GhCIPK6 Increases Salt Tolerance in Transgenic Upland Cotton by Involving in ROS Scavenging and MAPK Signaling Pathways
Background: Salt stress is one of the most damaging abiotic stresses in production of Upland cotton ( Gossypium hirsutum ). Upland cotton is defined as a medium salt-tolerant crop. Salinity hinders root development, shoots growth, and reduces the fiber quality.
Results: Our previous study verified a GhCIPK6 gene response to salt stress in G. hirsutum . The homologs of GhCIPK6 were analyzed in A 2 ( G. arboreum ), D 5 ( G . raimondii ), and AD 1 ( G . hirsutum ) genomes. GhCIPK6 localized to the vacuole and cell membrane. The GhCBL1-GhCIPK6 and GhCBL8-GhCIPK6 complexes localized to the nucleus and cytomembrane. Overexpression of GhCIPK6 enhanced expression levels of co-expressed genes induced by salt stress, which scavenged ROS and involved in MAPK signaling pathways verified by RNA-seq analysis. Water absorption capacity and cell membrane stability of seeds from GhCIPK6 overexpressed lines was higher than that of wild-type seeds during imbibed germination stage. The seed germination rates and seedling field emergence percentages of GhCIPK6 overexpressed lines were higher than that of control line under salt stress. Moreover, overexpressing of GhCIPK6 in cotton increased lint percentage, and fiber length uniformity under salt stress.
Conclusions: We verified the function of GhCIPK6 by transformation and RNA-seq analysis. GhCIPK6 overexpressed lines exhibited higher tolerance to abiotic stresses, which functioned by involving in ROS scavenging and MAPK pathways. Therefore, GhCIPK6 has the potential for cotton breeding to improve stress-tolerance.
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Posted 29 May, 2020
On 14 Sep, 2020
Received 22 Jun, 2020
On 22 Jun, 2020
Received 17 Jun, 2020
On 01 Jun, 2020
Invitations sent on 29 May, 2020
On 29 May, 2020
On 20 May, 2020
On 19 May, 2020
On 19 May, 2020
Received 24 Apr, 2020
On 24 Apr, 2020
Received 20 Apr, 2020
On 03 Apr, 2020
Invitations sent on 30 Mar, 2020
On 30 Mar, 2020
On 18 Mar, 2020
On 17 Mar, 2020
On 17 Mar, 2020
GhCIPK6 Increases Salt Tolerance in Transgenic Upland Cotton by Involving in ROS Scavenging and MAPK Signaling Pathways
Posted 29 May, 2020
On 14 Sep, 2020
Received 22 Jun, 2020
On 22 Jun, 2020
Received 17 Jun, 2020
On 01 Jun, 2020
Invitations sent on 29 May, 2020
On 29 May, 2020
On 20 May, 2020
On 19 May, 2020
On 19 May, 2020
Received 24 Apr, 2020
On 24 Apr, 2020
Received 20 Apr, 2020
On 03 Apr, 2020
Invitations sent on 30 Mar, 2020
On 30 Mar, 2020
On 18 Mar, 2020
On 17 Mar, 2020
On 17 Mar, 2020
Background: Salt stress is one of the most damaging abiotic stresses in production of Upland cotton ( Gossypium hirsutum ). Upland cotton is defined as a medium salt-tolerant crop. Salinity hinders root development, shoots growth, and reduces the fiber quality.
Results: Our previous study verified a GhCIPK6 gene response to salt stress in G. hirsutum . The homologs of GhCIPK6 were analyzed in A 2 ( G. arboreum ), D 5 ( G . raimondii ), and AD 1 ( G . hirsutum ) genomes. GhCIPK6 localized to the vacuole and cell membrane. The GhCBL1-GhCIPK6 and GhCBL8-GhCIPK6 complexes localized to the nucleus and cytomembrane. Overexpression of GhCIPK6 enhanced expression levels of co-expressed genes induced by salt stress, which scavenged ROS and involved in MAPK signaling pathways verified by RNA-seq analysis. Water absorption capacity and cell membrane stability of seeds from GhCIPK6 overexpressed lines was higher than that of wild-type seeds during imbibed germination stage. The seed germination rates and seedling field emergence percentages of GhCIPK6 overexpressed lines were higher than that of control line under salt stress. Moreover, overexpressing of GhCIPK6 in cotton increased lint percentage, and fiber length uniformity under salt stress.
Conclusions: We verified the function of GhCIPK6 by transformation and RNA-seq analysis. GhCIPK6 overexpressed lines exhibited higher tolerance to abiotic stresses, which functioned by involving in ROS scavenging and MAPK pathways. Therefore, GhCIPK6 has the potential for cotton breeding to improve stress-tolerance.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10