Basic patient and tumour characteristics are given in Table 1. In the examined samples, a total number of 2.589.019 microorganisms could be identified. In healthy kidney tissue, 528.795 microorganisms (mean 105.759, median 141.014, SD 80.455,217) and in malignant tissue 2.060.224 microorganisms (mean 412.045, median 179.774, SD 438.586,893) were isolated. Numbers for each sample are shown in Table 1. A plethora of microorganisms was found, with significant differences between benign and malignant renal tissue (p< 0.0001) (Fig. 1). We isolated 3 domains, 15 phyla, 16 classes, 19 orders, 27 families, 28 genera and 30 species of microorganisms. In the domain of the archeae we found two phyla, in the domain of the eukaryota 6 phyla, in the domain of the bacteria we found 7 phyla. The distribution of microorganisms shows differences in benign and malign tissue (Fig. 2).
The following microorganisms were found in healthy tissue only: Terrabacteria (phylum), Stenosarchaea (phylum), Microbacterium (genus), Pelomonas (genus), Staphylococcus (genus), Leuconostoc garlicum (species), Corynebacterium vitaeruminis (species), Anaerococcus nagyae (species), Ethanoligenens harbinense (species), Neisseria bacilliformis (species), Thermicanus aegyptius (species) and Leuconostoc mesenteroides (species).
Microorganisms that appeared in cancer tissue only were: Cyanophora paradoxa (species), Spirosoma navajo (species), Phaeocystis antarctica (species), Euglena mutabilis (species) and Mycoplasma vulturii (species).
Of the microorganisms found in both tissue types, the following were particularly frequent in cancer tissue (p< 0.005): Aeromonas salmonicida (species), Pseudoalteromonas haloplanktis (species), Parageobacillus toebii (species), Trachelomonas volvocinopsis (species), Mycoplasma mycoides (species) and Halomicrobium mukohataei (species).
The Bray Curtis dissimilarity showed a clear cluster of the microbiome of the benign tissue in the PCo diagram (Fig. 3).