Background: This study was conducted to examine the differences in the osteogenic ability of bone marrow mesenchymal stem cells (BMSCs) in patients with hemifacial microsomia on the healthy side versus on the affected side of the mandible.
Methods: Patients with hemifacial microsomia admitted to Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine received bone marrow blood from the healthy and affected sides of the mandible, and BMSCs were cultured to evaluate their osteogenic induction and differentiation activities. Alizarin red (AR) staining was performed to observe the mineralization nodule formation ability of BMSCs. Alkaline phosphatase (ALP) staining was used to measure alkaline phosphatase activity, and the gene expression of the osteogenic-related indicators osteocalcin, osteopontin, Runx-related transcription factor 2, and ALP was detected by real-time fluorescent quantitative PCR. Differences in osteogenic capacity were also explored.
Results: Osteogenesis related staining and Quantitative real-time PCR were performed，the results showed:in AR staining, osteogenesis on the healthy side was significantly better than that on the affected side (P＜0.001) ;in ALP staing, ALP activity of the affected side was significantly lower than that of the healthy side(P＜0.001);in the Quantitative real-time PCR,the osteogenesis-related genes ALP, OCN, OPN, and RUNX2 showed high expression on the healthy side and low expression on the affected side(P＜0.05).
Conclusions: The osteogenesis ability of BMSCs differed on the healthy and affected mandibular bone in patients with hemifacial microsomia; the osteogenesis ability on the affected side was significantly lower than that on the healthy side. Keywords: hemifacial microsomia; bone marrow-derived mesenchymal stem cells; osteogenesis