Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality
Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet.
Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC).
Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 hours, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 hours, and increased after being co-cultured with Fer-1(P<0.05).
Conclusion Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.
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Posted 20 May, 2020
Received 15 May, 2020
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On 28 Apr, 2020
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Received 24 Apr, 2020
On 23 Apr, 2020
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Received 23 Mar, 2020
Received 23 Mar, 2020
Received 19 Mar, 2020
On 11 Mar, 2020
On 10 Mar, 2020
Invitations sent on 08 Mar, 2020
On 08 Mar, 2020
On 08 Mar, 2020
On 04 Mar, 2020
On 04 Mar, 2020
On 03 Mar, 2020
On 03 Mar, 2020
Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affects semen quality
Posted 20 May, 2020
Received 15 May, 2020
On 11 May, 2020
On 11 May, 2020
On 10 May, 2020
Invitations sent on 10 May, 2020
On 09 May, 2020
On 09 May, 2020
On 28 Apr, 2020
Received 27 Apr, 2020
Received 24 Apr, 2020
On 23 Apr, 2020
Invitations sent on 20 Apr, 2020
On 20 Apr, 2020
On 20 Apr, 2020
On 16 Apr, 2020
On 15 Apr, 2020
On 15 Apr, 2020
On 26 Mar, 2020
Received 23 Mar, 2020
Received 23 Mar, 2020
Received 19 Mar, 2020
On 11 Mar, 2020
On 10 Mar, 2020
Invitations sent on 08 Mar, 2020
On 08 Mar, 2020
On 08 Mar, 2020
On 04 Mar, 2020
On 04 Mar, 2020
On 03 Mar, 2020
On 03 Mar, 2020
Purpose The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet.
Methods In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC).
Results Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P<0.05). However, the level of lipid ROS and iron were significantly increased (P<0.05). Besides, GSH level was reduced following treatment with CSC for 24 hours, while lipid ROS and iron levels were increased (P<0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P<0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 hours, and increased after being co-cultured with Fer-1(P<0.05).
Conclusion Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.
Figure 1
Figure 2
Figure 3