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Exercise promotes satellite cell contribution to myofibers in a load-dependent manner
Gommaar D'Hulst
Eidgenossische Technische Hochschule Zurich
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5737-8627
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View the published version at Skeletal Muscle.
Background Satellite cells (SCs) are required for muscle repair following injury and are involved in muscle remodeling upon muscular contractions. Exercise stimulates SC accumulation and myonuclear accretion. To what extent exercise training at different mechanical loads drive SC contribution to myonuclei however is unknown.
Results By performing SC fate tracing experiments, we show that 8-weeks of voluntary wheel running increased SC contribution to myofibers in mouse plantar flexor muscles in a load-dependent but fiber type-independent manner. Increased SC fusion however was not exclusively linked to muscle hypertrophy as wheel running without external load substantially increased SC fusion in the absence of fiber hypertrophy. Due to nuclear propagation, nuclear fluorescent fate tracing mouse models were inadequate to quantify SC contribution to myonuclei. Ultimately, by performing fate tracing at the DNA level, we show that SC contribution mirrors myonuclear accretion during exercise.
Conclusions Collectively, mechanical load during exercise independently promotes SC contribution to existing myofibers. Also, due to propagation of nuclear fluorescent reporter proteins, our data warrant caution for the use of exisiting reporter mouse models for the quantitative evaluation of satellite cell contribution to myonuclei.
Keywords
Satellite cell, Fusion, Exercise, Load, Running, Fate Tracing, Myonuclei
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CURRENT STATUS: Published
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Posted 15 Jun, 2020
Published
On 09 Jul, 2020
No community comments so far
Editorial decision: Accept
On 15 Jun, 2020
Editor assigned
On 11 Jun, 2020
Submission checks complete
On 10 Jun, 2020
Editor invited
On 10 Jun, 2020
No comments provided
Review #2 received
Received 10 Jun, 2020
Editorial decision: Provisional Accept
On 10 Jun, 2020
Reviewer #2 agreed
On 02 Jun, 2020
Reviewers invited
Invitations sent on 01 Jun, 2020
Reviewer #1 agreed
On 01 Jun, 2020
Review #1 received
Received 01 Jun, 2020
Editor assigned
On 25 May, 2020
Submission checks complete
On 24 May, 2020
Editor invited
On 24 May, 2020
No comments provided
Editorial decision: Major revision
On 25 Apr, 2020
Review #2 received
Received 14 Apr, 2020
Reviewer #3 agreed
On 04 Apr, 2020
Review #1 received
Received 03 Apr, 2020
Reviewer #2 agreed
On 01 Apr, 2020
Reviewers invited
Invitations sent on 01 Apr, 2020
Reviewer #1 agreed
On 01 Apr, 2020
Editor assigned
On 30 Mar, 2020
Submission checks complete
On 29 Mar, 2020
Editor invited
On 29 Mar, 2020
First submitted
On 27 Mar, 2020
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Subject Areas
Sports Medicine and Kinesiology
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This preprint is under consideration at Skeletal Muscle. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Exercise promotes satellite cell contribution to myofibers in a load-dependent manner
Gommaar D'Hulst
Eidgenossische Technische Hochschule Zurich
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5737-8627
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 15 Jun, 2020
Published
On 09 Jul, 2020
No community comments so far
Editorial decision: Accept
On 15 Jun, 2020
Editor assigned
On 11 Jun, 2020
Submission checks complete
On 10 Jun, 2020
Editor invited
On 10 Jun, 2020
No comments provided
Review #2 received
Received 10 Jun, 2020
Editorial decision: Provisional Accept
On 10 Jun, 2020
Reviewer #2 agreed
On 02 Jun, 2020
Reviewers invited
Invitations sent on 01 Jun, 2020
Reviewer #1 agreed
On 01 Jun, 2020
Review #1 received
Received 01 Jun, 2020
Editor assigned
On 25 May, 2020
Submission checks complete
On 24 May, 2020
Editor invited
On 24 May, 2020
No comments provided
Editorial decision: Major revision
On 25 Apr, 2020
Review #2 received
Received 14 Apr, 2020
Reviewer #3 agreed
On 04 Apr, 2020
Review #1 received
Received 03 Apr, 2020
Reviewer #2 agreed
On 01 Apr, 2020
Reviewers invited
Invitations sent on 01 Apr, 2020
Reviewer #1 agreed
On 01 Apr, 2020
Editor assigned
On 30 Mar, 2020
Submission checks complete
On 29 Mar, 2020
Editor invited
On 29 Mar, 2020
First submitted
On 27 Mar, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Sports Medicine and Kinesiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Skeletal Muscle.
Background Satellite cells (SCs) are required for muscle repair following injury and are involved in muscle remodeling upon muscular contractions. Exercise stimulates SC accumulation and myonuclear accretion. To what extent exercise training at different mechanical loads drive SC contribution to myonuclei however is unknown.
Results By performing SC fate tracing experiments, we show that 8-weeks of voluntary wheel running increased SC contribution to myofibers in mouse plantar flexor muscles in a load-dependent but fiber type-independent manner. Increased SC fusion however was not exclusively linked to muscle hypertrophy as wheel running without external load substantially increased SC fusion in the absence of fiber hypertrophy. Due to nuclear propagation, nuclear fluorescent fate tracing mouse models were inadequate to quantify SC contribution to myonuclei. Ultimately, by performing fate tracing at the DNA level, we show that SC contribution mirrors myonuclear accretion during exercise.
Conclusions Collectively, mechanical load during exercise independently promotes SC contribution to existing myofibers. Also, due to propagation of nuclear fluorescent reporter proteins, our data warrant caution for the use of exisiting reporter mouse models for the quantitative evaluation of satellite cell contribution to myonuclei.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5