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Research
Drp1 is widely, yet heterogeneously, distributed in the mouse central nervous system
Ya-Yun Wang
Fourth Military Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objectives: Drp1 is widely expressed in the mouse central nervous system and plays a role in inducing the mitochondrial fission process. Many diseases are associated with Drp1 and mitochondria. However, since the exact distribution of Drp1 has not been specifically observed, it is difficult to determine the impact of anti-Drp1 molecules on the human body. Clarifying the specific Drp1 distribution could be a good approach to targeted treatment or prognosis.
Methods: We visualized the distribution of Drp1 in different brain regions and explicated the relationship between Drp1 and mitochondria. GAD67-GFP knock-in mice were utilized to detect the expression patterns of Drp1 in GABAergic neurons. We also further analyzed Drp1 expression in human malignant glioma tissue.
Results : Drp1 was widely but heterogeneously distributed in the central nervous system. Further observation indicated that Drp1 was highly and heterogeneously expressed in inhibitory neurons. Under transmission electron microscopy, the distribution of Drp1 was higher in dendrites than other areas in neurons, and only a small amount of Drp1 was localized in mitochondria. In human malignant glioma, the fluorescence intensity of Drp1 increased from grade I-III, while grade IV showed a declining trend.
Conclusion: In this study, we observed a wide heterogeneous distribution of Drp1 in the central nervous system, which might be related to the occurrence and development of neurologic disease. We hope that the relationship between Drp1 and mitochondria may will to therapeutic guidance in the clinic.
Keywords
Mitochondria, Drp1, GABAergic neuron, glioma
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CURRENT STATUS: Under Review
Version 3
Posted 03 Jun, 2020
No community comments so far
Editor assigned
On 26 May, 2020
Submission checks complete
On 25 May, 2020
Editor invited
On 25 May, 2020
No comments provided
Review #2 received
Received 20 May, 2020
Editorial decision: Minor revision
On 20 May, 2020
Review #1 received
Received 19 May, 2020
Reviewers invited
Invitations sent on 14 May, 2020
Reviewer #1 agreed
On 14 May, 2020
Reviewer #2 agreed
On 14 May, 2020
Editor assigned
On 14 May, 2020
Submission checks complete
On 13 May, 2020
Editor invited
On 13 May, 2020
No comments provided
Editorial decision: Major revision
On 16 Apr, 2020
Review #2 received
Received 14 Apr, 2020
Review #1 received
Received 05 Apr, 2020
Reviewer #2 agreed
On 02 Apr, 2020
Reviewers invited
Invitations sent on 25 Mar, 2020
Reviewer #1 agreed
On 25 Mar, 2020
Editor assigned
On 23 Mar, 2020
First submitted
On 22 Mar, 2020
Submission checks complete
On 22 Mar, 2020
Editor invited
On 22 Mar, 2020
Metrics
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Subject Areas
Cellular & Molecular Neuroscience
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This preprint is under consideration at Molecular Brain. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Drp1 is widely, yet heterogeneously, distributed in the mouse central nervous system
Ya-Yun Wang
Fourth Military Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 3
Posted 03 Jun, 2020
No community comments so far
Editor assigned
On 26 May, 2020
Submission checks complete
On 25 May, 2020
Editor invited
On 25 May, 2020
No comments provided
Review #2 received
Received 20 May, 2020
Editorial decision: Minor revision
On 20 May, 2020
Review #1 received
Received 19 May, 2020
Reviewers invited
Invitations sent on 14 May, 2020
Reviewer #1 agreed
On 14 May, 2020
Reviewer #2 agreed
On 14 May, 2020
Editor assigned
On 14 May, 2020
Submission checks complete
On 13 May, 2020
Editor invited
On 13 May, 2020
No comments provided
Editorial decision: Major revision
On 16 Apr, 2020
Review #2 received
Received 14 Apr, 2020
Review #1 received
Received 05 Apr, 2020
Reviewer #2 agreed
On 02 Apr, 2020
Reviewers invited
Invitations sent on 25 Mar, 2020
Reviewer #1 agreed
On 25 Mar, 2020
Editor assigned
On 23 Mar, 2020
First submitted
On 22 Mar, 2020
Submission checks complete
On 22 Mar, 2020
Editor invited
On 22 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cellular & Molecular Neuroscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objectives: Drp1 is widely expressed in the mouse central nervous system and plays a role in inducing the mitochondrial fission process. Many diseases are associated with Drp1 and mitochondria. However, since the exact distribution of Drp1 has not been specifically observed, it is difficult to determine the impact of anti-Drp1 molecules on the human body. Clarifying the specific Drp1 distribution could be a good approach to targeted treatment or prognosis.
Methods: We visualized the distribution of Drp1 in different brain regions and explicated the relationship between Drp1 and mitochondria. GAD67-GFP knock-in mice were utilized to detect the expression patterns of Drp1 in GABAergic neurons. We also further analyzed Drp1 expression in human malignant glioma tissue.
Results : Drp1 was widely but heterogeneously distributed in the central nervous system. Further observation indicated that Drp1 was highly and heterogeneously expressed in inhibitory neurons. Under transmission electron microscopy, the distribution of Drp1 was higher in dendrites than other areas in neurons, and only a small amount of Drp1 was localized in mitochondria. In human malignant glioma, the fluorescence intensity of Drp1 increased from grade I-III, while grade IV showed a declining trend.
Conclusion: In this study, we observed a wide heterogeneous distribution of Drp1 in the central nervous system, which might be related to the occurrence and development of neurologic disease. We hope that the relationship between Drp1 and mitochondria may will to therapeutic guidance in the clinic.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10