In this study, which was performed to compare the mechanical properties of rabbit and human decellular skins for alternative use in wound tissue engineering, the results showed that human and rabbit dermal scaffolds after decellularization process, despite showing a decrease in elasticity, the difference They have no significance in terms of elasticity. Also, in the study of the degree of elasticity between rabbit decellular dermis and human decellular dermis, it was observed that despite the larger yang modulus in rabbit skin scaffold, but this difference is not significant. When comparing the data with what is in the background, it was found that there is a big difference between the researchers' reports in terms of rabbit skin yang modulus and human skin yang modulus. Of course, this is to be expected when it comes to tissue and biological conditions, as the determinants of a person's genetics and age, as well as the nature of the skin and the test conditions. For example, in the present study, the Young's modulus for human control was 0.052 ± 0.039, but in Jansen & Rottier's research, which also examined the amount of traction in the laboratory, the Young's modulus was reported to be in the range of 1.07 to 2.157 MPa (12). However, this interval was consistent with the results of the present study in many studies. For example, in the study of Park et al. (13), the Young modulus was reported to be 0.006 to 0.0289 MPa; in the study by Tupin et al (14). A report by Koene et al.(15), Which reviewed reports of Young's modulus range of human skin, ranged from 0.02 to 0.3 MPa (21). Due to this range, the results obtained from the present study are in this usual range. The difference between the values obtained from this study and Jansen & Rottier's study can be related to the experimental conditions and tools used. Jansen & Rottier's research was conducted in 1958 with non-standardized samples. Also, in the study of these researchers, the amount of time elapsed after sampling was not mentioned; The time interval could have a large impact on the test results, and even the values reported were not within the range reported by Koene and many other studies.
The values obtained for rabbit skin were 0.047 ± 0.023, which is in the range of elasticity in human skin, and the results of the analysis showed that these values, despite the difference, are not significantly different. The aim of this study was to investigate the extent of rabbit skin elasticity as an alternative in tissue engineering applications; Since compatibility for transplantation is also important in tissue engineering, rabbit and human decellular scaffolds were also compared. The values obtained showed that after decellularization with the same method for both rabbit and human tissues, although the amount of elasticity in both species has decreased, but this range is still in the range that can provide the required elasticity in providing engineered tissue alternatives.
According to research reports, various factors can affect the amount of skin elasticity, including the location of the sample (16,17). In this study, to prevent the effect of this factor, all human samples were collected from almost one position. Also, the underlying tissues, including subcutaneous fat, are not seen in rabbits, but in humans, this layer is relatively thick. It was removed to examine only the dermis in both species. According to the literature, subcutaneous fat can affect the rate of skin contraction (12).
The time after death and the duration of separation of samples from the primary tissue can also affect the amount of skin elasticity. This study tried to test the samples quickly after sampling, and due to the sensitivity of this tissue to drought, Moisture of the samples was provided at the time of testing. Observance of all these cases in previous research has been done in only a few studies or at least reported.
This research also had some limitations. One of the limitations of this study was the type of sampling that was done in an accessible way. This in itself will reduce the generalizability of the results. Finally, for future research, it is suggested to study the biocompatibility effects of this animal scaffold on a cellular basis in order to provide the necessary conditions to replace this tissue product in tissue-engineered scaffolding.