1. Apfelbaum, J. L., Chen, C., Mehta, S. S. & Gan, T. J. Postoperative pain experience: Results from a national survey suggest postoperative pain continues to be undermanaged. Anesth. Analg. 97, 534–540 (2003).
2. Lorentzen, V., Hermansen, I. L. & Botti, M. A prospective analysis of pain experience, beliefs and attitudes, and pain management of a cohort of Danish surgical patients. Eur. J. Pain 16, 278–288 (2012).
3. Kharasch, E. D. & Michael Brunt, L. Perioperative opioids and public health. Anesthesiology 124, 960–965 (2016).
4. Popoff, M. R. & Bouvet, P. Genetic characteristics of toxigenic Clostridia and toxin gene evolution. Toxicon 75, 63–89 (2013).
5. Pirazzini, M., Rossetto, O., Eleopra, R. & Montecucco, C. Botulinum neurotoxins: Biology, pharmacology, and toxicology. Pharmacological Reviews vol. 69 (2017).
6. Pavone, F. & Luvisetto, S. Botulinum neurotoxin for pain management: Insights from animal models. Toxins (Basel). 2, 2890–2913 (2010).
7. Matak, I. & Lacković, Z. Botulinum toxin A, brain and pain. Prog. Neurobiol. 119–120, 39–59 (2014).
8. Castel, D., Willentz, E., Doron, O., Brenner, O. & Meilin, S. Characterization of a porcine model of post-operative pain. Eur. J. Pain (United Kingdom) 18, 496–505 (2014).
9. Swindle, M. M., Makin, A., Herron, A. J., Clubb, F. J. & Frazier, K. S. Swine as Models in Biomedical Research and Toxicology Testing. Vet. Pathol. 49, 344–356 (2012).
10. Summerfield, A., Meurens, F. & Ricklin, M. E. The immunology of the porcine skin and its value as a model for human skin. Mol. Immunol. 66, 14–21 (2015).
11. Brennan, T. J. Pathophysiology of postoperative pain. Pain 152, 33–40 (2011).
12. Cooper, S. A. et al. Research design considerations for single-dose analgesic clinical trials in acute pain: IMMPACT recommendations. Pain 157, 288–301 (2016).
13. Srikandarajah, S. & Gilron, I. Systematic review of movement-evoked pain versus pain at rest in postsurgical clinical trials and meta-analyses: A fundamental distinction requiring standardized measurement. Pain 152, 1734–1739 (2011).
14. Brennan, T. J., Vandermeulen, E. P. & Gebhart, G. F. Characterization of a rat model of incisional pain. Pain 64, 493–502 (1996).
15. Ghoneim, M. M. & O’Hara, M. W. Depression and postoperative complications: An overview Visceral and general surgery. BMC Surg. 16, 1–10 (2016).
16. Kouya, F., Iqbal, Z., Charen, D., Shah, M. & Banik, R. K. Evaluation of anxiety-like behaviour in a rat model of acute postoperative pain. Eur. J. Anaesthesiol. 32, 242–247 (2015).
17. Su, C. et al. Persistent pain alters AMPA receptor subunit levels in the nucleus accumbens. Mol. Brain 8, 1–17 (2015).
18. Filipović, B., Bach-Rojecky, L. & Lacković, Z. Lasting reduction of postsurgical hyperalgesia after single injection of botulinum toxin type A in rat. Fundam. Clin. Pharmacol. 24, 43–45 (2010).
19. Li, X. et al. Botulinum toxin type A and gabapentin attenuate postoperative pain and NK1 receptor internalization in rats. Neurochem. Int. 116, 52–62 (2018).
20. Vilhegas, S. et al. Botulinum toxin type A as an adjunct in postoperative pain management in dogs undergoing radical mastectomy. Vet. Rec. 177, 391 (2015).
21. Layeeque, R. et al. Botulinum toxin infiltration for pain control after mastectomy and expander reconstruction. Ann. Surg. 240, 608–614 (2004).
22. Hamdy, R. C. et al. Botulinum toxin type a injection in alleviating postoperative pain and improving quality of life in lower extremity limb lengthening and deformity correction: A pilot study. J. Pediatr. Orthop. 29, 427–434 (2009).
23. Magid, M. et al. Treatment of major depressive disorder using botulinum toxin a: A 24-week randomized, double-blind, placebo-controlled study. J. Clin. Psychiatry 75, 837–844 (2014).
24. Magid, M. et al. Treating Depression with Botulinum Toxin: A Pooled Analysis of Randomized Controlled Trials. Pharmacopsychiatry 48, 205–210 (2015).
25. Makunts, T., Wollmer, M. A. & Abagyan, R. Postmarketing safety surveillance data reveals antidepressant effects of botulinum toxin across various indications and injection sites. Sci. Rep. 10, 1–9 (2020).
26. Finzi, E. & Rosenthal, N. E. Emotional proprioception: Treatment of depression with afferent facial feedback. J. Psychiatr. Res. 80, 93–96 (2016).
27. Antonucci, F., Rossi, C., Gianfranceschi, L., Rossetto, O. & Caleo, M. Long-distance retrograde effects of botulinum neurotoxin A. J. Neurosci. 28, 3689–3696 (2008).
28. Bach-Rojecky, L. & Lacković, Z. Central origin of the antinociceptive action of botulinum toxin type A. Pharmacol. Biochem. Behav. 94, 234–238 (2009).
29. Matak, I., Bach-Rojecky, L., Filipović, B. & Lacković, Z. Behavioral and immunohistochemical evidence for central antinociceptive activity of botulinum toxin A. Neuroscience 186, 201–207 (2011).
30. Matak, I., Riederer, P. & Lacković, Z. Botulinum toxin’s axonal transport from periphery to the spinal cord. Neurochem. Int. 61, 236–239 (2012).
31. Caleo, M. et al. Transynaptic action of botulinum neurotoxin type a at central cholinergic boutons. J. Neurosci. 38, 10329–10337 (2018).
32. Hawlitschka, A. & Wree, A. Experimental intrastriatal applications of botulinum neurotoxin-a: A review. Int. J. Mol. Sci. 19, 1–20 (2018).
33. Bomba-Warczak, E. et al. Interneuronal Transfer and Distal Action of Tetanus Toxin and Botulinum Neurotoxins A and D in Central Neurons. Cell Rep. 16, 1974–1987 (2016).
34. Matak, I., Riederer, P. & Lacković, Z. Botulinum toxin’s axonal transport from periphery to the spinal cord. Neurochem. Int. 61, 236–239 (2012).
35. Obata, H., Eisenach, J. C., Hussain, H., Bynum, T. & Vincler, M. Spinal Glial Activation Contributes to Postoperative Mechanical Hypersensitivity in the Rat. J. Pain 7, 816–822 (2006).
36. Romero-Sandoval, A. & Eisenach, J. C. Spinal cannabinoid receptor type 2 activation reduces hypersensitivity and spinal cord glial activation after paw incision. Anesthesiology 106, 787–794 (2007).
37. Romero-Sandoval, A., Chai, N., Nutile-McMenemy, N. & DeLeo, J. A. A comparison of spinal Iba1 and GFAP expression in rodent models of acute and chronic pain. Brain Res. 1219, 116–126 (2008).
38. Sahbaie, P. et al. Role of substance P signaling in enhanced nociceptive sensitization and local cytokine production after incision. Pain 145, 341–349 (2009).
39. Saur, L. et al. Physical exercise increases GFAP expression and induces morphological changes in hippocampal astrocytes. Brain Struct. Funct. 219, 293–302 (2014).
40. Imai, Y., Ibata, I., Ito, D., Ohsawa, K. & Kohsaka, S. A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage. Biochem. Biophys. Res. Commun. 224, 855–862 (1996).
41. Hovens, I., Nyakas, C. & Schoemaker, R. A novel method for evaluating microglial activation using ionized calcium-binding adaptor protein-1 staining: cell body to cell size ratio. Neuroimmunol. Neuroinflammation 1, 82 (2014).
42. Favre-Guilmard, C., Auguet, M. & Chabrier, P. E. Different antinociceptive effects of botulinum toxin type A in inflammatory and peripheral polyneuropathic rat models. Eur. J. Pharmacol. 617, 48–53 (2009).
43. Favre-Guilmard, C., Chabrier, P.-E. & Kalinichev, M. Bilateral analgesic effects of abobotulinumtoxinA (Dysport®) following unilateral administration in the rat. Eur. J. Pain (United Kingdom) 21, (2017).
44. Bach-Rojecky, L., Šalković-Petrišić, M. & Lacković, Z. Botulinum toxin type A reduces pain supersensitivity in experimental diabetic neuropathy: Bilateral effect after unilateral injection. Eur. J. Pharmacol. 633, 10–14 (2010).
45. Ishida, K., Kawamata, T., Tanaka, S., Shindo, T. & Kawamata, M. Calcitonin Gene – related Peptide Is Involved in. 1068–1079 (2014).
46. Chen, Y. W. et al. High-frequency transcutaneous electrical nerve stimulation attenuates postsurgical pain and inhibits excess substance P in rat dorsal root ganglion. Reg. Anesth. Pain Med. 39, 322–328 (2014).
47. Zimmermann, M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 16, 109–110 (1983).
48. Pérez Fraga, P., Gerencsér, L. & Andics, A. Human proximity seeking in family pigs and dogs. Sci. Rep. 10, 1–11 (2020).
49. Castel, D., Sabbag, I., Nasaev, E., Peng, S. & Meilin, S. Open field and a behavior score in PNT model for neuropathic pain in pigs. J. Pain Res. 11, 2279–2293 (2018).
50. Perier, Cindy; Martin, Vincent; Cornet, Sylvie; Favre-Guilmard, Christine; Rocher, Marie-Noelle; Bindler, Julien; Wagner Stephanie; Andriambeloson, Emile; Vignaud, Alban; Beard Matthew; Lezmi, Stephane; Kalinichev, M. Recombinant botulinum neurotoxin serotype A1 in vivo characterization. Pharmacol. Res. Perspect. (2021).