Investigation of the Antimicrobial Activity and Hematological Pattern of Nano-Chitosan and its Developed Nano-Copper Composite
Novel synthesized Chitosan-Copper oxide nanocomposite (Cs-CuO) was prepared using pomegranate peels extract as green precipitating agents to improve the biological activity of Cs-NP's which was synthesized through ionic gelation method. The characterization of biogenic nanoparticles Cs-NP's and Cs-CuO-NP's were investigated structurally, morphologically to determine the full descriptive features of those nanoparticles. Antimicrobial activity was tested for both Cs-NP's and Cs-CuO-NP's via Minimum inhibition concentration and zone analysis against fungus, gram positive and gram negative. The results of the antimicrobial test showed high sensitivity of Cs-CuO-NP's to all microorganisms that are tested in concentration less than 20 mg/ml while the sensitivity of Cs-NP's against all microorganisms under test started from a concentration of 20 mg/ml to 40 mg/ml except for the C.albicans species. Hematological activity was also tested in via measuring the RBCs, platelets count and clotting time against healthy, diabetic and hypercholesterolic blood samples. Measurement showed a decrease in RBCs and platelets count by adding Cs-NP’s or Cs-CuO-NP's to the three blood samples. Cs-NP’s success to decrease the clotting time for healthy and diabetic blood acting as a procoagulant agent, while adding biogenic CuO-NP’s to Cs-NP’s increased clotting time considering as an anticoagulant agent for hyperchloesterolic blood samples.
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Posted 21 Jan, 2021
Received 24 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
Invitations sent on 09 Feb, 2021
On 05 Feb, 2021
On 20 Jan, 2021
On 19 Jan, 2021
On 17 Jan, 2021
Investigation of the Antimicrobial Activity and Hematological Pattern of Nano-Chitosan and its Developed Nano-Copper Composite
Posted 21 Jan, 2021
Received 24 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
On 09 Feb, 2021
Invitations sent on 09 Feb, 2021
On 05 Feb, 2021
On 20 Jan, 2021
On 19 Jan, 2021
On 17 Jan, 2021
Novel synthesized Chitosan-Copper oxide nanocomposite (Cs-CuO) was prepared using pomegranate peels extract as green precipitating agents to improve the biological activity of Cs-NP's which was synthesized through ionic gelation method. The characterization of biogenic nanoparticles Cs-NP's and Cs-CuO-NP's were investigated structurally, morphologically to determine the full descriptive features of those nanoparticles. Antimicrobial activity was tested for both Cs-NP's and Cs-CuO-NP's via Minimum inhibition concentration and zone analysis against fungus, gram positive and gram negative. The results of the antimicrobial test showed high sensitivity of Cs-CuO-NP's to all microorganisms that are tested in concentration less than 20 mg/ml while the sensitivity of Cs-NP's against all microorganisms under test started from a concentration of 20 mg/ml to 40 mg/ml except for the C.albicans species. Hematological activity was also tested in via measuring the RBCs, platelets count and clotting time against healthy, diabetic and hypercholesterolic blood samples. Measurement showed a decrease in RBCs and platelets count by adding Cs-NP’s or Cs-CuO-NP's to the three blood samples. Cs-NP’s success to decrease the clotting time for healthy and diabetic blood acting as a procoagulant agent, while adding biogenic CuO-NP’s to Cs-NP’s increased clotting time considering as an anticoagulant agent for hyperchloesterolic blood samples.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5