffaloes (originating from an exposed population). Of six MTBC-infected African rhinoceros tested, MTBC DNA was detected in antemortem and postmortem samples from five animals. The PCR was also able to detect MTBC DNA in samples from two African elephants confirmed to have M. bovis and M. tuberculosis infections (one each). Culture-confirmed uninfected rhinoceros and elephants’ samples tested negative in the PCR assay.
Conclusions: These results suggest this new detection kit is a sensitive screening test for the detection of MTBC-infected African buffaloes, African elephants and white rhinoceros.
Keywords
African buffaloes, African elephants, Mycobacterium bovis, Mycobacterium tuberculosis, PCR, VetMAXTM MTBC Detection Kit qPCR, white rhinoceros
This is a list of supplementary files associated with this preprint. Click to download.
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 25 May, 2020
Published
On 29 Jun, 2020
No community comments so far
Reviewer #2 agreed
On 25 May, 2020
Review #2 received
Received 25 May, 2020
Reviewer #1 agreed
On 22 May, 2020
Reviewers invited
Invitations sent on 21 May, 2020
Editor assigned
On 13 May, 2020
Submission checks complete
On 12 May, 2020
Editor invited
On 12 May, 2020
No comments provided
Editorial decision: Major revision
On 30 Apr, 2020
Review #2 received
Received 15 Mar, 2020
Reviewer #2 agreed
On 01 Mar, 2020
Review #1 received
Received 27 Feb, 2020
Reviewers invited
Invitations sent on 26 Feb, 2020
Reviewer #1 agreed
On 26 Feb, 2020
Editor assigned
On 16 Feb, 2020
Submission checks complete
On 15 Feb, 2020
Editor invited
On 15 Feb, 2020
First submitted
On 13 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Large Animal Medicine
Learn more about our company.
This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Methodology article
The VetMAXTM M. tuberculosis Complex PCR Kit detects MTBC DNA in antemortem and postmortem samples from White rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer).
Wynand Johan Goosen
Stellenbosch University Faculty of Medicine and Health Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6614-9084
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 25 May, 2020
Published
On 29 Jun, 2020
No community comments so far
Reviewer #2 agreed
On 25 May, 2020
Review #2 received
Received 25 May, 2020
Reviewer #1 agreed
On 22 May, 2020
Reviewers invited
Invitations sent on 21 May, 2020
Editor assigned
On 13 May, 2020
Submission checks complete
On 12 May, 2020
Editor invited
On 12 May, 2020
No comments provided
Editorial decision: Major revision
On 30 Apr, 2020
Review #2 received
Received 15 Mar, 2020
Reviewer #2 agreed
On 01 Mar, 2020
Review #1 received
Received 27 Feb, 2020
Reviewers invited
Invitations sent on 26 Feb, 2020
Reviewer #1 agreed
On 26 Feb, 2020
Editor assigned
On 16 Feb, 2020
Submission checks complete
On 15 Feb, 2020
Editor invited
On 15 Feb, 2020
First submitted
On 13 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Large Animal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background: Bovine tuberculosis and tuberculosis are chronic infectious diseases caused by the Mycobacterium tuberculosis complex members, Mycobacterium bovis and Mycobacterium tuberculosis , respectively. Infection with M. bovis and M. tuberculosis have significant implications for wildlife species management, public health, veterinary disease control, and conservation endeavours.
Results : Here we describe the first use of the VetMAX TM Mycobacterium tuberculosis complex (MTBC) DNA quantitative real-time polymerase chain reaction (qPCR) detection kit for African wildlife samples. DNA was extracted from tissues harvested from 48 African buffaloes and MTBC DNA was detected (test-positive) in all 26 M. bovis culture-confirmed animals with an additional 12 PCR-positive results in culture-negative buffaloes (originating from an exposed population). Of six MTBC-infected African rhinoceros tested, MTBC DNA was detected in antemortem and postmortem samples from five animals. The PCR was also able to detect MTBC DNA in samples from two African elephants confirmed to have M. bovis and M. tuberculosis infections (one each). Culture-confirmed uninfected rhinoceros and elephants’ samples tested negative in the PCR assay.
Conclusions: These results suggest this new detection kit is a sensitive screening test for the detection of MTBC-infected African buffaloes, African elephants and white rhinoceros.