Although blood transfusion is one of the known therapeutic interventions that cuts across a number of clinical disciplines. It is necessary to test all intending blood donors for HIV infection before donation. The aim of this study was to determine the prevalence of HIV among blood donors at PIMS Blood Bank, Islamabad,Pakistan. A retrospective study was conducted in PIMS Blood Bank through the year 2022. Sera from blood donors were tested for the detection of Anti HIV by using Abott ELISA. Data were abstracted from records and analyzed using Microsoft Excel sheet. From the total of 18500 screened blood samples collected, the prevalence of HIV in blood donors in the blood bank was 0.001% in the 2022 from January to July. The age groups 16-23 and 33-41 were the highest prevalence and the age group 45-54 was the lowest prevalence of HIV infection. The prevalence of HIV among male was higher than in female donors . The trend of HIV infection was decreasing for both male and female blood donors.The prevalence of HIV infections among blood donors is still high in this study setting, and needs constant monitoring to evaluate prevention and control strategies to reduce the burden of transfusion-transmissible HIV infections.