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Complete genome of the barotolerant Listeria monocytogenes RO15 strain and comparison with other strains isolated from food and food processing environments
Ilhan Cem Duru
Institute of Biotechnology, University of Helsinki
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3409-5215
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background: High pressure processing (HPP; i.e. 100 - 600 MPa pressure depending on product) is a non-thermal preservation technique adopted by the food industry to decrease significantly foodborne pathogens, including Listeria monocytogenes, from food. However, susceptibility towards pressure differs among diverse strains of L. monocytogenes and it is unclear if this is related to their genomic content. Here, we tested the barotolerance of 10 different L. monocytogenes strains, from food and food processing environments and widely used reference type strains, to pressure treatments with 400 and 600 MPa. Genome sequencing and genome comparison of the tested L. monocytogenes strains were performed to investigate the relation between genomic profile and pressure tolerance.
Results: None of the tested strains were tolerant to 600 MPa. A reduction of more than 5 log10 was observed for all strains after 1 minute 600 MPa pressure treatment. L. monocytogenes strain RO15 showed no significant reduction in viable cell counts after 400 MPa for 1 minute and was therefore defined as barotolerant. Genome analysis of so far unsequenced L. monocytogenes strain RO15, 2HF33, MB5, AB199, AB120, C7, and RO4 allowed us to compare the gene content of all strains tested. This revealed that the three most pressure tolerant strains had more than one CRISPR system with self-targeting spacers. Furthermore, several anti-CRISPR genes were detected in these strains. Pan-genome analysis showed that 10 prophage genes were significantly associated with the three most barotolerant strains.
Conclusions: L. monocytogenes strain RO15 was the most pressure tolerant among the selected strains. Genome comparison suggests that there might be a relationship between prophages and pressure tolerance in L. monocytogenes.
Keywords
high-pressure processing (HPP), foodborne pathogen, CRISPR, PacBio, comparative genomics, pan-genome.
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Reviewer #2 agreed
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Learn more about In Review
Research article
Complete genome of the barotolerant Listeria monocytogenes RO15 strain and comparison with other strains isolated from food and food processing environments
Ilhan Cem Duru
Institute of Biotechnology, University of Helsinki
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3409-5215
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 13 May, 2020
Published
On 02 Jul, 2020
No community comments so far
Review #1 received
Received 19 May, 2020
Review #2 received
Received 18 May, 2020
Review #3 received
Received 18 May, 2020
Reviewer #1 agreed
On 28 Apr, 2020
Reviewer #2 agreed
On 28 Apr, 2020
Reviewer #3 agreed
On 28 Apr, 2020
Editor assigned
On 27 Apr, 2020
Reviewers invited
Invitations sent on 27 Apr, 2020
Editor invited
On 26 Apr, 2020
Submission checks complete
On 24 Feb, 2020
No comments provided
Editorial decision: Major revision
On 25 Mar, 2020
Review #4 received
Received 20 Mar, 2020
Review #3 received
Received 19 Mar, 2020
Review #2 received
Received 19 Mar, 2020
Review #1 received
Received 18 Mar, 2020
Reviewer #5 agreed
On 07 Mar, 2020
Reviewer #3 agreed
On 06 Mar, 2020
Reviewer #4 agreed
On 06 Mar, 2020
Reviewer #2 agreed
On 04 Mar, 2020
Reviewer #1 agreed
On 03 Mar, 2020
Reviewers invited
Invitations sent on 02 Mar, 2020
Editor invited
On 24 Feb, 2020
Editor assigned
On 24 Feb, 2020
Submission checks complete
On 23 Feb, 2020
First submitted
On 21 Feb, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomics.
Background: High pressure processing (HPP; i.e. 100 - 600 MPa pressure depending on product) is a non-thermal preservation technique adopted by the food industry to decrease significantly foodborne pathogens, including Listeria monocytogenes, from food. However, susceptibility towards pressure differs among diverse strains of L. monocytogenes and it is unclear if this is related to their genomic content. Here, we tested the barotolerance of 10 different L. monocytogenes strains, from food and food processing environments and widely used reference type strains, to pressure treatments with 400 and 600 MPa. Genome sequencing and genome comparison of the tested L. monocytogenes strains were performed to investigate the relation between genomic profile and pressure tolerance.
Results: None of the tested strains were tolerant to 600 MPa. A reduction of more than 5 log10 was observed for all strains after 1 minute 600 MPa pressure treatment. L. monocytogenes strain RO15 showed no significant reduction in viable cell counts after 400 MPa for 1 minute and was therefore defined as barotolerant. Genome analysis of so far unsequenced L. monocytogenes strain RO15, 2HF33, MB5, AB199, AB120, C7, and RO4 allowed us to compare the gene content of all strains tested. This revealed that the three most pressure tolerant strains had more than one CRISPR system with self-targeting spacers. Furthermore, several anti-CRISPR genes were detected in these strains. Pan-genome analysis showed that 10 prophage genes were significantly associated with the three most barotolerant strains.
Conclusions: L. monocytogenes strain RO15 was the most pressure tolerant among the selected strains. Genome comparison suggests that there might be a relationship between prophages and pressure tolerance in L. monocytogenes.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5