Glucose both is the favorite carbon and energy source and acts as a hormone that plays a regulated role in many biological processes. Calorie restriction extended lifespan in many organisms, including Schizosaccharomyces pombe, while uptake of high glucose led to undesired results, such as diabetes and aging.
Methods and Results
In this study, sequence analysis of Schizosaccharomyces pombe ird5 and ird11 mutants was performed using next-generation sequencing techniques and a total of 20 different mutations were detected. ird11 is resistant to oxidative stress without calorie restriction, whereas ird5 displays an adaptive response against oxidative stress. Candidate 9 mutations, which are thought to be responsible for ird5 and ird11 mutant phenotypes, were investigated via forward and reverse mutations by using various cloning techniques.
The results of this study contribute to the basic sciences by showing the relationship between glucose sensing/signaling and oxidative stress response components.