Ethics approval
All experimental procedures were approved by the Ethics Commission on Animal Use (CEUA) at the Federal University of Rio Grande (FURG), protocol number 23116.007707/2021-00.
Consent to participate
Not applicable.
Human Ethics
Not applicable.
Consent for publication
All authors approved the final draft for publication.
Availability of supporting data
The data that support the findings of the present study are available on request from the corresponding author.
Competing interests
The authors declare no competing interests.
Funding
V.S.L. Nóbrega is a graduate student, and V.S. Pedrosa is post-doctoral at Aquaculture Program at FURG and are supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES Finance Code 001. L. A. Sampaio and L. A. Romano are researcher fellows by the Research Brazilian Agency CNPq.
Authors contributions
VSL Nóbrega, ANRombenso, LA Sampaio, RV Rodrigues - designed the experiment; VSL Nóbrega - performed the experiment; VF Pedrosa, LA Romano, VSL Nóbrega - performed the hematological and histochemistry analyzes; VSL Nóbrega and RV Rodrigues - performed the statistical analyses; VSL Nóbrega, VF Pedrosa, RV Rodrigues - wrote the main manuscript; All authors reviewed the manuscript.
Acknowledgements
The author would like to thanks all the staff from Laboratório de Piscicultura Estuarina e Marinha – FURG, Maricultura Costa Verde (especially Patrícia Merlin and Carlos Kazuo), and Flávia Calixto - veterinarian from Fundação Instituto de Pesca do Estado do Rio de Janeiro – FIPERJ for their assistance during the experimental procedures.
Authors information
Victor Santos Lira da Nóbrega1,2, Artur Nishioka Rombenso3, Virgínia Fonseca Pedrosa1,4, Luis Alberto Romano, Luís André Sampaio1,2, Ricardo Vieira Rodrigues1,2
1Programa de Pós-graduação em Aquicultura, Universidade Federal do Rio Grande – FURG, Rio Grande, Brazil
2Universidade Federal do Rio Grande – FURG, Instituto de Oceanografia, Laboratório de Piscicultura Estuarina e Marinha, Rio Grande, RS, 96201–900, Brazil
3CSIRO, Agriculture and Food, Aquaculture Program, Bribie Island Research Centre, Woorim, QLD 4507, Australia
4Universidade Federal do Rio Grande – FURG, Instituto de Oceanografia, Laboratório de Imunologia e Patologia de Organismos Aquáticos, Rio Grande, RS, 96201–900, Brazil