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Research article
Detection of p53 mutation and serum monitoring alert caused by Marek's disease virus
Sidang Liu
Shandong Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8400-2764
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background: Marek’s disease as a chicken neoplastic disease, brings huge economic losses to the global poultry industry. The tumor suppressor gene, wild type P53, plays a key role in blocking cell cycle, promoting apoptosis and maintaining stability of genome. However, the p53 could become an oncogene from a tumor inhibitory role, if a mutation happened.
Results: The mutation rate of p53 was 60 percent in experimentally and naturally infected chickens. The mutations included point-mutations and deletions, and mostly located in the DNA-binding domain. The mutated P53 can be expressed in tumors of various tissues in an infected chicken. They accumulated in cytoplasm due to the loss of nuclear localization function. Unlike the researches on human cancer, the concentration of P53 serum of MD infected chicken was significantly lower than control group.
Conclusions: The p53 mutations were relevant with the development of MD. Detecting the concertation of P53 and P53 antibody in serum could be helpful for diagnosis and monitor of MD.
Keywords
Marek’s disease, p53, P53 antibody
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CURRENT STATUS: Published
Version 2
Posted 11 May, 2020
Published
On 24 Aug, 2020
No community comments so far
Editorial decision: Major revision
On 26 May, 2020
Review #1 received
Received 21 May, 2020
Reviewer #1 agreed
On 06 May, 2020
Reviewers invited
Invitations sent on 04 May, 2020
Editor assigned
On 26 Apr, 2020
Submission checks complete
On 25 Apr, 2020
Editor invited
On 25 Apr, 2020
No comments provided
Editorial decision: Major revision
On 24 Mar, 2020
Review #2 received
Received 21 Mar, 2020
Review #3 received
Received 19 Mar, 2020
Review #1 received
Received 18 Mar, 2020
Reviewer #3 agreed
On 08 Mar, 2020
Reviewers invited
Invitations sent on 03 Mar, 2020
Reviewer #1 agreed
On 03 Mar, 2020
Reviewer #2 agreed
On 03 Mar, 2020
Editor assigned
On 17 Feb, 2020
Submission checks complete
On 16 Feb, 2020
Editor invited
On 16 Feb, 2020
Metrics
Comments: 0
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Subject Areas
Small Animal Medicine
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This preprint is under consideration at BMC Veterinary Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Detection of p53 mutation and serum monitoring alert caused by Marek's disease virus
Sidang Liu
Shandong Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8400-2764
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 11 May, 2020
Published
On 24 Aug, 2020
No community comments so far
Editorial decision: Major revision
On 26 May, 2020
Review #1 received
Received 21 May, 2020
Reviewer #1 agreed
On 06 May, 2020
Reviewers invited
Invitations sent on 04 May, 2020
Editor assigned
On 26 Apr, 2020
Submission checks complete
On 25 Apr, 2020
Editor invited
On 25 Apr, 2020
No comments provided
Editorial decision: Major revision
On 24 Mar, 2020
Review #2 received
Received 21 Mar, 2020
Review #3 received
Received 19 Mar, 2020
Review #1 received
Received 18 Mar, 2020
Reviewer #3 agreed
On 08 Mar, 2020
Reviewers invited
Invitations sent on 03 Mar, 2020
Reviewer #1 agreed
On 03 Mar, 2020
Reviewer #2 agreed
On 03 Mar, 2020
Editor assigned
On 17 Feb, 2020
Submission checks complete
On 16 Feb, 2020
Editor invited
On 16 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Small Animal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Veterinary Research.
Background: Marek’s disease as a chicken neoplastic disease, brings huge economic losses to the global poultry industry. The tumor suppressor gene, wild type P53, plays a key role in blocking cell cycle, promoting apoptosis and maintaining stability of genome. However, the p53 could become an oncogene from a tumor inhibitory role, if a mutation happened.
Results: The mutation rate of p53 was 60 percent in experimentally and naturally infected chickens. The mutations included point-mutations and deletions, and mostly located in the DNA-binding domain. The mutated P53 can be expressed in tumors of various tissues in an infected chicken. They accumulated in cytoplasm due to the loss of nuclear localization function. Unlike the researches on human cancer, the concentration of P53 serum of MD infected chicken was significantly lower than control group.
Conclusions: The p53 mutations were relevant with the development of MD. Detecting the concertation of P53 and P53 antibody in serum could be helpful for diagnosis and monitor of MD.
Figure 1
Figure 2
Figure 3