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Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
Jamie Jennifer Kopper
Iowa State University College of Veterinary Medicine
Corresponding Author
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Objective: Vancomycin resistant enterococci (VRE) are of significant public health concern. The identification of VRE in livestock and food has increased. The objective of this study was to determine if the transferrable vancomycin resistance genes vanA and vanB were present in probiotics marketed for use in animals that claimed to contain Enterococcus spp.
Results: Of the 40 products selected, Enterococcus spp. DNA was successfully extracted from 36 products. Of these 36 products with enterococcal DNA, 2 (6%) had a PCR product consistent with vanA which was confirmed by sequencing. None of the products appeared to contain vanB.
Keywords
Antimicrobial resistance, enterococcus, vancomycin, probiotic
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CURRENT STATUS: Under Review
Version 2
Posted 02 Jun, 2020
No community comments so far
Editor assigned
On 19 May, 2020
Submission checks complete
On 18 May, 2020
Editor invited
On 18 May, 2020
No comments provided
Editorial decision: Major revision
On 20 Apr, 2020
Review #1 received
Received 15 Apr, 2020
Reviewer #1 agreed
On 11 Apr, 2020
Editor assigned
On 10 Apr, 2020
Reviewers invited
Invitations sent on 10 Apr, 2020
Submission checks complete
On 09 Apr, 2020
Editor invited
On 09 Apr, 2020
First submitted
On 01 Apr, 2020
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This preprint is under consideration at BMC Research Notes. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Evaluation of commercial veterinary probiotics containing enterococci for transferrable vancomycin resistance genes
Jamie Jennifer Kopper
Iowa State University College of Veterinary Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 02 Jun, 2020
No community comments so far
Editor assigned
On 19 May, 2020
Submission checks complete
On 18 May, 2020
Editor invited
On 18 May, 2020
No comments provided
Editorial decision: Major revision
On 20 Apr, 2020
Review #1 received
Received 15 Apr, 2020
Reviewer #1 agreed
On 11 Apr, 2020
Editor assigned
On 10 Apr, 2020
Reviewers invited
Invitations sent on 10 Apr, 2020
Submission checks complete
On 09 Apr, 2020
Editor invited
On 09 Apr, 2020
First submitted
On 01 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective: Vancomycin resistant enterococci (VRE) are of significant public health concern. The identification of VRE in livestock and food has increased. The objective of this study was to determine if the transferrable vancomycin resistance genes vanA and vanB were present in probiotics marketed for use in animals that claimed to contain Enterococcus spp.
Results: Of the 40 products selected, Enterococcus spp. DNA was successfully extracted from 36 products. Of these 36 products with enterococcal DNA, 2 (6%) had a PCR product consistent with vanA which was confirmed by sequencing. None of the products appeared to contain vanB.