Background: Liver fibrosis is characterized by irreversible damage to liver parenchyma. The mechanism of fibrosis is through activation of nuclear factor-kappa (NF-kβ), collagen, activation of necrosis, caspase-3, apoptosis and inhibition of proliferation. The HepG2 cell line is a human liver cancer cell, which is developed and has a human hormonal response. Hepatocyte stem cells have anti-fibrotic, anti-inflammatory, immunomodulating properties, inhibit apoptosis and necrosis, as well as increase proliferation.
Methods: This study is an in vitro pre and posttest experimental study. Hepatocyte stem cells were taken from the umbilical cord of newborns. Cell lines are made into 4 groups. Examination was carried out on NF-kβ, Caspase-3, Apoptosis, Necrosis, Proliferation, and cytopathologically.
Results: Cytopathological test of cell line hepG2 showed fibroblast growth. Mesenchymal stem cells decreased NF-kβ activity. Mesenchymal stem cells did not decrease Caspase3 activity. Mesenchymal Stem Cells significantly decreased Apoptotic activity. Mesenchymal Stem Cells decreased Necrosis activity, while sylimarin was not significant. Mesenchymal Stem Cells increased the Proliferation significantly (p<0.001), while sylimarin was not significant.
Conclusion: Mesenchymal stem cells significantly inhibited NF-kβ, necrosis, apoptosis, and proliferation activation, whereas caspase-3 inhibition was not significant.