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Research article
High genetic diversity and strong genetic structure of Strongyllodes variegatus populations in oilseed rape production areas of China
Shu-min Hou
Anhui Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3402-4943
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Strongyllodes variegatus (Fairmaire) is a major insect pest of oilseed rape in China. Despite its economic importance, the contribution of its population genetics in the development of any suitable protection control strategy for the management of oilseed rape crops is poorly studied. It is a much urgent need to prevent its spread to the rest of the world.
Results: Using the sequences of mitochondrial DNA cytochrome c oxidase subunit I (COI) and cytochrome b (Cytb) as genetic markers, we analyzed the population genetic diversity and structure of 437 individuals collected from 15 S. variegates populations located in different oilseed rape production areas in China. In addition, we estimated the demographic history using neutrality test and mismatch distribution analysis. The high level of genetic diversity was detected among the COI and Cytb sequences of S. variegates. The population structure analyses strongly suggested three distinct genetic and geographical regions in China with limited gene flow. The Mantel test showed that the genetic distance was greatly influenced by the geographical distance. The demographic analyses showed that S. variegates had experienced population fluctuation during the Pleistocene Epoch, which was likely to be related to the climatic changes.
Conclusion: Overall, these results demonstrate that the strong genetic structure of S. variegates populations in China, which is attributed by the isolation through the geographical distance among populations, their weak flight capacity and subsequent adaptation to the regional ecological conditions.
Keywords
Gene flow, Genetic differentiation, Haplotype, Oilseed rape, Population genetic pattern, Strongyllodes variegates
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Received 27 Aug, 2020
Reviewer #2 agreed
On 11 Aug, 2020
Review #2 received
Received 11 Aug, 2020
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On 07 Aug, 2020
Reviewers invited
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On 21 Jul, 2020
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Editor invited
On 20 Jul, 2020
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Editorial decision: Major revision
On 28 May, 2020
Review #2 received
Received 14 May, 2020
Review #1 received
Received 02 May, 2020
Reviewer #2 agreed
On 24 Apr, 2020
Reviewers invited
Invitations sent on 22 Apr, 2020
Reviewer #1 agreed
On 22 Apr, 2020
Editor assigned
On 30 Mar, 2020
Submission checks complete
On 29 Mar, 2020
Editor invited
On 29 Mar, 2020
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On 25 Mar, 2020
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Evolutionary Biology
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This preprint is under consideration at BMC Evolutionary Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
High genetic diversity and strong genetic structure of Strongyllodes variegatus populations in oilseed rape production areas of China
Shu-min Hou
Anhui Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3402-4943
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 3
Posted 24 Sep, 2020
No community comments so far
Editor assigned
On 24 Sep, 2020
Submission checks complete
On 23 Sep, 2020
Editor invited
On 23 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 01 Sep, 2020
Review #1 received
Received 27 Aug, 2020
Reviewer #2 agreed
On 11 Aug, 2020
Review #2 received
Received 11 Aug, 2020
Reviewer #1 agreed
On 07 Aug, 2020
Reviewers invited
Invitations sent on 06 Aug, 2020
Editor assigned
On 21 Jul, 2020
Submission checks complete
On 20 Jul, 2020
Editor invited
On 20 Jul, 2020
No comments provided
Editorial decision: Major revision
On 28 May, 2020
Review #2 received
Received 14 May, 2020
Review #1 received
Received 02 May, 2020
Reviewer #2 agreed
On 24 Apr, 2020
Reviewers invited
Invitations sent on 22 Apr, 2020
Reviewer #1 agreed
On 22 Apr, 2020
Editor assigned
On 30 Mar, 2020
Submission checks complete
On 29 Mar, 2020
Editor invited
On 29 Mar, 2020
First submitted
On 25 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Evolutionary Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Strongyllodes variegatus (Fairmaire) is a major insect pest of oilseed rape in China. Despite its economic importance, the contribution of its population genetics in the development of any suitable protection control strategy for the management of oilseed rape crops is poorly studied. It is a much urgent need to prevent its spread to the rest of the world.
Results: Using the sequences of mitochondrial DNA cytochrome c oxidase subunit I (COI) and cytochrome b (Cytb) as genetic markers, we analyzed the population genetic diversity and structure of 437 individuals collected from 15 S. variegates populations located in different oilseed rape production areas in China. In addition, we estimated the demographic history using neutrality test and mismatch distribution analysis. The high level of genetic diversity was detected among the COI and Cytb sequences of S. variegates. The population structure analyses strongly suggested three distinct genetic and geographical regions in China with limited gene flow. The Mantel test showed that the genetic distance was greatly influenced by the geographical distance. The demographic analyses showed that S. variegates had experienced population fluctuation during the Pleistocene Epoch, which was likely to be related to the climatic changes.
Conclusion: Overall, these results demonstrate that the strong genetic structure of S. variegates populations in China, which is attributed by the isolation through the geographical distance among populations, their weak flight capacity and subsequent adaptation to the regional ecological conditions.
Figure 1
Figure 2
Figure 3
Figure 4