Objective: To study the network mechanism of qufeng Tongqiao Prescription in the treatment of cerebral ischemia-reperfusion related genes through network screening and molecular docking verification.
Methods: Venny diagram, GO and KEGG analysis, PPI protein interaction, Hub gene screening, molecular verification and animal behavior experiments were performed to conduct inflammation through network pharmacology.
Results: There were 53 intersecting genes of Qufengtongqiao Recipe and cerebral ischemia reperfusion. GO analysis showed that the main BP was response to lipopolysaccharide, and the main CC process was membrane raft. Cytokine receptor binding is the most important MF process.Age-rage signaling pathway in diabetic complications is the most important signaling pathway in KEGG pathway.Through molecular docking, it was found that Astragalus membranaceus was docked with MAPK14, IL4, FOS, IL6 and JUN, pueraria membranaceus was directly docked with JUN and IL4, Acorus acorus was linked to JUN and MAPK14, Ganoderma ganoderma and human were involved in JUN docking, and Ligusticum chuanqi and pueraria could not be docked with MAPK14 respectively.
Conclusion: Qufeng Tongqiao prescription has therapeutic effect on cerebral ischemia reperfusion.