See the published version of this preprint at Military Medical Research.
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Research
Sheng Yi
Nantong University
Corresponding Author
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Background: Cytokines are essential cellular modulators of a variety of physiological and pathological activities, including peripheral nerve repair and regeneration. However, the molecular changes of these cellular mediators after peripheral nerve injury are not well clarified. The study is aimed to discover critical cytokines for the regenerative process of injured peripheral nerves.
Methods: The sequencing data of the injured nerve stumps and the dorsal root ganglia (DRGs) of Sprague-Dawley (SD) rats subjected to sciatic nerve (SN) crush injury were analyzed to determine expression patterns of genes coding for cytokines. PCR experiments were used to validate the accuracy of sequencing data.
Results: A total of 46, 52, and 54 upstream cytokines were differentially expressed in SNs at 1 day, 4 days, and 7 days after nerve injury. And a total of 25, 28, and 34 upstream cytokines were differentially expressed in DRGs at these time points. The expression patterns of some essential upstream cytokines were displayed in a heatmap and validated by PCR experiment. Bioinformatic analysis of these differentially expressed upstream cytokines after nerve injury demonstrated that inflammatory and immune responses were significantly involved.
Conclusions: In summary, these findings provided an overview of the dynamic changes of cytokines in SNs and DRGs at different time points after rat nerve crush injury, elucidated the biological processes of differentially expressed cytokines, especially the important roles in inflammatory and immune responses after peripheral nerve injury, and thus might contribute to identification of potential treatments for peripheral nerve repair and regeneration.
Keywords
peripheral nerve injury; rat sciatic nerve crush injury; sciatic nerve stumps; dorsal root ganglia; upstream cytokines.
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CURRENT STATUS: Published
View the published article at Military Medical Research.
Version 2
Posted 20 May, 2020
No community comments so far
Editorial decision: Accept
On 04 Nov, 2020
Review #1 received
Received 29 May, 2020
Review #3 received
Received 19 May, 2020
Reviewer #2 agreed
On 18 May, 2020
Reviewer #3 agreed
On 18 May, 2020
Review #2 received
Received 18 May, 2020
Reviewer #1 agreed
On 16 May, 2020
Reviewers invited
Invitations sent on 16 May, 2020
Editor assigned
On 10 May, 2020
Submission checks complete
On 09 May, 2020
Editor invited
On 09 May, 2020
No comments provided
Editorial decision: Minor revision
On 24 Apr, 2020
Review #3 received
Received 22 Apr, 2020
Reviewer #4 agreed
On 17 Apr, 2020
Review #1 received
Received 27 Mar, 2020
Review #2 received
Received 27 Mar, 2020
Reviewers invited
Invitations sent on 26 Mar, 2020
Reviewer #1 agreed
On 26 Mar, 2020
Reviewer #2 agreed
On 26 Mar, 2020
Reviewer #3 agreed
On 26 Mar, 2020
Editor assigned
On 24 Mar, 2020
First submitted
On 23 Mar, 2020
Submission checks complete
On 23 Mar, 2020
Editor invited
On 23 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Neurology
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See the published version of this preprint at Military Medical Research.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Sheng Yi
Nantong University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Military Medical Research.
Version 2
Posted 20 May, 2020
No community comments so far
Editorial decision: Accept
On 04 Nov, 2020
Review #1 received
Received 29 May, 2020
Review #3 received
Received 19 May, 2020
Reviewer #2 agreed
On 18 May, 2020
Reviewer #3 agreed
On 18 May, 2020
Review #2 received
Received 18 May, 2020
Reviewer #1 agreed
On 16 May, 2020
Reviewers invited
Invitations sent on 16 May, 2020
Editor assigned
On 10 May, 2020
Submission checks complete
On 09 May, 2020
Editor invited
On 09 May, 2020
No comments provided
Editorial decision: Minor revision
On 24 Apr, 2020
Review #3 received
Received 22 Apr, 2020
Reviewer #4 agreed
On 17 Apr, 2020
Review #1 received
Received 27 Mar, 2020
Review #2 received
Received 27 Mar, 2020
Reviewers invited
Invitations sent on 26 Mar, 2020
Reviewer #1 agreed
On 26 Mar, 2020
Reviewer #2 agreed
On 26 Mar, 2020
Reviewer #3 agreed
On 26 Mar, 2020
Editor assigned
On 24 Mar, 2020
First submitted
On 23 Mar, 2020
Submission checks complete
On 23 Mar, 2020
Editor invited
On 23 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Neurology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Military Medical Research.
Background: Cytokines are essential cellular modulators of a variety of physiological and pathological activities, including peripheral nerve repair and regeneration. However, the molecular changes of these cellular mediators after peripheral nerve injury are not well clarified. The study is aimed to discover critical cytokines for the regenerative process of injured peripheral nerves.
Methods: The sequencing data of the injured nerve stumps and the dorsal root ganglia (DRGs) of Sprague-Dawley (SD) rats subjected to sciatic nerve (SN) crush injury were analyzed to determine expression patterns of genes coding for cytokines. PCR experiments were used to validate the accuracy of sequencing data.
Results: A total of 46, 52, and 54 upstream cytokines were differentially expressed in SNs at 1 day, 4 days, and 7 days after nerve injury. And a total of 25, 28, and 34 upstream cytokines were differentially expressed in DRGs at these time points. The expression patterns of some essential upstream cytokines were displayed in a heatmap and validated by PCR experiment. Bioinformatic analysis of these differentially expressed upstream cytokines after nerve injury demonstrated that inflammatory and immune responses were significantly involved.
Conclusions: In summary, these findings provided an overview of the dynamic changes of cytokines in SNs and DRGs at different time points after rat nerve crush injury, elucidated the biological processes of differentially expressed cytokines, especially the important roles in inflammatory and immune responses after peripheral nerve injury, and thus might contribute to identification of potential treatments for peripheral nerve repair and regeneration.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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