In this research, the comparative analysis of heavy metals has been studied. An experiment was conducted to determine heavy metals bioaccumulation in wheat as wheat is the staple cereal crop consumed as food globally. Abiotic stress disturbs the metabolic pathway of plants, and the bioaccumulation of the metals cause enzymatic degradation, reduction in photosynthesis, increased level of the ROS species. Comparative analysis of the Cd, Pb, Cr, Cu, and Ni was analyzed with the help of Inductively Coupled plasma optical emission spectrometry (ICP-OES). The results show that Ni has a maximum value of BCF in shoots and roots, whereas the Cr has maximum TF values. SOD and POD assays were performed to check the ROS level in the plants. The results show that plants increase their anti-oxidant enzymes for the scavenging of ROS produced against the bioaccumulation of heavy metals.