Characteristics of cytokines in the sciatic nerve stumps and DRGs after rat sciatic nerve crush injury
Background: Cytokines are essential cellular modulators of a variety of physiological and pathological activities, including peripheral nerve repair and regeneration. However, the molecular changes of these cellular mediators after peripheral nerve injury are not well clarified. The study is aimed to discover critical cytokines for the regenerative process of injured peripheral nerves.
Methods: The sequencing data of the injured nerve stumps and the dorsal root ganglia (DRGs) of Sprague-Dawley (SD) rats subjected to sciatic nerve (SN) crush injury were analyzed to determine expression patterns of genes coding for cytokines. PCR experiments were used to validate the accuracy of sequencing data.
Results: A total of 46, 52, and 54 upstream cytokines were differentially expressed in SNs at 1 day, 4 days, and 7 days after nerve injury. And a total of 25, 28, and 34 upstream cytokines were differentially expressed in DRGs at these time points. The expression patterns of some essential upstream cytokines were displayed in a heatmap and validated by PCR experiment. Bioinformatic analysis of these differentially expressed upstream cytokines after nerve injury demonstrated that inflammatory and immune responses were significantly involved.
Conclusions: In summary, these findings provided an overview of the dynamic changes of cytokines in SNs and DRGs at different time points after rat nerve crush injury, elucidated the biological processes of differentially expressed cytokines, especially the important roles in inflammatory and immune responses after peripheral nerve injury, and thus might contribute to identification of potential treatments for peripheral nerve repair and regeneration.
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Posted 20 May, 2020
On 23 Nov, 2020
On 04 Nov, 2020
Received 29 May, 2020
Received 19 May, 2020
On 18 May, 2020
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Received 18 May, 2020
On 16 May, 2020
Invitations sent on 16 May, 2020
On 10 May, 2020
On 09 May, 2020
On 09 May, 2020
On 24 Apr, 2020
Received 22 Apr, 2020
On 17 Apr, 2020
Received 27 Mar, 2020
Received 27 Mar, 2020
Invitations sent on 26 Mar, 2020
On 26 Mar, 2020
On 26 Mar, 2020
On 26 Mar, 2020
On 24 Mar, 2020
On 23 Mar, 2020
On 23 Mar, 2020
On 23 Mar, 2020
Characteristics of cytokines in the sciatic nerve stumps and DRGs after rat sciatic nerve crush injury
Posted 20 May, 2020
On 23 Nov, 2020
On 04 Nov, 2020
Received 29 May, 2020
Received 19 May, 2020
On 18 May, 2020
On 18 May, 2020
Received 18 May, 2020
On 16 May, 2020
Invitations sent on 16 May, 2020
On 10 May, 2020
On 09 May, 2020
On 09 May, 2020
On 24 Apr, 2020
Received 22 Apr, 2020
On 17 Apr, 2020
Received 27 Mar, 2020
Received 27 Mar, 2020
Invitations sent on 26 Mar, 2020
On 26 Mar, 2020
On 26 Mar, 2020
On 26 Mar, 2020
On 24 Mar, 2020
On 23 Mar, 2020
On 23 Mar, 2020
On 23 Mar, 2020
Background: Cytokines are essential cellular modulators of a variety of physiological and pathological activities, including peripheral nerve repair and regeneration. However, the molecular changes of these cellular mediators after peripheral nerve injury are not well clarified. The study is aimed to discover critical cytokines for the regenerative process of injured peripheral nerves.
Methods: The sequencing data of the injured nerve stumps and the dorsal root ganglia (DRGs) of Sprague-Dawley (SD) rats subjected to sciatic nerve (SN) crush injury were analyzed to determine expression patterns of genes coding for cytokines. PCR experiments were used to validate the accuracy of sequencing data.
Results: A total of 46, 52, and 54 upstream cytokines were differentially expressed in SNs at 1 day, 4 days, and 7 days after nerve injury. And a total of 25, 28, and 34 upstream cytokines were differentially expressed in DRGs at these time points. The expression patterns of some essential upstream cytokines were displayed in a heatmap and validated by PCR experiment. Bioinformatic analysis of these differentially expressed upstream cytokines after nerve injury demonstrated that inflammatory and immune responses were significantly involved.
Conclusions: In summary, these findings provided an overview of the dynamic changes of cytokines in SNs and DRGs at different time points after rat nerve crush injury, elucidated the biological processes of differentially expressed cytokines, especially the important roles in inflammatory and immune responses after peripheral nerve injury, and thus might contribute to identification of potential treatments for peripheral nerve repair and regeneration.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6