Human bone marrow mesenchymal stem cells-derived exosomes stimulate cutaneous wound healing mediates through TGF-β/Smad signaling pathway
Background: Cutaneous wound healing represents a morphogenetic response to injury, and is designed to restore anatomic and physiological function. Human bone marrow mesenchymal stem cells-derived exosomes (hBM-MSCs-Ex) is a promising source for cell-free therapy and skin regeneration.
Methods: In this study, we investigated the cell regeneration effects and its underlying mechanism of hBM-MSCs-Ex on cutaneous wound healing in rats. In vitro studies , w e evaluated the role of hBM-MSCs-Ex in the two type s of skin cell s : human keratinocytes (HaCaT) and human dermal fibroblasts (HDFs) for the proliferation . For in vivo studies , we used a full-thickness skin wound model to evaluate the effects of hBM-MSCs-Ex on cutaneous wound healing in vivo .
Results: The results demonstrated that hBM-MSCs-Ex promote both two type s of skin cell s growth effectively and accelerate the cutaneous wound healing. Interestingly , we found that hBM-MSCs-Ex significantly down-regulated TGF-β1, Smad2, Smad3, and Smad4 expression, while up-regulated TGF-β3 and Smad7 expression in the TGF-β/Smad signaling pathway .
Conclusions: Our findings indicated that hBM-MSCs-Ex effectively promote the cutaneous wound healing through inhibiting the TGF-β/Smad signal pathway . The current result s providing a new sight for the therapeutic strategy for the treatment of cutaneous wounds.
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Posted 19 May, 2020
On 07 May, 2020
On 06 May, 2020
On 06 May, 2020
On 05 May, 2020
Received 24 Apr, 2020
On 17 Apr, 2020
On 14 Apr, 2020
Invitations sent on 14 Apr, 2020
On 13 Apr, 2020
On 13 Apr, 2020
On 16 Mar, 2020
Received 11 Mar, 2020
Received 11 Mar, 2020
On 01 Mar, 2020
On 01 Mar, 2020
On 27 Feb, 2020
Received 27 Feb, 2020
On 27 Feb, 2020
Invitations sent on 27 Feb, 2020
On 26 Feb, 2020
On 25 Feb, 2020
On 22 Feb, 2020
Human bone marrow mesenchymal stem cells-derived exosomes stimulate cutaneous wound healing mediates through TGF-β/Smad signaling pathway
Posted 19 May, 2020
On 07 May, 2020
On 06 May, 2020
On 06 May, 2020
On 05 May, 2020
Received 24 Apr, 2020
On 17 Apr, 2020
On 14 Apr, 2020
Invitations sent on 14 Apr, 2020
On 13 Apr, 2020
On 13 Apr, 2020
On 16 Mar, 2020
Received 11 Mar, 2020
Received 11 Mar, 2020
On 01 Mar, 2020
On 01 Mar, 2020
On 27 Feb, 2020
Received 27 Feb, 2020
On 27 Feb, 2020
Invitations sent on 27 Feb, 2020
On 26 Feb, 2020
On 25 Feb, 2020
On 22 Feb, 2020
Background: Cutaneous wound healing represents a morphogenetic response to injury, and is designed to restore anatomic and physiological function. Human bone marrow mesenchymal stem cells-derived exosomes (hBM-MSCs-Ex) is a promising source for cell-free therapy and skin regeneration.
Methods: In this study, we investigated the cell regeneration effects and its underlying mechanism of hBM-MSCs-Ex on cutaneous wound healing in rats. In vitro studies , w e evaluated the role of hBM-MSCs-Ex in the two type s of skin cell s : human keratinocytes (HaCaT) and human dermal fibroblasts (HDFs) for the proliferation . For in vivo studies , we used a full-thickness skin wound model to evaluate the effects of hBM-MSCs-Ex on cutaneous wound healing in vivo .
Results: The results demonstrated that hBM-MSCs-Ex promote both two type s of skin cell s growth effectively and accelerate the cutaneous wound healing. Interestingly , we found that hBM-MSCs-Ex significantly down-regulated TGF-β1, Smad2, Smad3, and Smad4 expression, while up-regulated TGF-β3 and Smad7 expression in the TGF-β/Smad signaling pathway .
Conclusions: Our findings indicated that hBM-MSCs-Ex effectively promote the cutaneous wound healing through inhibiting the TGF-β/Smad signal pathway . The current result s providing a new sight for the therapeutic strategy for the treatment of cutaneous wounds.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5