The ethyl acetate and diethyl ether extracts of the peel of the fruit of Solanum incanum. (S. incanum) was analyzed using gas chromatography-mass spectrometry (GC-MS). 105 compounds were identified in the diethyl ether extract and 75 compounds were identified in ethyl acetate extract. Among them, the mass spectral data of 5 compounds were analyzed, discussed and compared with NIST database. The antibacterial screening was also conducted for both the diethyl ether and ethyl acetate fraction of the fruit peel S.incanum using four pathogens, two Gram positive bacteria Staphylococcus aureus ( S. aureus ) and Streptococcus pyogenes ( S. pyogenes ) ant two Gram negative bacteria Escherichia coli ( E. coli ), Klebsiella pneumoniae ( K. pneumonia ), at four different concentrations (250, 500, 750 and 1000 μg/mL). The diethyl ether and ethyl acetate extracts of the peel of S. incanum exhibited activity against E. coli and K. pneumonia at 1000 μg/mL concentration