Detection of p53 mutation and serum monitoring alert caused by Marek's disease virus
Background: Marek’s disease as a chicken neoplastic disease, brings huge economic losses to the global poultry industry. The tumor suppressor gene, wild type P53, plays a key role in blocking cell cycle, promoting apoptosis and maintaining stability of genome. However, the p53 could become an oncogene from a tumor inhibitory role, if a mutation happened.
Results: The mutation rate of p53 was 60 percent in experimentally and naturally infected chickens. The mutations included point-mutations and deletions, and mostly located in the DNA-binding domain. The mutated P53 can be expressed in tumors of various tissues in an infected chicken. They accumulated in cytoplasm due to the loss of nuclear localization function. Unlike the researches on human cancer, the concentration of P53 serum of MD infected chicken was significantly lower than control group.
Conclusions: The p53 mutations were relevant with the development of MD. Detecting the concertation of P53 and P53 antibody in serum could be helpful for diagnosis and monitor of MD.
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Posted 11 May, 2020
On 24 Aug, 2020
On 26 May, 2020
Received 21 May, 2020
On 06 May, 2020
Invitations sent on 04 May, 2020
On 26 Apr, 2020
On 25 Apr, 2020
On 25 Apr, 2020
On 24 Mar, 2020
Received 21 Mar, 2020
Received 19 Mar, 2020
Received 18 Mar, 2020
On 08 Mar, 2020
Invitations sent on 03 Mar, 2020
On 03 Mar, 2020
On 03 Mar, 2020
On 17 Feb, 2020
On 16 Feb, 2020
On 16 Feb, 2020
Detection of p53 mutation and serum monitoring alert caused by Marek's disease virus
Posted 11 May, 2020
On 24 Aug, 2020
On 26 May, 2020
Received 21 May, 2020
On 06 May, 2020
Invitations sent on 04 May, 2020
On 26 Apr, 2020
On 25 Apr, 2020
On 25 Apr, 2020
On 24 Mar, 2020
Received 21 Mar, 2020
Received 19 Mar, 2020
Received 18 Mar, 2020
On 08 Mar, 2020
Invitations sent on 03 Mar, 2020
On 03 Mar, 2020
On 03 Mar, 2020
On 17 Feb, 2020
On 16 Feb, 2020
On 16 Feb, 2020
Background: Marek’s disease as a chicken neoplastic disease, brings huge economic losses to the global poultry industry. The tumor suppressor gene, wild type P53, plays a key role in blocking cell cycle, promoting apoptosis and maintaining stability of genome. However, the p53 could become an oncogene from a tumor inhibitory role, if a mutation happened.
Results: The mutation rate of p53 was 60 percent in experimentally and naturally infected chickens. The mutations included point-mutations and deletions, and mostly located in the DNA-binding domain. The mutated P53 can be expressed in tumors of various tissues in an infected chicken. They accumulated in cytoplasm due to the loss of nuclear localization function. Unlike the researches on human cancer, the concentration of P53 serum of MD infected chicken was significantly lower than control group.
Conclusions: The p53 mutations were relevant with the development of MD. Detecting the concertation of P53 and P53 antibody in serum could be helpful for diagnosis and monitor of MD.
Figure 1
Figure 2
Figure 3