Naupactus cervinus is a parthenogenetic weevil native to South America that is currently distributed worldwide. This flightless species is polyphagous and capable of modifying gene expression regimes for responding to stressful situations. Naupactus cervinus was first reported in the Continental US in 1874 and has rapidly colonized most of the world since. Previous studies suggested that an invader genotype successfully established even in areas of unsuitable environmental conditions. In the present work we analyze mitochondrial and nuclear sequences from 59 individuals collected in 13 localities across three states in the Southern US in order to describe the genetic diversity in this area of introduction not previously studied. Our results suggest that 97% of the samples carry the most prevalent invader genotype already reported, while a few show a novel mitochondrial derivative. This would support the hypothesis of a general purpose genotype capable of coping with adverse conditions and enlarging its geographical range. Given the historical introduction records and the prevalence of the invader genotype, it is possible that the continental US may act as a secondary source of introductions to other areas. Parthenogenesis and its associated lack of recombination could be useful in maintaining the linkage of genetic variants that facilitate colonization of distant, inadequate areas. We propose that scarce genetic variation in places of introduction may in fact be an asset that allows N . cervinus to thrive across a range of environmental conditions.