Sulfate-reducing bacteria (SRB) are easily inhibited by heavy metal ions and low pH when treating AMD, so iron-carbon microelectrolysis (IC-ME) was used to enhance SRB activity. The remediation AMD dynamic experiments were carried out by constructing seven groups of IC-ME bioreactors with different mass ratios (4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4), and the variation of microbial flora under IC-ME enhancement was explored by high-throughput sequencing. The mechanism of IC-ME enhanced SRB reactor to repair AMD was revealed by ion concentration change along the reactor, SEM, EDS, XRD, and other methods. The results indicate that SRB in the IC-ME bioreactor maintain a good biological activity when repairing AMD, and had a stable ability of sulfate removal. When the mass ratio of iron to carbon is 3:1, the removal rate of SO4 2-is 98.2%, and the removal rates of Cu2+ , Zn2+ , Mn2+ , and TFe were all above 99 %. The effluent was clear and the substrate utilization was less, which was better than that of other reactors. SO4 2-was reduced to S 2-by SRB and precipitated with metal ions; Cu2+ was mainly replaced by elemental copper deposited on the iron surface; Zn2+ was mainly removed in the form of sulfide and hydroxide precipitation; Mn2+ was mainly removed by manganese carbonate precipitation and iron ion coprecipitation; TFe was removed by oxide, hydroxide, and flocculation. Biocommunity analysis showed that SRB in the IC-ME bioreactor was not a single genus, and its relative abundance was better than control groups, with higher system stability. This study confirmed that the IC-ME enhanced SRB as a low-energy method for AMD will bring extensive application prospects.