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GhCIPK6 Increases Salt Tolerance in Transgenic Upland Cotton by Involving in ROS Scavenging and MAPK Signaling Pathways
Jinping Hua
China Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4660-9244
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background: Salt stress is one of the most damaging abiotic stresses in production of Upland cotton ( Gossypium hirsutum ). Upland cotton is defined as a medium salt-tolerant crop. Salinity hinders root development, shoots growth, and reduces the fiber quality.
Results: Our previous study verified a GhCIPK6 gene response to salt stress in G. hirsutum . The homologs of GhCIPK6 were analyzed in A 2 ( G. arboreum ), D 5 ( G . raimondii ), and AD 1 ( G . hirsutum ) genomes. GhCIPK6 localized to the vacuole and cell membrane. The GhCBL1-GhCIPK6 and GhCBL8-GhCIPK6 complexes localized to the nucleus and cytomembrane. Overexpression of GhCIPK6 enhanced expression levels of co-expressed genes induced by salt stress, which scavenged ROS and involved in MAPK signaling pathways verified by RNA-seq analysis. Water absorption capacity and cell membrane stability of seeds from GhCIPK6 overexpressed lines was higher than that of wild-type seeds during imbibed germination stage. The seed germination rates and seedling field emergence percentages of GhCIPK6 overexpressed lines were higher than that of control line under salt stress. Moreover, overexpressing of GhCIPK6 in cotton increased lint percentage, and fiber length uniformity under salt stress.
Conclusions: We verified the function of GhCIPK6 by transformation and RNA-seq analysis. GhCIPK6 overexpressed lines exhibited higher tolerance to abiotic stresses, which functioned by involving in ROS scavenging and MAPK pathways. Therefore, GhCIPK6 has the potential for cotton breeding to improve stress-tolerance.
Keywords
CIPK, Salt stress, Co-expression, Upland cotton, Signaling pathway
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CURRENT STATUS: Published
Version 2
Posted 29 May, 2020
Published
On 14 Sep, 2020
No community comments so far
Review #2 received
Received 22 Jun, 2020
Editorial decision: Minor revision
On 22 Jun, 2020
Review #1 received
Received 17 Jun, 2020
Reviewer #2 agreed
On 01 Jun, 2020
Reviewers invited
Invitations sent on 29 May, 2020
Reviewer #1 agreed
On 29 May, 2020
Editor assigned
On 20 May, 2020
Submission checks complete
On 19 May, 2020
Editor invited
On 19 May, 2020
No comments provided
Review #2 received
Received 24 Apr, 2020
Editorial decision: Major revision
On 24 Apr, 2020
Review #1 received
Received 20 Apr, 2020
Reviewer #2 agreed
On 03 Apr, 2020
Reviewers invited
Invitations sent on 30 Mar, 2020
Reviewer #1 agreed
On 30 Mar, 2020
Editor assigned
On 18 Mar, 2020
Submission checks complete
On 17 Mar, 2020
Editor invited
On 17 Mar, 2020
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
GhCIPK6 Increases Salt Tolerance in Transgenic Upland Cotton by Involving in ROS Scavenging and MAPK Signaling Pathways
Jinping Hua
China Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4660-9244
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 29 May, 2020
Published
On 14 Sep, 2020
No community comments so far
Review #2 received
Received 22 Jun, 2020
Editorial decision: Minor revision
On 22 Jun, 2020
Review #1 received
Received 17 Jun, 2020
Reviewer #2 agreed
On 01 Jun, 2020
Reviewers invited
Invitations sent on 29 May, 2020
Reviewer #1 agreed
On 29 May, 2020
Editor assigned
On 20 May, 2020
Submission checks complete
On 19 May, 2020
Editor invited
On 19 May, 2020
No comments provided
Review #2 received
Received 24 Apr, 2020
Editorial decision: Major revision
On 24 Apr, 2020
Review #1 received
Received 20 Apr, 2020
Reviewer #2 agreed
On 03 Apr, 2020
Reviewers invited
Invitations sent on 30 Mar, 2020
Reviewer #1 agreed
On 30 Mar, 2020
Editor assigned
On 18 Mar, 2020
Submission checks complete
On 17 Mar, 2020
Editor invited
On 17 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background: Salt stress is one of the most damaging abiotic stresses in production of Upland cotton ( Gossypium hirsutum ). Upland cotton is defined as a medium salt-tolerant crop. Salinity hinders root development, shoots growth, and reduces the fiber quality.
Results: Our previous study verified a GhCIPK6 gene response to salt stress in G. hirsutum . The homologs of GhCIPK6 were analyzed in A 2 ( G. arboreum ), D 5 ( G . raimondii ), and AD 1 ( G . hirsutum ) genomes. GhCIPK6 localized to the vacuole and cell membrane. The GhCBL1-GhCIPK6 and GhCBL8-GhCIPK6 complexes localized to the nucleus and cytomembrane. Overexpression of GhCIPK6 enhanced expression levels of co-expressed genes induced by salt stress, which scavenged ROS and involved in MAPK signaling pathways verified by RNA-seq analysis. Water absorption capacity and cell membrane stability of seeds from GhCIPK6 overexpressed lines was higher than that of wild-type seeds during imbibed germination stage. The seed germination rates and seedling field emergence percentages of GhCIPK6 overexpressed lines were higher than that of control line under salt stress. Moreover, overexpressing of GhCIPK6 in cotton increased lint percentage, and fiber length uniformity under salt stress.
Conclusions: We verified the function of GhCIPK6 by transformation and RNA-seq analysis. GhCIPK6 overexpressed lines exhibited higher tolerance to abiotic stresses, which functioned by involving in ROS scavenging and MAPK pathways. Therefore, GhCIPK6 has the potential for cotton breeding to improve stress-tolerance.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10